A2917

Acrylamide/Bis-acrylamide

for molecular biology, BioReagent, 19:1 (ratio)

• Synonim(y): Acrylamide/Bisacrylamide, 19:1 (5% crosslinker), Polyacrylamide solution
• Numer MDL: MFCD00080848
• Kod UNSPSC: 41105319
• Identyfikator substancji w PubChem: 329770804
• NACRES: NA.25

Właściwości

• klasa czystości: for molecular biology
• Poziom jakości: 200
• linia produktu: BioReagent
• Postać: powder
• proporcje: 19:1 (ratio)
• obecność zanieczyszczeń: DNAse (exonuclease), NICKase (endonuclease), RNAse, and protease, none detected
• ciąg SMILES: NC(=O)C=C.C=CC(=O)NCNC(=O)C=C
• InChI: 1S/C7H10N2O2.C3H5NO/c1-3-6(10)8-5-9-7(11)4-2;1-2-3(4)5/h3-4H,1-2,5H2,(H,8,10)(H,9,11);2H,1H2,(H2,4,5)
• Klucz InChI: OCQZXMCGTAWGEQ-UHFFFAOYSA-N

Opis

Opis ogólny

Polyacrylamide gels are prepared using acrylamide and bis-acrylamide, where bis-acrylamide is a cross-linking agent. On the other hand, acrylamide is a water soluble monomer which helps in forming a transparent stable insoluble gel.

Zastosowanie

Acrylamide/Bis-acrylamide has been used:

• in the preparation of urea polyacrylamide gel during a modified cross-linking affinity purification protocol (cCLAP)
• in microsatellite genotyping and nonfluorescent elastin (ELN) (GT)n polymorphism genotyping
• in polyacrylamide gel for separation of proteins in SDS-PAGE for western blotting

Ready to reconstitute dry powder blends are accurately pre-blended to produce a 40% (w/v) stock solution for use in protein and nucleic acid electrophoresis. The concentration is based on the total weight of both the acrylamide and bis-acrylamide.

Cechy i korzyści

Eliminates the need to weigh toxic acrylamide and bis-acrylamide.

Rekonstytucja

Reconstitute by slowly adding the indicated amount of distilled water to the container and dissolve contents.

To make up 100 ml of A2917, slowly add 63 ml distilled or deionized water to the container and dissolve contents. Do not divide the solid. This makes 100 ml of a 40% stock solution.
For the 1 Liter size, use 630 mL of water.

This page may contain text that has been machine translated.

Informacja o środkach bezpieczeństwa

• Piktogramy: GHS06,GHS08
• Hasło ostrzegawcze: Danger
• Zwroty wskazujące rodzaj zagrożenia: H301,H312 + H332,H315,H317,H319,H340,H350,H361fd,H372
• Zwroty wskazujące środki ostrożności: P202 - P280 - P301 + P310 - P302 + P352 + P312 - P304 + P340 + P312 - P305 + P351 + P338
• Klasyfikacja zagrożeń: Acute Tox. 3 Oral - Acute Tox. 4 Dermal - Acute Tox. 4 Inhalation - Carc. 1B - Eye Irrit. 2 - Muta. 1B - Repr. 2 - Skin Irrit. 2 - Skin Sens. 1 - STOT RE 1 Oral
• Organy docelowe: Peripheral nervous system
• Kod klasy składowania: 6.1C - Combustible acute toxic Cat.3 / toxic compounds or compounds which causing chronic effects
• Klasa zagrożenia wodnego (WGK): WGK 3
• Temperatura zapłonu (°F): Not applicable
• Temperatura zapłonu (°C): Not applicable
• Środki ochrony indywidualnej: Eyeshields, Faceshields, Gloves, type P2 (EN 143) respirator cartridges