93773

TWEEN^® 20

viscosity 250-450 mPa.s (25 °C)

• Synonim(y): Polyethylene glycol sorbitan monolaurate, Polyoxyethylenesorbitan monolaurate
• Numer CAS: 9005-64-5
• Numer MDL: MFCD00165986
• Kod UNSPSC: 12161900
• NACRES: NA.21

Właściwości

• opis: non-ionic
• Postać: viscous liquid
• masa cząsteczkowa: ~1228
• metody: DNA extraction: suitable; RNA extraction: suitable; cell culture | mammalian: suitable; protein purification: suitable
• współczynnik refrakcji: n20/D 1.468 (lit.); n20/D 1.471
• lepkość: 250-450 mPa.s(25 °C)
• wartość HLB: 16.7±1.0
• gęstość: 1.11 g/mL at 20 °C; 1.095 g/mL at 25 °C (lit.)
• ciąg SMILES: CCCCCCCCCCCC(=O)OCCOCC(C1C(C(CO1)OCCO)OCCO)OCCO
• InChI: 1S/C26H50O10/c1-2-3-4-5-6-7-8-9-10-11-24(30)34-19-18-31-20-22(32-15-12-27)26-25(35-17-14-29)23(21-36-26)33-16-13-28/h22-23,25-29H,2-21H2,1H3
• Klucz InChI: HMFKFHLTUCJZJO-UHFFFAOYSA-N

Opis

Opis ogólny

Tween^®20 is a polyoxyethylene sorbitol ester that belongs to the polysorbate family. It is a nonionic detergent having a molecular weight of 1,225 daltons, assuming 20 ethylene oxide units, 1 sorbitol, and 1 lauric acid as the primary fatty acid. The ethylene oxide subunits are responsible for the hydrophilic nature of the surfactant, while the hydrocarbon chains provide the hydrophobic environment. Sorbitol forms the backbone ring to which the ethylene oxide polymers are attached.

Zastosowanie

Tween^® 20 is widely used in several biochemical applications. It has been used:

• as an emulsifying agent for the preparation of stable oil-in-water emulsions
• in pre-extraction of membranes to remove peripheral proteins (used at 2% for extraction of membrane-bound proteins)
• as a blocking agent for membrane based immunoassays at a typical concentration of 0.05% - 1%
• for lysing mammalian cells at a concentration of 0.005 to 0.5%
• for rehydrating tissue before in situ hybridization

Non-ionic detergent

Działania biochem./fizjol.

Tween^® 20, functioning as a detergent, potentially operates by binding to the hydrophobic components of water-insoluble substances, thereby making them hydrophilic.

Cechy i korzyści

• Suitable for protein purification and DNA and RNA extraction
• Suitable for mammalian cell culture
• High purity product for research applications

Przestroga

may get turbid on storage, heat to clear

Inne uwagi

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Informacje prawne

TWEEN is a registered trademark of Croda International PLC

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Informacja o środkach bezpieczeństwa

• Kod klasy składowania: 10 - Combustible liquids
• Klasa zagrożenia wodnego (WGK): WGK 3
• Temperatura zapłonu (°F): 527.0 °F - Pensky-Martens closed cup
• Temperatura zapłonu (°C): 275 °C - Pensky-Martens closed cup
• Środki ochrony indywidualnej: Eyeshields, Gloves