C5233

Carboxypeptidase B from human pancreas

50-55 units/mg protein carboxypeptidase B

• Synonim(y): Peptidyl-L-Lysine[L-arginine] hydrolase
• Numer CAS: 9025-24-5
• Numer EC enzymu: 3.4.17.2 (BRENDA, IUBMB)
• Numer MDL: MFCD00081476
• Kod UNSPSC: 12352204
• Identyfikator substancji w PubChem: 24892769
• NACRES: NA.54

Właściwości

• pochodzenie biologiczne: human pancreas
• Poziom jakości: 200
• Postać: solution
• aktywność właściwa: 50-55 units/mg protein carboxypeptidase B
• zanieczyszczenia: ≤0.2% chymotrypsin; ≤0.2% trypsin; ≤1 unit/mg protein carboxypeptidase A
• numer dostępu UniProt: P15086
• Warunki transportu: dry ice
• temp. przechowywania: −20°C
• InChI: 1S/C31H38N4O7S/c1-20(32-26(36)15-16-27(37)38)28(39)33-21(2)30(41)35-17-9-14-25(35)29(40)34-24(18-22-10-5-3-6-11-22)31(42)43-19-23-12-7-4-8-13-23/h3-8,10-13,20-21,24-25H,9,14-19H2,1-2H3,(H,32,36)(H,33,39)(H,34,40)(H,37,38)
• Klucz InChI: TWURVFFNODFJBJ-UHFFFAOYSA-N
• informacje o genach: human ... CPB1(1360)

Opis

Opis ogólny

Carboxypeptidase B is mapped to human chromosome 3q24. Carboxypeptidase B belongs to A/B subfamily of carboxypeptidases.

Zastosowanie

Carboxypeptidase B from Sigma has been used as a reference for assaying carboxypeptidase activity in lysed pituitary granules derived from the anterior and intermediate lobes of rat. The enzyme has also been used to digest plasma samples by removing C-terminal basic amino acids, to get a distinct band for each allotype during C4 electrophoresis.

Działania biochem./fizjol.

Carboxypeptidase B (or peptidyl-L-lysine (-L-arginine) hydrolase) catalyzes the hydrolysis of the basic amino acids, lysine, arginine, and ornithine from the C-terminal position of polypeptides. It has been shown to be a single polypeptide of 34,000 Da. Trypsin is capable of converting native enzyme to the active enzyme, carboxypeptidase B II in vitro. The optimum pH is found to be 9.0. The enzyme may be used for sequence analysis by successive cleavage of C-terminal basic amino acids. It can also be used as a serum marker for the diagnosis of acute pancreatitis.

Mutations in the carboxypeptidase B (CPB1) gene is implicated with increased susceptibility to pancreatic cancer development and progression. Elevated levels of CPB1 is associated with low grade breast tumors and lymph node positive grade 1 tumors.

Definicja jednostki

One unit will hydrolyze 1 μmole of hippuryl-L-arginine per minute at pH 7.7 at 25 °C

Postać fizyczna

Solution in 0.05 M NaOAc pH 5.0 + 1.0 M NaCl + 0.01% NaN₃

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Informacja o środkach bezpieczeństwa

• Kod klasy składowania: 12 - Non Combustible Liquids
• Klasa zagrożenia wodnego (WGK): WGK 1
• Temperatura zapłonu (°F): Not applicable
• Temperatura zapłonu (°C): Not applicable