54SCC3

8062561, Mouse x Rat Hybridoma nerve, oligopolar

• Kod UNSPSC: 41106514

Właściwości

• product name: 54SCC3, 08062561
• pochodzenie biologiczne: Mouse x Rat Hybridoma nerve
• tryb wzrostu: Adherent
• kariotyp: Modal No. varied with passage see reference
• morfologia: oligopolar
• produkty: Not specified
• receptory: Not specified
• metody: cell culture | mammalian: suitable
• powiązane choroby: cancer
• Warunki transportu: dry ice
• temp. przechowywania: −196°C

Opis

Pochodzenie linii komórkowej

Rat glioma x mouse L cell fibroblast hybrid

Opis linii komórkowej

Hybrid generated by fusion of bromodeoxyuridine-resistant mutant of rat glioma cell line C6 (C6-BU-1, Sigma Catalogue number 08062563) and the 6-thioguanine-resistant mouse L cell fibroblast line A9 (Sigma Catalogue number 08062526) using inactivated Sendai virus. Cells formed from such a fusion are multipolar and extend small processes.

pożywka hodowlana

DMEM + 2 mM glutamine + 10% Foetal Bovine Serum (FBS) + HAT (0.1 mM hypoxanthine, 10 μM aminopterin and 16 μM thymidine) Cells can be cultured without HAT provided they were cultured in between in HT medium for 3-4 days

Rutyna subkultury

Split sub-confluent cultures (70-80%) 1:3 to 1:6 i.e. seeding at 2-4x10,000 cells/cm² using 0.05% trypsin/EDTA; 5% CO₂; 37°C.

Inne uwagi

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