Key Documents
AL0103000
ALiCE® Cell Free Protein Expression Kit (Plant Cell Lysate)
An eukaryotic cell free expression system with unprecedented 3 mg/mL recombinant protein yield in batch mode and post-translational modifications enabled
Synonim(y):
Cell-Free Expression Kit
About This Item
Polecane produkty
Postać
liquid (aqueous solutions)
opakowanie
pkg of 6 × 200 μL (AL0103200 (ALiCE® Midi Kit))
pkg of 6 × 50 μL (AL0103050 (ALiCE® Mini Kit))
pkg of 6 × 500 μL (AL0103500 (ALiCE® Maxi Kit))
metody
protein expression: suitable
Warunki transportu
dry ice
temp. przechowywania
−70°C
Opis ogólny
To address this problem, ALiCE® a novel eukaryotic cell-free expression system was created by taking advantage of plant cell biology. ALiCE® offers unprecedented yields of up to 3 mg/mL of protein in batch mode. As such, it enables you to overcome the limitations of living host systems and to obtain “difficult to produce” proteins in a matter of hours instead of weeks.
ALiCE® utilizes tobacco cell lysates, depleted of vacuoles. The system contains all factors required for in vitro transcription (RNA polymerases, NTPs) and translation reactions (ribosomes, translation initiation/elongation factors, tRNA, etc.). Using a simple protocol, both the RNA transcription and translation take place in a single tube (coupled transcription/translation).
Reactions are started by simply mixing plasmid DNA with the ALiCE® Reaction mix. The outstanding protein yields of ALiCE® are based on highly efficient protein expression over the duration of the reaction. Consequently, we recommend a reaction duration of 48 h.
ALiCE® is provided with two expression vectors, pALiCE01 and pALiCE02, which are used according to the targeting of the protein of interest. These have been specially tailored to offer excellent protein yield when used with the ALiCE® expression system. pALiCE01 is used for expression in ALiCE® cytosolic fraction. Targeting the microsomes with pALiCE02 offers the opportunity to express protein with post-translational modifications like disulfide bonds.
Zastosowanie
- Target protein characterization
- Protein optimization
- Mutant screening
- Protein-protein interactions
- Expression analysis
- Protein localization analysis
- Protein structure analysis
- Post-translational modification assays
- Metabolomics
- Herbicide screening
Cechy i korzyści
- High-yield: Up to 3 mg/mL expressed protein
- Easy to use: One-step reaction protocol
- Fast: High yield in just 48 hours
- Flexible: Express “difficult to produce” proteins, cytotoxic proteins, etc.
Komponenty
ALiCE® Lysate, 6 x 50 μL
Vector pALiCE01, 15 μL
Vector pALiCE02, 15 μL
ALiCE® Tube Cap, 0.6 mm hole
ALiCE® Midi Kit - AL0103200 Comprises
ALiCE® Lysate, 6 x 200 μL
Vector pALiCE01, 50 μL
Vector pALiCE02, 50 μL
ALiCE® Tube Set, 12 pcs
ALiCE® Maxi Kit - AL0103500 Comprises
ALiCE® Lysate, 6 x 500 μL
Vector pALiCE01, 50 μL
Vector pALiCE02, 50 μL
ALiCE® Tube Set, 12 pcs
Przechowywanie i stabilność
- ALiCE® Reaction (Lysate) mix: store at -70 °C
- Vector pALiCE01: store at -20 °C to -70 °C
- Vector pALiCE02: store at -20 °C to -70 °C
- ALiCE® Tube caps/Tube set: store at room temperature
Informacje prawne
Kod klasy składowania
10 - Combustible liquids
Klasa zagrożenia wodnego (WGK)
WGK 3
Certyfikaty analizy (CoA)
Poszukaj Certyfikaty analizy (CoA), wpisując numer partii/serii produktów. Numery serii i partii można znaleźć na etykiecie produktu po słowach „seria” lub „partia”.
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Produkty
A form of synthetic biology, cell-free protein synthesis or expression (CFPE) is a rapid method to produce recombinant proteins in solution without involving cells. Using biomolecular translation machinery extracted from a range of different cell types, researchers can express a protein of interest within a day.
Bezkomórkowa synteza białek szybko wytwarza rekombinowane białka bez udziału komórek, wykorzystując do tego celu maszyny translacyjne z różnych typów komórek.
Protokoły
Achieve high protein yields with the ALiCE® Cell-Free Protein Synthesis System that allows you to obtain “difficult to produce” proteins in a matter of hours instead of weeks.
Follow these five quick steps to perform cell-free protein synthesis using the ALiCE® protein expression plant lysate kit.
Powiązane treści
Prosimy o zapoznanie się z treścią ograniczonej licencji, która szczegółowo opisuje dozwolony sposób wykorzystywania systemu do bezkomórkowej ekspresji białek ALiCE™ wyłącznie do celów badań in vitro.
Nasz zespół naukowców ma doświadczenie we wszystkich obszarach badań, w tym w naukach przyrodniczych, materiałoznawstwie, syntezie chemicznej, chromatografii, analityce i wielu innych dziedzinach.
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