A8706
Avidin from egg white
recombinant, expressed in corn, ≥12 units/mg protein (E1%/280)
Synonim(y):
AVID_CHICK
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About This Item
Polecane produkty
rekombinowane
expressed in corn
Próba
≥80% protein basis (biuret)
Formularz
powder
aktywność właściwa
≥12 units/mg protein (E1%/280)
masa cząsteczkowa
glycoprotein 66 kDa
subunit 16 kDa
metody
ELISA: suitable
immunoblotting: suitable
immunoelectrophoresis: suitable
numer dostępu UniProt
temp. przechowywania
2-8°C
informacje o genach
chicken ... AVD(396260)
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Opis ogólny
Avidin is a homotetrameric glycoprotein found in the egg white of birds, reptiles and amphibians. Each subunit is 16 kDa, singly glycosylated and binds to a molecule of biotin with greater affinity and specificity. Recombinant avidin from corn is similar to avidin from egg white in terms of properties like isoelectric point (pI) and antigenic properties except for the molecular weight. Avidin from corn has low molecular weight than chicken egg-derived avidin. This might be due to less complex glycosylation composition in plants. Commercial production of avidin from corn has certain advantages in terms of availability of greater biomass and avoiding the co-purification of animal virus.
Zastosowanie
Avidin egg white was used in an assay using functionalized xenon as a biosensor to detect biotin-avidin binding. Egg white was used at 80 nmol.
Avidin forms an extremely strong complex with d-biotin (Kd ~ 10-15). This activity has made both avidin and biotin extremely useful labels in immunochemical methods of detection and quantitation.
Definicja jednostki
One unit will bind 1.0 μg of d-biotin.
Uwaga dotycząca przygotowania
Affinity purified
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Kod klasy składowania
11 - Combustible Solids
Klasa zagrożenia wodnego (WGK)
WGK 3
Temperatura zapłonu (°F)
Not applicable
Temperatura zapłonu (°C)
Not applicable
Środki ochrony indywidualnej
Eyeshields, Gloves, type N95 (US)
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