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Merck

P7170

Sigma-Aldrich

Ponceau S solution

BioReagent, suitable (for use in cellulose acetate electrophoresis), 0.1 % (w/v) in 5% acetic acid

Synonim(y):

Ponceau S

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About This Item

Wzór empiryczny (zapis Hilla):
C22H12N4Na4O13S4
Numer CAS:
Masa cząsteczkowa:
760.57
Numer MDL:
Kod UNSPSC:
12171500
Identyfikator substancji w PubChem:

linia produktu

BioReagent

Poziom jakości

Postać

liquid

stężenie

0.1 % (w/v) in 5% acetic acid

gęstość

1.01 g/cm3 at 20 °C

przydatność

suitable (for use in cellulose acetate electrophoresis)
suitable for electrophoresis

Zastosowanie

diagnostic assay manufacturing
hematology
histology

temp. przechowywania

room temp

ciąg SMILES

[Na+].[Na+].[Na+].[Na+].Oc1c(\N=N\c2ccc(cc2S([O-])(=O)=O)\N=N\c3ccc(cc3)S([O-])(=O)=O)c4ccc(cc4cc1S([O-])(=O)=O)S([O-])(=O)=O

InChI

1S/C22H16N4O13S4.4Na/c27-22-20(43(37,38)39)10-12-9-16(41(31,32)33)6-7-17(12)21(22)26-25-18-8-3-14(11-19(18)42(34,35)36)24-23-13-1-4-15(5-2-13)40(28,29)30;;;;/h1-11,27H,(H,28,29,30)(H,31,32,33)(H,34,35,36)(H,37,38,39);;;;/q;4*+1/p-4/b24-23+,26-25+;;;;

Klucz InChI

KQHKSGRIBYJYFX-WNDMICSESA-J

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Opis ogólny

Ponceau S is the sodium salt of a diazo dye. Ponceau S solution is a suitable reagent for use in electrophoresis studies. The Ponceau S stain comes ready-to-use and is designed for rapid (5 min) staining of protein bands on nitrocellulose or PVDF membranes (Western blots) and also for staining protein on cellulose acetate membranes. It may be employed as stain for the purified fibrinolytic enzyme (CK) for the analysis of N-terminal amino acid sequence of the first 14 residues of CK by a protein sequencer. Ponceau S staining on nitrocellulose and PVDF is reversible by washing the stained membrane with 0.1M NaOH for 1 minute. The stained and destained membrane can then be immunologically detected (Western blotting). A product information sheet is supplied which includes the staining procedure for cellulose acetate, nitrocellulose, and PVDF.

Zastosowanie

Ponceau S solution has been used for membrane staining (Western blotting).
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Kod klasy składowania

10 - Combustible liquids

Klasa zagrożenia wodnego (WGK)

WGK 1

Temperatura zapłonu (°F)

Not applicable

Temperatura zapłonu (°C)

Not applicable


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Dokumenty związane z niedawno zakupionymi produktami zostały zamieszczone w Bibliotece dokumentów.

Odwiedź Bibliotekę dokumentów

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Endocrine Journal (2009)
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The protozoan parasite Trypanosoma cruzi, the causative agent of Chagas disease, causes severe morbidity and mortality in afflicted individuals. About 30% of T. cruzi-infected individuals present with cardiac, gastrointestinal tract, and/or neurological disorders. Megacolon, one of the major pathologies of
Legume Grains Enhance Ileal Losses of Specific Endogenous Serine-Protease Proteins in Weaned Pigs1
Paulo Salgado
The Journal of Nutrition (2002)
Challa S.S.R. Kumar
Semiconductor Nanomaterials (2010)

Produkty

To meet the great diversity of protein analysis needs, Sigma offers a wide selection of protein visualization (staining) reagents. EZBlue™ and ProteoSilver™, designed specifically for proteomics, also perform impressively in traditional PAGE formats.

Protokoły

Protokół Western blot szczegółowo opisuje przenoszenie białek z żeli na nitrocelulozę, co ma kluczowe znaczenie dla procedur immunoblottingu w badaniach naukowych.

Nasz zespół naukowców ma doświadczenie we wszystkich obszarach badań, w tym w naukach przyrodniczych, materiałoznawstwie, syntezie chemicznej, chromatografii, analityce i wielu innych dziedzinach.

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