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P9620

Sigma-Aldrich

Puromycin dihydrochloride

Ready Made Solution, from Streptomyces alboniger, 10 mg/mL in H2O, suitable for cell culture

Synonim(y):

3′-(L-α-Amino-p-methoxyhydrocinnamamido)-3′-deoxy-N,N-dimethyladenosine dihydrochloride, Stylomycin dihydrochloride

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About This Item

Numer CAS:
58-58-2
Numer MDL:
MFCD00012691
Kod UNSPSC:
51281906
Identyfikator substancji w PubChem:
329820352
NACRES:
NA.85

pochodzenie biologiczne

Streptomyces alboniger

Poziom jakości

300

typ

for cell culture

Próba

>98% (HPLC)

Postać

solution

warunki przechowywania

(Store in cool place. Keep container tightly closed in a dry and well -ventilated place. )

stężenie

10 mg/mL in H2O

metody

cell culture | mammalian: suitable

kolor

colorless to yellow

rozpuszczalność

H2O: soluble 10 mg/mL

spektrum działania antybiotyku

Gram-positive bacteria
neoplastics
parasites

Tryb działania

protein synthesis | interferes

Warunki transportu

wet ice

temp. przechowywania

−20°C

ciąg SMILES

Cl.Cl.COc1ccc(C[C@H](N)C(=O)N[C@H]2[C@@H](O)[C@@H](O[C@@H]2CO)n3cnc4c(ncnc34)N(C)C)cc1

InChI

1S/C22H29N7O5.2ClH/c1-28(2)19-17-20(25-10-24-19)29(11-26-17)22-18(31)16(15(9-30)34-22)27-21(32)14(23)8-12-4-6-13(33-3)7-5-12;;/h4-7,10-11,14-16,18,22,30-31H,8-9,23H2,1-3H3,(H,27,32);2*1H/t14-,15+,16+,18+,22+;;/m0../s1

Klucz InChI

MKSVFGKWZLUTTO-FZFAUISWSA-N

Opis ogólny

Puromycin, also known as clomycin or stylomycin hydrochloride, is an aminonuclease antibiotic and protein synthesis inhibitor. It is produced by the soil actinomycete bacterium Streptomyces alboniger through a series of enzymatic reactions. Puromycin is effective against both prokaryotic and eukaryotic cells, working by interfering with RNA function, leading to the inhibition of protein synthesis. It also serves as an inhibitor of aminopeptidase and enkephalinase. The growth of bacteria, protozoa, algae, and mammalian cells is quickly halted by puromycin, but cells with the pac gene exhibit resistance. The gene product of the pac gene is puromycin-N-acetyl-transferase, which inactivates puromycin through acetylation.

Puromycin′s structural similarity to the amino acid-bearing end of tRNA allows it to enter the ribosome during protein synthesis, bind to the nascent polypeptide chain, and halt chain elongation. Widely used in cell culture, puromycin serves as a selective agent for cells that have been transformed with a gene conferring resistance to puromycin.

Zastosowanie

Puromycin is an aminonucleoside antibiotic that is derived from Streptomyces alboniger. It allows selection for cells that contain the resistance gene puromycin N-acetyl-transferase (PAC). It has been used to study vascular smooth muscle cell viability after treatment in the rabbit model . Puromycin is used to produce enhanced green fluorescent protein (EGFP) transgenic piglets after somatic cell cloning and embryo transfer.

Działania biochem./fizjol.

Mode of Action: Puromycin inhibits protein synthesis by causing premature chain termination acting as an analog of the 3′-terminal end of the aminoacyl-tRNA. Puromycin also acts as a reversible inhibitor of dipeptidyl-peptidase II (serine peptidase) and cytosol alanyl aminopeptidase.

Mode of Resistance: Puromycin acetyltransferase is an effective resistance gene.

Antimicrobial Spectrum: This product is active against gram-positive microorganisms, less active against acid-fast bacilli and more weakly active against gram-negative microorganisms. Puromycin can prevent growth of bacteria, protozoa, algae and mammalian cells and acts quickly, killing 99% of cells within 2 days.

Cechy i korzyści

  • High-quality antibiotic suitable for multiple research applications
  • Antibiotic and protein synthesis inhibitor
  • Effective against prokaryotic and eukaryotic cells
  • Inhibits protein synthesis by interfering with RNA function
  • Commonly used as a selective agent in cell biology research

Uwaga dotycząca przygotowania

This product is concentrated at 10 mg/mL in water. It has been tested on HeLa cells for cell growth arrest and selection of cells after transfection of the pac resistance gene.

Inne uwagi

For additional information on our range of Biochemicals, please complete this form.
Keep container tightly closed in a dry and well-ventilated place.
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produkt podobny

Numer produktu
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Kod klasy składowania

10 - Combustible liquids

Klasa zagrożenia wodnego (WGK)

WGK 2

Temperatura zapłonu (°F)

Not applicable

Temperatura zapłonu (°C)

Not applicable

Środki ochrony indywidualnej

Eyeshields, Faceshields, Gloves, multi-purpose combination respirator cartridge (US)

Certyfikaty analizy (CoA)

Poszukaj Certyfikaty analizy (CoA), wpisując numer partii/serii produktów. Numery serii i partii można znaleźć na etykiecie produktu po słowach „seria” lub „partia”.

Masz już ten produkt?

Dokumenty związane z niedawno zakupionymi produktami zostały zamieszczone w Bibliotece dokumentów.

Odwiedź Bibliotekę dokumentów

Satoshi Watanabe et al.
Biology of reproduction, 72(2), 309-315 (2004-09-24)
Puromycin N-acetyl transferase gene (pac), of which the gene product catalyzes antibiotic puromycin (an effective inhibitor of protein synthesis), has been widely used as a dominant selection marker in embryonic stem (ES) cell-mediated transgenesis. The present study is the first
Jacinta P W Lee et al.
Autophagy, 12(6), 907-916 (2016-05-11)
MIF (macrophage migration inhibitory factor [glycosylation-inhibiting factor]) is a pro-inflammatory cytokine expressed in multiple cells types, including macrophages. MIF plays a pathogenic role in a number of inflammatory diseases and has been linked to tumor progression in some cancers. Previous
Ming Lee Lin et al.
Proceedings of the National Academy of Sciences of the United States of America, 105(8), 3029-3034 (2008-02-15)
Cross-presentation as a fundamental pathway of activating CD8(+) T cells has been well established. So far the application of this concept in vivo is limited, and the mechanisms that specialize CD8(+) dendritic cells (DCs) for this task are not fully
S Eyckerman et al.
Nature cell biology, 3(12), 1114-1119 (2002-01-10)
Ligand-induced clustering of type I cytokine receptor subunits leads to trans-phosphorylation and activation of associated cytosolic janus kinases (JAKs). In turn, JAKs phosphorylate tyrosine residues in the receptor tails, leading to recruitment and activation of signalling molecules. Among these, signal
Valerie Croons et al.
The Journal of pharmacology and experimental therapeutics, 325(3), 824-832 (2008-03-07)
Recent evidence indicates that the protein synthesis inhibitor cycloheximide triggers selective macrophage death in rabbit atheroma-like lesions without affecting smooth muscle cells (SMCs) or the endothelium, thereby favoring a stable plaque phenotype. In this study, we report that puromycin, a
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