C3483
Cytochrome c from human heart
≥95% (SDS-PAGE), lyophilized powder
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About This Item
Polecane produkty
pochodzenie biologiczne
human heart
Poziom jakości
Próba
≥95% (SDS-PAGE)
Formularz
lyophilized powder
masa cząsteczkowa
11 kDa by SDS-PAGE
metody
cell culture | mammalian: suitable
mass spectrometry (MS): suitable
western blot (chemiluminescent): suitable
numer dostępu UniProt
temp. przechowywania
−20°C
informacje o genach
human ... CYCS(54205) , CYCSL1(157317)
Opis ogólny
Cytochrome c (CYCS) is mapped to human chromosome 7p15.3. CYCS is a heme protein containing three long α-helices. It undergoes domain swapping at the C-terminus and exists in dimeric, trimeric, and tetrameric forms. Insertion mutations in CYCS is implicated in acute myeloid leukemia. Mutations in CYCS modulates the release of proplatelets and decreases platelet production leading to thrombocytopenia 4.
Zastosowanie
Cytochrome c from human heart has been used for the generation of standard curves in the electrochemiluminescence assay for measurement of cytochrome C levels in plasma samples and in electrospray ionization mass spectrum (ESI-MS) analysis.
Cytochrome c has been identified as an important mediator in apoptotic pathways. The release of mitochondrial cytochrome c into the cytoplasm stimulates apoptosis and is commonly used as an indicator of the apoptotic process in the cell. Investigation on the effect of Paris Saponin I (PS I) on human gastric carcinoma cell growth (SGC7901 cells) have shown an elevated level cytoplasmic cytochrome c. Results are an inhibition of proliferation in SGC7901 cells by inducing mitochondria-dependent apoptosis through cytochrome c.
Działania biochem./fizjol.
Cytochrome c is a small heme protein that is a mobile electron carrier in the mitochondrial electron transport chain. It is also an initiator of apoptosis.
The ready fluctuation of cytochrome c within the cell between ferrous and ferric states, makes it an efficient biological electron-transporter. It plays a vital role in cellular oxidations in both plants and animals. Generally regarded as a universal catalyst of respiration, it forms the essential electron-bridge between the respirable substrates and oxygen.
The ready fluctuation of cytochrome c within the cell between ferrous and ferric states, makes it an efficient biological electron-transporter. It plays a vital role in cellular oxidations in both plants and animals. Generally regarded as a universal catalyst of respiration, it forms the essential electron-bridge between the respirable substrates and oxygen.
In addition to its function in electron transport, cytochrome c acts as an apoptosis-triggering agent, forming the apoptosome with apoptosis protease-activating factor-1 (Apaf-1) and activating the caspase cascade. The two functions are finely tuned by phosphorylation of Tyr residues at position 48 and/or 97. One recent report provides suggestive evidence that phosphorylation at Tyr48 switches off the apoptotic effect.
In addition to its function in electron transport, cytochrome c acts as an apoptosis-triggering agent, forming the apoptosome with apoptosis protease-activating factor-1 (Apaf-1) and activating the caspase cascade. The two functions are finely tuned by phosphorylation of Tyr residues at position 48 and/or 97. One recent report provides suggestive evidence that phosphorylation at Tyr48 switches off the apoptotic effect.
Postać fizyczna
Lyophilized from a 0.2 μm filtered solution in phosphate buffered saline.
Uwaga dotycząca przygotowania
Chemically oxidized during purification.
Inne uwagi
View more information on cytochrome c and electron transport at www.sigma-aldrich.com/enzymeexplorer.
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Zastosowanie
Kod klasy składowania
13 - Non Combustible Solids
Klasa zagrożenia wodnego (WGK)
WGK 3
Temperatura zapłonu (°F)
Not applicable
Temperatura zapłonu (°C)
Not applicable
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Produkty
Learn about the four membrane-bound protein complexes that make up the electron transport chain metabolic pathway supplying energy as ATP for cellular respiration.
Chromatograms
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