Przejdź do zawartości
Merck
Wszystkie zdjęcia(3)

Key Documents

View All Documentation

KCQS00

Sigma-Aldrich

KiCqStart® SYBR® Green qPCR ReadyMix™

For Bio-Rad, Cepheid, Eppendorf, Illumina, Corbett, and Roche systems

Synonim(y):

qPCR master mix, sybr green qPCR

Zaloguj sięWyświetlanie cen organizacyjnych i kontraktowych
Wszystkie zdjęcia(3)

About This Item

Kod UNSPSC:
41106300
NACRES:
NA.55

Postać

liquid

zastosowanie

sufficient for 1250 reactions
 sufficient for 250 reactions
 sufficient for 5000 reactions

Właściwości

dNTPs included
hotstart

warunki przechowywania

protect from light

metody

qPCR: suitable

kolor

colorless

moc wejściowa

purified DNA

kompatybilność

for use with Bio-Rad CFX384
for use with Bio-Rad CFX96
for use with Bio-Rad MJ Chromo4
for use with Bio-Rad MJ Opticon 2
for use with Bio-Rad MJ Opticon Cepheid SmartCycler
for use with Bio-Rad MiniOpticon
for use with Bio-Rad MyiQ
for use with Eppendorf® Mastercycler ep realplex2 s
for use with Eppendorf® Mastercycler ep realplex
for use with Illumina Eco qPCR
for use with Qiagen Corbett Rotor-Gene 3000
for use with Qiagen Corbett Rotor-Gene 6000
for use with Qiagen Corbett Rotor-Gene Q
for use with Roche LightCycler 480

metoda wykrywania

SYBR® Green

Warunki transportu

dry ice

temp. przechowywania

−20°C

Szukasz podobnych produktów? Odwiedź Przewodnik dotyczący porównywania produktów

Opis ogólny

KiCqStart SYBR Green qPCR ReadyMix is a 2X concentrated, ready-to-use master mix that contains all components, except primers and template, for real-time quantitative PCR (qPCR) This unique combination of proprietary buffer, stabilizers, and Hot-Start Taq DNA polymerase delivers maximum PCR efficiency, sensitivity, specificity and robust fluorescent signal using fast, or conventional, cycling protocols with SYBR Green qPCR.

Highly specific amplification is crucial to successful qPCR with SYBR Green I dye technology because this dye binds to and detects any dsDNA generated during amplification. Hot-Start Taq DNA polymerase is antibody mediated to be inactive prior to the initial PCR denaturation step.

Zastosowanie

KiCqStart® SYBR® Green qPCR ReadyMix™ has been used to perform quantitative real-time PCR (qPCR) for the amplification and quantification of genomic DNA extracted from human gut bacterium Prevotella copri.
PCR applications:
  • Gene expression
  • DNA quantification
  • CHiP

Cechy i korzyści

  • Assay results in as little as 33 minutes
  • Highly efficient and sensitive real-time PCR results
  • Little/no optimization required

Komponenty

2X reaction buffer containing optimized concentrations of MgCl2, dNTPs (dATP, dCTP, dGTP, dTTP), KiCqStart Taq DNA Polymerase, SYBR Green dye, and stabilizers

packaging:
250 reactions* = 2 X 1.25 mL tubes
1250 reactions* = 10 X 1.25 mL tubes
5000 reactions* = 1 X 50 mL tube
*number of reactions based on a 20uL volume

Inne uwagi

Storage Conditions:
KiCqStart SYBR Green qPCR ReadyMix is stable for 1 year when stored in a constant temperature freezer at -20°C, protected from light. For convenience, it may be stored unfrozen at +2 to +8°C for up to 6 months. After thawing, mix thoroughly before using. Repeated freezing and thawing of the product is not recommended. However, the product demonstrated no loss of performance after 20 freeze-thaw cycles or 2 months at +20°C.
Instrument Compatibility:
Different real-time PCR systems employ different strategies for normalization of fluorescent signals and correction of well-to-well optical variations. It is critical to match the appropriate qPCR reagent to your specific instrument. KiCqStart SYBR Green qPCR ReadyMix does not contain an internal reference dye. Compatible instruments include:
  • Bio-Rad® CFX384™
  • Bio-Rad CFX96™
  • Bio-Rad MiniOpticon™
  • Bio-Rad/MJ Chromo4™
  • Bio-Rad/MJ Opticon 2
  • Bio-Rad/MJ Opticon™
  • Cepheid SmartCycler®
  • Eppendorf Mastercycler® ep realplex
  • Eppendorf Mastercycler ep realplex2 s
  • Illumina Eco qPCR
  • Qiagen/Corbett Rotor-Gene® 3000
  • Qiagen/Corbett Rotor-Gene 6000
  • Qiagen/Corbett Rotor-Gene Q
  • Roche LightCycler® 480

Informacje prawne

Bio-Rad is a registered trademark of Bio-Rad Laboratories, Inc.
CFX384 is a trademark of Bio-Rad Laboratories, Inc.
CFX96 is a trademark of Bio-Rad Laboratories, Inc.
Chromo4 is a trademark of Bio-Rad Laboratories, Inc.
Eppendorf is a registered trademark of Eppendorf AG
KiCqStart is a registered trademark of QIAGEN Beverly Inc.
LightCycler is a registered trademark of Roche
Mastercycler is a registered trademark of Eppendorf AG
MiniOpticon is a trademark of Bio-Rad Laboratories, Inc.
Opticon is a trademark of Bio-Rad Laboratories, Inc.
ReadyMix is a trademark of Sigma-Aldrich Co. LLC
Rotor-Gene is a registered trademark of Qiagen GmbH
SYBR is a registered trademark of Life Technologies
SmartCycler is a registered trademark of Cepheid, Inc.
This page may contain text that has been machine translated.

