C1117
Anti-Cy5 antibody, Mouse monoclonal
clone CY5-15, purified from hybridoma cell culture
Synonim(y):
Przeciwciało Cy5, Przeciwciało Cy5 - Przeciwciało anty-Cy5, mysie monoklonalne
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About This Item
Polecane produkty
pochodzenie biologiczne
mouse
Poziom jakości
białko sprzężone
unconjugated
forma przeciwciała
purified immunoglobulin
rodzaj przeciwciała
primary antibodies
klon
CY5-15, monoclonal
Formularz
buffered aqueous solution
opakowanie
antibody small pack of 25 μL
stężenie
~1.5 mg/mL
metody
direct ELISA: suitable
dot blot: 1-2 μg/mL using cell protein extracts labeled with Cy5
immunocytochemistry: suitable
immunoprecipitation (IP): suitable
microarray: suitable
izotyp
IgG1
Warunki transportu
dry ice
temp. przechowywania
−20°C
docelowa modyfikacja potranslacyjna
unmodified
Opis ogólny
CyDyes are a family of fluorophores that can be used for labeling proteins, peptides, DNA, RNA, and other biomolecules. These dyes are small, pH insensitive (between pH 3 and pH 10), and soluble in aqueous solution and have tolerance to DMSO. They are more photo-stable than fluorescein, have high molar extinction coefficients, and favorable quantum yields. Due to their physical and chemical properties, they are used (mainly Cy3 and Cy5) in many different biological assays such as: DNA microarrays, protein microarrays, two-dimensional protein analysis (2D gels), fluorescence resonance energy transfer (FRET), and immunocytochemistry.
Mouse monoclonal clone CY5-15 anti-Cy5 antibody recognizes Cy5 and AlexaFluor647 labeled proteins, but not Cy3 labeled proteins.
Specyficzność
Monoclonal Anti-Cy5 recognizes Cy5 and AlexaFluor647 labeled proteins, but not Cy3 labeled proteins.
The antibody recognizes Cy5 and AlexaFluor 647 labeled proteins, but not Cy3 labeled proteins.
Immunogen
mixture of proteins labeled with Cy5.
Zastosowanie
Anti-Cy5 antibody, Mouse monoclonal has been used in immunofluorescence assays and western blotting.
Mouse monoclonal clone CY5-15 anti-Cy5 antibody is used to tag CyDye 5 for detection and quantitation and signal applification by or within immunocytochemical and immunohistochemical (IHC) techniques such as ELISA, immunoprecipitation, immunocytochemistry, dot blot assay, and protein microarrays. Anti-Cy5 antibody is an important tool for Cy5 signal amplification in assays where biomolecules are labeled with Cy5.
Postać fizyczna
Solution in 0.01 M phosphate buffered saline, pH 7.4, containing 15 mM sodium azide.
Oświadczenie o zrzeczeniu się odpowiedzialności
Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.
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Kod klasy składowania
12 - Non Combustible Liquids
Klasa zagrożenia wodnego (WGK)
WGK 1
Temperatura zapłonu (°F)
Not applicable
Temperatura zapłonu (°C)
Not applicable
Środki ochrony indywidualnej
Eyeshields, Gloves, multi-purpose combination respirator cartridge (US)
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