LS174T

87060401, human colon, Epithelial

• Synonim(y): LS-174-T Cells, LS-174T Cells, LS174 Cells
• Kod UNSPSC: 41106514
• pochodzenie biologiczne: human colon
• tryb wzrostu: Adherent
• kariotyp: Modal no. 45 (2n
• morfologia: Epithelial
• produkty: Not specified
• receptory: Not specified
• powiązane choroby: cancer

Właściwości

• Nazwa produktu: LS174T, 87060401
• pochodzenie biologiczne: human colon
• tryb wzrostu: Adherent
• kariotyp: Modal no. 45 (2n
• morfologia: Epithelial
• produkty: Not specified
• receptory: Not specified
• metody: cell culture | mammalian: suitable
• powiązane choroby: cancer
• Warunki transportu: dry ice
• temp. przechowywania: −196°C

Opis

Pochodzenie linii komórkowej

Human Caucasian colon adenocarcinoma

Opis linii komórkowej

A trypsinised variant of LS180 (Sigma Catalogue number. 87021202), both lines being derived from a 58 year old female with Dukes type B adenocarcinoma of the colon. The cells have microvilli and intracytoplasmic vacuoles. The cells are tumorigenic in nude mice.

Zastosowanie

LS174T is part of the three-dimensional (3D) heterotypic spheroid model developed to assess cancer immunotherapy agents in vitro. It has been used to study the interaction of enterohaemorrhagic Escherichia coli (EHEC) with the intestinal mucus layer produced by LS174T cells.

pożywka hodowlana

EMEM (EBSS) + 2mM Glutamine + 1% Non Essential Amino Acids (NEAA) + 10% Foetal Bovine Serum (FBS).

Rutyna subkultury

Split sub-confluent cultures (70-80%) 1:2 to 1:5 i.e. seeding at 2-5x10,000 cells/cm² using 0.25% trypsin or trypsin/EDTA; 5% CO₂; 37°C May take up to 7 days for cells to attach. Cells grow in islands and tend to pile on top of each other. Some cells ma

Inne uwagi

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