94149

Atto 647N-Streptavidin

BioReagent, suitable for fluorescence

• Synonim(y): Streptavidin-Atto 647N
• Kod UNSPSC: 12161900
• NACRES: NA.25

Właściwości

• linia produktu: BioReagent
• Poziom jakości: 100
• Postać: powder
• producent / nazwa handlowa: ATTO-TEC GmbH
• λ: in ethanol (with 0.1% trifluoroacetic acid)
• absorpcja UV: λ: 650.0-656.0 nm A_max
• przydatność: corresponds for gel electrophoresis; suitable for fluorescence
• temp. przechowywania: −20°C

Opis

Opis ogólny

Atto 647N is a new generation fluorescent dye dedicated towards the red spectral region. It is a cationic dye and carries a net electrical charge of +1 post substrate coupling reaction. Streptavidin, isolated from Streptomyces avidinii, is a tetrameric protein of 4 x 13.2 kDa which binds very tightly to the small molecule biotin. The dissociation constant of the complex is extremely small (Kd ≈ 10-15 M), ranking among the strongest non-covalent interactions. Atto streptavidin conjugates are readily soluble in water.

Zastosowanie

Atto 647N-Streptavidin is designed for application in the area of life science, e.g., labeling of DNA, RNA or proteins. Atto streptavidin conjugates may be used as secondary detection reagents in flow cytometry, immunoassays, blot analysis, and histochemical applications. Atto 647N -Streptavidin is suitable for multiplex analysis, including multicolor imaging.

Cechy i korzyści

Atto 647N- Streptavidin has the following benefits-

• Highly photostable. Strong absorption.
• Excellent fluorescent quantum yield.
• Highly resistant to ozone degradation.
• Minimal triplet formation.

Komentarz do analizy

dye-to-protein ratio ≥1.5

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Informacja o środkach bezpieczeństwa

• Kod klasy składowania: 11 - Combustible Solids
• Klasa zagrożenia wodnego (WGK): WGK 3
• Temperatura zapłonu (°F): Not applicable
• Temperatura zapłonu (°C): Not applicable
• Środki ochrony indywidualnej: Eyeshields, Gloves, type N95 (US)