polecane

Numer produktu
Opis
Cennik

produkt powiązany

Numer produktu
Opis
Cennik

S4438

SYBR® Green JumpStart™ Taq ReadyMix™, for quantitative PCR, MgCI2 in buffer

S1816

SYBR® Green JumpStart™ Taq ReadyMix™ for Quantitative PCR, Capillary Formulation, SYBR® Green qPCR reagent for Roche LightCycler® capillary systems

S5193

SYBR® Green JumpStart™ Taq ReadyMix™, for quantitative PCR, without MgCl2 in buffer

S9194

SYBR® Green JumpStart™ Taq ReadyMix™ for High Throughput qPCR, SYBR® Green qPCR reagent, passive reference dye included

L6544

LuminoCt® SYBR® Green qPCR ReadyMix™, For fast SYBR Green quantitative PCR

KCQS01

KiCqStart® SYBR® Green qPCR ReadyMix™, Low ROX™, for ABI and Stratagene instruments

KCQS02

KiCqStart® SYBR® Green qPCR ReadyMix™, with ROX™ for ABI instruments

KCQS03

KiCqStart® SYBR® Green qPCR ReadyMix™, iQ™, with fluorescein for Bio-Rad systems

Kod klasy składowania

12 - Non Combustible Liquids

Klasa zagrożenia wodnego (WGK)

WGK 3

Temperatura zapłonu (°F)

Not applicable

Temperatura zapłonu (°C)

Not applicable

Certyfikaty analizy (CoA)

Poszukaj Certyfikaty analizy (CoA), wpisując numer partii/serii produktów. Numery serii i partii można znaleźć na etykiecie produktu po słowach „seria” lub „partia”.

Masz już ten produkt?

Dokumenty związane z niedawno zakupionymi produktami zostały zamieszczone w Bibliotece dokumentów.

Odwiedź Bibliotekę dokumentów

Muthappa Senthil-Kumar et al.
Nature protocols, 9(7), 1549-1562 (2014-06-06)
Tobacco rattle virus (TRV)-based virus-induced gene silencing (VIGS) is widely used in various plant species to downregulate the expression of a target plant gene. TRV is a bipartite, positive-strand RNA virus with the TRV1 and TRV2 genomes. To induce post-transcriptional
Tsion Zewdu Minas et al.
Oncotarget, 8(21), 34141-34163 (2016-05-19)
Ewing sarcoma (ES) involves a tumor-specific chromosomal translocation that produces the EWS-FLI1 protein, which is required for the growth of ES cells both in vitro and in vivo. However, an EWS-FLI1-driven transgenic mouse model is not currently available. Here, we
YK-4-279 effectively antagonizes EWS-FLI1 induced leukemia in a transgenic mouse model.
Minas TZ
Oncotarget, 6(35), 37678-37694 (2015)
Phebe Verbrugghe et al.
BMC microbiology, 21(1), 23-23 (2021-01-13)
Since its discovery in 2007, the importance of the human gut bacterium Prevotella copri (P. copri) has been widely recognized with its links to diet and health status and potential as next generation probiotic. Therefore, precise, convenient and cost-effective diagnostic
NLRX1 Enhances Glutamate Uptake and Inhibits Glutamate Release by Astrocytes
Mahmoud S, et al.
Cells, 8(5), 400-400 (2019)
Wyświetl wszystkie powiązane dokumenty

Produkty

PCR/qPCR Data Analysis

After a traditional PCR has been completed, the PCR/qPCR data analysis is conducted by resolution through an agarose gel or, more recently, through a capillary.

Analiza danych PCR/qPCR

Po zakończeniu tradycyjnego PCR, analiza danych PCR/qPCR jest przeprowadzana przez rozdzielczość w żelu agarozowym lub, ostatnio, przez kapilarę.

PCR Assay Optimization and Validation

PCR assay guide navigates you through primer validation and other assay optimization factors to ensure high sensitivity and specificity for optimum DNA/ RNA quantification.

Optymalizacja i walidacja testów PCR

Przewodnik po teście PCR prowadzi przez walidację starterów i inne czynniki optymalizacji testu, aby zapewnić wysoką czułość i specyficzność dla optymalnej kwantyfikacji DNA / RNA.

Protokoły

KiCqStart™ Universal SYBR® Green qPCR Protocol

Quantitative PCR protocol using SYBR Green reagents. Procedure supports most qPCR instruments.

Primer Optimization Protocol Using Temperature Gradient

Gradient PCR for assay optimization is to determine the optimum annealing temperature (Ta) of the primers by testing identical reactions containing a fixed primer concentration, across a range of annealing temperatures.

qPCR Efficiency Determination Protocol

Once an assay has been optimized, it is important to verify the reaction efficiency. This information is important when reporting and comparing assays. In this example protocol, the assay efficiency is compared over a wide and narrow dynamic range of cDNA concentrations.

Protokół wyboru genów referencyjnych qPCR

Analiza danych ekspresji genów wymaga stabilnego odniesienia lub kontroli obciążenia. Odniesieniem jest zwykle jeden lub więcej genów referencyjnych.

Zobacz wszystko

Nasz zespół naukowców ma doświadczenie we wszystkich obszarach badań, w tym w naukach przyrodniczych, materiałoznawstwie, syntezie chemicznej, chromatografii, analityce i wielu innych dziedzinach.

Skontaktuj się z zespołem ds. pomocy technicznej