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C6079
Collagenase + protease inhibitor
2-5 FALGPA units/mg solid, ≥800 CDU/mg solid
Synonim(y):
Preparat kolagenazy, Roztwór inhibitora proteazy
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About This Item
Polecane produkty
![N-[3-(2-Furyl)acryloyl]-Leu-Gly-Pro-Ala](/deepweb/assets/sigmaaldrich/product/structures/805/876/96b5fb57-71c8-4c6b-b5d2-fafe7374cd85/640/96b5fb57-71c8-4c6b-b5d2-fafe7374cd85.png)
pochodzenie biologiczne
bacterial (Clostridium histolyticum)
Poziom jakości
Formularz
solid
aktywność właściwa
≥800 CDU/mg solid
2-5 FALGPA units/mg solid
masa cząsteczkowa
68-130 kDa
Warunki transportu
dry ice
temp. przechowywania
−20°C
informacje o genach
rat ... LOC296948(296948)
Zastosowanie
Collagenase with protease inhibitor has been used in a study to assess the association of immune system gene polymorphisms with quantitative features. It has also been used in a study to investigate the quantitative structure-activity relationship study on Clostridium histolyticum collagenase inhibitors. The enzyme from sigma has been used in the isolation of porcine pancreatic islets. It has also been used in the isolation of pancreatic-infiltrating lymphocytes from mice.
Działania biochem./fizjol.
Effective release of cells from tissue requires the action of both collagenase enzymes and the neutral protease. Collagenase is activated by four grams of calcium (Ca2+) per mole of the enzyme. The culture filtrate is thought to contain at least 7 different proteases ranging in molecular weight from 68-130 kDa. The pH optimum is 6.3-8.8. The enzyme is typically used to digest the connective components in tissue samples to liberate individual cells. Collagenase treatment can cause some cells to die. Typically, concentrations varying from 0.1 to 5 mg/mL are used for digestion. The duration of reaction can vary from 15 minutes to several hours for satisfactory cell dissociation without causing too much cell death. Zn2+ is required for activity. This product is used if the collagenase does not require a significant protease activity.
Inne uwagi
Selected lots of collagenase Type XI blended with protease inhibitor to limit the tryptic enzyme activities in pancreatic tissue. The formulation was developed through collaboration with several outside laboratories.
Jakość
Also contains clostripain and non-specific neutral protease activities.
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Inhibitor
Numer produktu
Opis
Cennik
Hasło ostrzegawcze
Danger
Zwroty wskazujące rodzaj zagrożenia
Zwroty wskazujące środki ostrożności
Klasyfikacja zagrożeń
Acute Tox. 4 Dermal - Acute Tox. 4 Inhalation - Acute Tox. 4 Oral - Resp. Sens. 1 - Skin Sens. 1
Kod klasy składowania
11 - Combustible Solids
Klasa zagrożenia wodnego (WGK)
WGK 3
Temperatura zapłonu (°F)
Not applicable
Temperatura zapłonu (°C)
Not applicable
Środki ochrony indywidualnej
dust mask type N95 (US), Eyeshields, Faceshields, Gloves
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Beverly Duncan et al.
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S P Gupta et al.
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Goncharova, I., et al.
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C J Swanson et al.
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Protokoły
This procedure may be used for Collagenase products.
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