C1359

Calcein AM solution

≥90% purity (HPLC), solution

• Wzór empiryczny (zapis Hilla): C₄₆H₄₆N₂O₂₃
• Numer CAS: 148504-34-1
• Masa cząsteczkowa: 994.86
• Numer WE: 200-664-3
• Numer MDL: MFCD05861516
• Kod UNSPSC: 12171500
• Identyfikator substancji w PubChem: 24892405
• NACRES: NA.47

Właściwości

• product name: Calcein AM solution, 4 mM in DMSO, ≥90% (HPLC), solution
• Poziom jakości: 100
• Próba: ≥90% (HPLC)
• Postać: solution
• stężenie: 4 mM in DMSO
• kolor: colorless; white to off-white
• λmaks.: 491-497 nm
• Zastosowanie: diagnostic assay manufacturing; hematology; histology
• temp. przechowywania: −20°C
• ciąg SMILES: CC(=O)OCOC(=O)CN(CC(=O)OCOC(C)=O)Cc1c(OC(C)=O)ccc2c1Oc3c(CN(CC(=O)OCOC(C)=O)CC(=O)OCOC(C)=O)c(OC(C)=O)ccc3C24OC(=O)c5ccccc45
• InChI: 1S/C46H46N2O23/c1-25(49)60-21-64-39(55)17-47(18-40(56)65-22-61-26(2)50)15-32-37(68-29(5)53)13-11-35-43(32)70-44-33(16-48(19-41(57)66-23-62-27(3)51)20-42(58)67-24-63-28(4)52)38(69-30(6)54)14-12-36(44)46(35)34-10-8-7-9-31(34)45(59)71-46/h7-14H,15-24H2,1-6H3
• Klucz InChI: XKFSBWQWNMZWFA-UHFFFAOYSA-N

Opis

Zastosowanie

In cells, calcein AM solution has been used in calcein-AM dye transfer assay. It has been used for the staining of cells to study quantification of cell-migration. It has been used to study cytotoxic effects of drugs in cells.

Działania biochem./fizjol.

Calcein AM is a membrane permeable nonfluorescent molecule. It is converted to fluorescent calcein by intracellular esterases. Calcein has limited membrane permeability. Calcein AM is also a substrate for p-glycoprotein (Pgp). Presence of Pgp results in efflux of calcein AM and thereby decreases cellular fluorescence. On the other hand, presence of Pgp inhibitor increases cellular entry of calcein AM and thereby increases cellular fluorescence. Calcein AM plays an important role in early drug discovery.

Fluorescent cell permeable derivative of calcein.

Postać fizyczna

Solution in 0.4% Calcein AM and 99.6% DMSO.

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Informacja o środkach bezpieczeństwa

• Kod klasy składowania: 10 - Combustible liquids
• Klasa zagrożenia wodnego (WGK): WGK 2
• Temperatura zapłonu (°F): 185.0 °F - closed cup
• Temperatura zapłonu (°C): 85 °C - closed cup
• Środki ochrony indywidualnej: Eyeshields, Gloves, multi-purpose combination respirator cartridge (US)