A4312

Anti-Mouse IgG (whole molecule)−Alkaline Phosphatase antibody produced in rabbit

affinity isolated antibody, buffered aqueous glycerol solution

• Synonim(y): Rabbit Anti-Mouse IgG (whole molecule)−AP
• Numer MDL: MFCD00162640
• Kod UNSPSC: 12352203
• NACRES: NA.46

Właściwości

• pochodzenie biologiczne: rabbit
• białko sprzężone: alkaline phosphatase conjugate
• forma przeciwciała: affinity isolated antibody
• rodzaj przeciwciała: secondary antibodies
• klon: polyclonal
• Postać: buffered aqueous glycerol solution
• reaktywność gatunkowa: mouse
• metody: direct ELISA: 1:30,000; immunohistochemistry (formalin-fixed, paraffin-embedded sections): 1:50; western blot: 1:30,000 using using β-Actin in total cell extract of HeLa cells (5-10 μg/well)
• Warunki transportu: wet ice
• temp. przechowywania: 2-8°C
• docelowa modyfikacja potranslacyjna: unmodified

Opis

Opis ogólny

IgG antibodies are the smallest antibody isotype found in blood and extracellular fluid, capable of fighting bacterial and viral infection.

Immunoglobulin G (IgG) belongs to the immunoglobulin family and is a widely expressed serum antibody. An immunoglobulin has two heavy chains and two light chains connected by a disulfide bond. It mainly helps in immune defense. It is a glycoprotein. IgG is a major class of immunoglobulin. Mouse consists of five immunoglobulin classes- IgM, IgG, IgA, IgD and IgE. Mouse IgG is further divided into five classes- IgG1, IgG2a, IgG2b and IgG3.

Immunogen

Purified mouse IgG.

Zastosowanie

Anti-Mouse IgG (whole molecule) Alkaline Phosphatase antibody produced in rabbit has been used in immunocytochemical localization of Aβ peptides and western blot analysis.

Anti-mouse IgG (whole molecule)-alkaline phosphatase antibody can be used in immunocytochemistry.

Western blot analysis of lysates from the placental cell line 3ASubE-P-3 and U037 cells were performed using alkaline phosphatase conjugated rabbit anti- mouse IgG as the secondary at 1:2000 to recognize the mouse mom Il-8RB. Secondary was developed using bromochloroindoyl phosphate (Sigma) pH. 9.5 for 10-15 minutes.Western blot analysis of coimmunoprecipitates from human cell or rat cell vaginal extracts were performed using alkaline phosphatase conjugated rabbit anti- mouse IgG as the secondary at 1:15000 dilution.

Działania biochem./fizjol.

Immunoglobulin G (IgG) participates in hypersensitivity type II and type III reactions. IgG helps in opsonization, complement fixation and antibody dependent cell mediated cytotoxicity.

Postać fizyczna

Solution in 0.05 M Tris buffer, pH 8.0, containing 1 mM MgCl₂, 10 mM glycine, 1% bovine serum albumin, 50% glycerol and 15 mM sodium azide.

Oświadczenie o zrzeczeniu się odpowiedzialności

Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.

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Informacja o środkach bezpieczeństwa

• Kod klasy składowania: 10 - Combustible liquids
• Klasa zagrożenia wodnego (WGK): WGK 3
• Temperatura zapłonu (°F): Not applicable
• Temperatura zapłonu (°C): Not applicable