A6255

α-Amylase from porcine pancreas

PMSF Treated, Type I-A, saline suspension, ≥1000 units/mg protein (E1%/280)

• Synonim(y): β-N-acetylglucosaminidase porcine placenta, PPA, al1,4 glucan-4-glucanohydrolase,, porcine pancreas α-amylase
• Numer EC enzymu: 3.2.1.1 (BRENDA, IUBMB)
• Numer WE: 232-565-6
• Numer MDL: MFCD00081319
• Kod UNSPSC: 12352204
• eCl@ss: 32160410
• NACRES: NA.54

Właściwości

• pochodzenie biologiczne: Porcine pancreas
• Poziom jakości: 200
• typ: Type I-A
• Formularz: saline suspension
• aktywność właściwa: ≥1000 units/mg protein (E1%/280)
• masa cząsteczkowa: 51-54 kDa
• charakterystyka ekologicznej alternatywy: Waste Prevention; Design for Energy Efficiency; Learn more about the Principles of Green Chemistry.
• sustainability: Greener Alternative Product
• kategoria ekologicznej alternatywy: , Enabling
• Warunki transportu: wet ice
• temp. przechowywania: 2-8°C

Opis

Opis ogólny

Molecular mass: 51-54 kDa.
α-Amylase isolated from porcine pancreas is a glycoprotein. It is a single polypeptide chain of ~475 residues containing two SH groups and four disulfide bridges and a tightly bound Ca²⁺ necessary for stability. Chloride ions are necessary for activity and stability. The pH range for activity is 5.5 to 8.0, with the pH optimum at 7.

We are committed to bringing you Greener Alternative Products, which adhere to one or more of The 12 Principles of Greener Chemistry. This product has been enhanced for energy efficiency and waste prevention when used in starch ethanol research. For more information see the article in biofiles.

Zastosowanie

α-Amylase is used to hydrolyze α bonds of α-linked polysaccharides, such as starch and glycogen. Product A6255 is from porcine pancreas and is type I-A. α-Amylase, from Sigma, has been used in various plant studies, such as metabolism studies in Arabidopsis .

Działania biochem./fizjol.

α-Amylase hydrolyzes the α-(1,4) glucan linkages in polysaccharides of three or more α-(1,4) linked D-glucose units. Natural substrates such as starch and glycogen are broken down into glucose and maltose. α -Amylase, from porcine pancreas, is a glycoprotein that consists of a single polypeptide chain of approximately 475 residues containing 2 SH groups and four disulfide bridges and a tightly bound Ca²⁺ necessary for stability.

Definicja jednostki

One unit will liberate 1.0 mg of maltose from starch in 3 min at pH 6.9 at 20 °C.

Postać fizyczna

Suspension in 2.9 M NaCl solution containing 3 mM CaCl₂

Uwaga dotycząca przygotowania

PMSF treated. 2× crystallized

Inne uwagi

View more information on enzymes for complex carbohydrate analysis at www.sigma-aldrich.com/enzymeexplorer

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Informacja o środkach bezpieczeństwa

• Piktogramy: GHS08
• Hasło ostrzegawcze: Danger
• Zwroty wskazujące rodzaj zagrożenia: H334
• Zwroty wskazujące środki ostrożności: P261 - P284 - P501
• Klasyfikacja zagrożeń: Resp. Sens. 1
• Kod klasy składowania: 11 - Combustible Solids
• Klasa zagrożenia wodnego (WGK): WGK 1
• Temperatura zapłonu (°F): Not applicable
• Temperatura zapłonu (°C): Not applicable
• Środki ochrony indywidualnej: dust mask type N95 (US), Eyeshields, Faceshields, Gloves