P7170

Ponceau S solution

BioReagent, 0.1 % (w/v) in 5% acetic acid

• Synonim(y): Ponceau S
• Wzór empiryczny (zapis Hilla): C₂₂H₁₂N₄Na₄O₁₃S₄
• Numer CAS: 6226-79-5
• Masa cząsteczkowa: 760.57
• Numer MDL: MFCD00003892
• Kod UNSPSC: 12171500
• Identyfikator substancji w PubChem: 24898827
• NACRES: NA.47

Właściwości

• product name: Ponceau S solution, BioReagent, suitable (for use in cellulose acetate electrophoresis), 0.1 % (w/v) in 5% acetic acid
• klasa czystości: for protein analysis
• Poziom jakości: 200
• linia produktu: BioReagent
• Postać: liquid
• stężenie: 0.1 % (w/v) in 5% acetic acid
• metody: western blot: suitable
• gęstość: 1.01 g/cm³ at 20 °C
• przydatność: suitable (for use in cellulose acetate electrophoresis); suitable for electrophoresis
• Zastosowanie: diagnostic assay manufacturing; hematology; histology
• temp. przechowywania: room temp
• ciąg SMILES: [Na+].[Na+].[Na+].[Na+].Oc1c(\N=N\c2ccc(cc2S([O-])(=O)=O)\N=N\c3ccc(cc3)S([O-])(=O)=O)c4ccc(cc4cc1S([O-])(=O)=O)S([O-])(=O)=O
• InChI: 1S/C22H16N4O13S4.4Na/c27-22-20(43(37,38)39)10-12-9-16(41(31,32)33)6-7-17(12)21(22)26-25-18-8-3-14(11-19(18)42(34,35)36)24-23-13-1-4-15(5-2-13)40(28,29)30;;;;/h1-11,27H,(H,28,29,30)(H,31,32,33)(H,34,35,36)(H,37,38,39);;;;/q;4*+1/p-4/b24-23+,26-25+;;;;
• Klucz InChI: KQHKSGRIBYJYFX-WNDMICSESA-J

Opis

Opis ogólny

Ponceau S is the sodium salt of a diazo dye. Ponceau S solution is a suitable reagent for use in electrophoresis studies. The Ponceau S stain comes ready-to-use and is designed for rapid (5 min) staining of protein bands on nitrocellulose or PVDF membranes (Western blots) and also for staining protein on cellulose acetate membranes. It may be employed as stain for the purified fibrinolytic enzyme (CK) for the analysis of N-terminal amino acid sequence of the first 14 residues of CK by a protein sequencer. Ponceau S staining on nitrocellulose and PVDF is reversible by washing the stained membrane with 0.1M NaOH for 1 minute. The stained and destained membrane can then be immunologically detected (Western blotting). A product information sheet is supplied which includes the staining procedure for cellulose acetate, nitrocellulose, and PVDF.

Zastosowanie

Ponceau S solution has been used for membrane staining (Western blotting).

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Informacja o środkach bezpieczeństwa

• Kod klasy składowania: 10 - Combustible liquids
• Klasa zagrożenia wodnego (WGK): WGK 1
• Temperatura zapłonu (°F): Not applicable
• Temperatura zapłonu (°C): Not applicable