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A7419
Avidin–Peroxidase
buffered aqueous solution
Synonim(y):
Avidin–HRP
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About This Item
Polecane produkty
pochodzenie biologiczne
avidin from egg white
enzyme from horseradish
Poziom jakości
białko sprzężone
peroxidase conjugate
Postać
buffered aqueous solution
metody
indirect ELISA: 1:100,000
western blot: 1:400,000-1:800,000 using detecting β-Actin in total cell extract of HeLa cells (5-10 μg/well)
Warunki transportu
dry ice
temp. przechowywania
−20°C
Opis ogólny
Avidin is homotetrameric protein (68kDa) obtained from egg whites and binds strongly to biotin. Thus, avidin-biotin association has been utilized in immunoassays to detect the localization of antigens in tissues. The use of avidin-biotin immunoassay enhances the sensitivity of the technique and facilitates the detection of antigens in low quantities.
Zastosowanie
Avidin-Peroxidase has been used for ELISA applications. The product can also be used for western blot applications at dilutions ranging from 1:400,000 to 1:800,000.
Western blot analysis of proteins in HUVEC cells that had underwent cell surface biotinylation were detected using HRP-conjugated avidin at 33.3 ng/ml.
Postać fizyczna
Solution in 0.01 M phosphate buffered saline, pH 7.4, containing 0.05% MIT.
Uwaga dotycząca przygotowania
Affinity purified protein.
Oświadczenie o zrzeczeniu się odpowiedzialności
Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.
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Kod klasy składowania
12 - Non Combustible Liquids
Klasa zagrożenia wodnego (WGK)
WGK 2
Temperatura zapłonu (°F)
Not applicable
Temperatura zapłonu (°C)
Not applicable
Certyfikaty analizy (CoA)
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