C4585

Anti-β-Tubulin−Cy3 antibody, Mouse monoclonal

clone TUB 2.1, purified from hybridoma cell culture

• Synonim(y): Monoclonal Anti-β-Tubulin
• Kod UNSPSC: 12352203
• NACRES: NA.41

Właściwości

• pochodzenie biologiczne: mouse
• Poziom jakości: 200
• białko sprzężone: CY3 conjugate
• forma przeciwciała: purified from hybridoma cell culture
• rodzaj przeciwciała: primary antibodies
• klon: TUB 2.1, monoclonal
• Formularz: buffered aqueous solution
• masa cząsteczkowa: antigen 55 kDa
• reaktywność gatunkowa: human, rat, frog, moth, mouse, plant, rabbit, chicken, bovine, wheat, sea urchin, hamster
• warunki przechowywania: protect from light
• metody: direct immunofluorescence: 1:100 using cultured chicken fibroblasts or BHK cells
• izotyp: IgG1
• Warunki transportu: wet ice
• temp. przechowywania: 2-8°C
• docelowa modyfikacja potranslacyjna: unmodified
• informacje o genach: human ... TUBB(203068) , TUBB1(81027) , TUBB1(81027) , TUBB1(81027) , TUBB1(81027) , TUBB2A(7280) , TUBB2A(7280) , TUBB2A(7280) , TUBB2A(7280) , TUBB2C(10383) , TUBB2C(10383) , TUBB2C(10383) , TUBB2C(10383) ; mouse ... Tubb1(104068) , Tubb1(104068) , Tubb1(104068) , Tubb1(104068) , Tubb2a(22151) , Tubb2a(22151) , Tubb2a(22151) , Tubb2a(22151) , Tubb2c(227613) , Tubb2c(227613) , Tubb2c(227613) , Tubb2c(227613) , Tubb3(22152) ; rat ... Tubb2(29212) , Tubb2(29212) , Tubb2(29212) , Tubb2(29212) , Tubb2c(296554) , Tubb2c(296554) , Tubb2c(296554) , Tubb2c(296554)

Opis

Opis ogólny

Monoclonal Anti-b-Tubulin (mouse IgG1 isotype) is derived from the TUB2.1 hybridoma produced by the fusion of mouse myeloma cells and splenocytes from mouse immunized with purified rat brain tubulin. Tubulin is the major building block of microtubules. This intracellular cylindrical filamentous structure is present in almost all eukaryotic cells. Tubulin is a heterodimer, which consists of α-tubulin and β-tubulin; both subunits have a molecular weight of 55 kDa and share considerable homology. β-tubulin structure is characterized with the core of two β-sheets enclosed by α-helices.

Specyficzność

The antibody recognizes all five isoforms of β-tubulin (β1-β5). It reacts with the β-Lc and β-Sc fragments in the carboxy-terminal part of β−tubulin in immunoblotting. The antibody may be used to localize β−tubulin in cultured cells or tissue sections.

Immunogen

tubulin from rat brain.

Zastosowanie

Monoclonal Anti-β-Tubulin-Cy3 antibody produced in mouse has been used in:

• immunohistochemical assays
• immunofluorescence
• western blotting
• immunocytochemistry
• immunoblotting
• immunostaining

Działania biochem./fizjol.

Microtubules function as structural and mobile elements in mitosis, intracellular transport, flagellar movement and in the cytoskeleton. Mutation in the β-tubulin gene leads to various neuronal migration disorders such as lissencephaly, pachygyria and polymicrogyria malformations.

Postać fizyczna

Solution in 0.01 M phosphate buffered saline, pH 7.4, containing 1% bovine serum albumin and 15 mM sodium azide.

Informacje prawne

Cy is distributed under license from Amersham Biosciences Limited.

Oświadczenie o zrzeczeniu się odpowiedzialności

Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.

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Informacja o środkach bezpieczeństwa

• Kod klasy składowania: 10 - Combustible liquids
• Klasa zagrożenia wodnego (WGK): nwg
• Temperatura zapłonu (°F): Not applicable
• Temperatura zapłonu (°C): Not applicable
• Środki ochrony indywidualnej: Eyeshields, Gloves, multi-purpose combination respirator cartridge (US)