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Key Documents
A7080
Amphiregulin human
≥97% (SDS-PAGE), recombinant, expressed in E. coli, lyophilized powder, suitable for cell culture
Synonim(y):
AREG, CRDGF, SDGF, AR
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About This Item
Polecane produkty
product name
Amphiregulin human, recombinant, expressed in E. coli, lyophilized powder, suitable for cell culture, ≥97% (SDS-PAGE)
pochodzenie biologiczne
human
Poziom jakości
rekombinowane
expressed in E. coli
Próba
≥97% (SDS-PAGE)
Postać
lyophilized powder
siła działania
10-100 ng/mL ED50/EC50
masa cząsteczkowa
~11 kDa
opakowanie
pkg of 1 mg
warunki przechowywania
avoid repeated freeze/thaw cycles (Do not store in a frost-free freezer.)
metody
cell culture | mammalian: suitable
zanieczyszczenia
endotoxin, tested
numer dostępu UniProt
temp. przechowywania
−20°C
informacje o genach
human ... AREG(374)
Działania biochem./fizjol.
Also known as keratinocyte autocrine factor (KAF); expression of amphiregulin mRNA has been found in numerous carcinoma cell lines and epithelial cells of several human tissues including colon, stomach, breast, ovary, and kidney.
Komponenty
Human amphiregulin is produced by a DNA sequence that encodes a 98 amino acid residue form of mature human amphiregulin. This corresponds to amino acid residues 101-198 in E. coli. Amphiregulin is a glycoprotein produced in response to treatment of the cell line MCF-7, a human breast carcinoma cell line, with PMA, a protein kinase C activator and potent tumor promoter.
Przestroga
This product should be stored at -20°C. After preparation, the product can be stored at 2-8°C for one month, or frozen in aliquots at -20°C or -70°C for more extended storage.
Postać fizyczna
Lyophilized from a 0.2 μm filtered solution in PBS, pH 7.4 with 50 μg BSA per 1 μg as a carrier protein.
Uwaga dotycząca przygotowania
It should be reconstituted by adding sterile PBS containing 0.1% HAS or BSA to a concentration greater than 10 μg/mL.
Komentarz do analizy
Measured in a cell proliferation assay using Balb/3T3 mouse embryonic fibroblast cells. Marquardt, H. et al. (1984) Science 223:1079. The ED50 for this effect is typically 5-15 ng/mL.
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Kod klasy składowania
11 - Combustible Solids
Klasa zagrożenia wodnego (WGK)
WGK 3
Temperatura zapłonu (°F)
Not applicable
Temperatura zapłonu (°C)
Not applicable
Środki ochrony indywidualnej
Eyeshields, Gloves, type N95 (US)
Certyfikaty analizy (CoA)
Poszukaj Certyfikaty analizy (CoA), wpisując numer partii/serii produktów. Numery serii i partii można znaleźć na etykiecie produktu po słowach „seria” lub „partia”.
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