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Lysozyme from chicken egg white
powder (crystalline), 70000-140000 U/mg
Synonim(y):
Mucopeptide N-acetylmuramoylhydrolase, Muramidase
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About This Item
Numer CAS:
Numer WE:
Numer MDL:
Kod UNSPSC:
12352204
NACRES:
NA.22
Polecane produkty
pochodzenie biologiczne
chicken egg white
Poziom jakości
Formularz
powder (crystalline)
aktywność właściwa
70000-140000 U/mg
masa cząsteczkowa
single-chain 14.3 kDa
Mr ~14600
metody
cell based assay: suitable
przydatność
suitable for cell lysis
numer dostępu UniProt
Zastosowanie
cell analysis
temp. przechowywania
2-8°C
informacje o genach
chicken ... LYZ(396218)
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Zastosowanie
Enzyme breaks down the cell walls of bacteria; used to prepare spheroplasts.
Działania biochem./fizjol.
Lysozyme hydrolyzes β(1→4) linkages between N-acetylmuramic acid and N-acetyl-D-glucosamine residues in peptidoglycan and between N-acetyl-D-glucosamine residues in chitodextrin. Gram-positive cells are quite susceptible to this hydrolysis as their cell walls have a high proportion of peptidoglycan. Gram-negative bacteria are less susceptible due to the presence of an outer membrane and a lower proportion of peptidoglycan. However, these cells may be hydrolyzed in the presence of EDTA that chelates metal ions in the outer bacterial membrane.
The enzyme is active over a broad pH range (6.0 to 9.0). At pH 6.2, maximal activity is observed over a wider range of ionic strengths (0.02 to 0.100 M) than at pH 9.2 (0.01 to 0.06 M).
The enzyme is active over a broad pH range (6.0 to 9.0). At pH 6.2, maximal activity is observed over a wider range of ionic strengths (0.02 to 0.100 M) than at pH 9.2 (0.01 to 0.06 M).
Lysozyme hydrolyzes β(1→4) linkages between N-acetylmuramic acid and N-acetyl-D-glucosamine residues in peptidoglycan and between N-acetyl-D-glucosamine residues in chitodextrin. Gram-positive cells are quite susceptible to this hydrolysis as their cell walls have a high proportion of peptidoglycan.
Definicja jednostki
1 U corresponds to the amount of enzyme which decreases the absorbance at 450 nm by 0.001 per minute at pH 7.0 and 25°C (Micrococcus luteus, ATCC 4698, as substrate)
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Hasło ostrzegawcze
Danger
Zwroty wskazujące rodzaj zagrożenia
Zwroty wskazujące środki ostrożności
Klasyfikacja zagrożeń
Resp. Sens. 1
Kod klasy składowania
11 - Combustible Solids
Klasa zagrożenia wodnego (WGK)
WGK 3
Temperatura zapłonu (°F)
Not applicable
Temperatura zapłonu (°C)
Not applicable
Środki ochrony indywidualnej
Eyeshields, Gloves, type N95 (US)
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