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48722

Millipore

Gelatin from porcine skin

medium gel strength, suitable for microbiology

Synonim(y):

Gelatine, hydrolyzed collagen, collagen hydrolysate, gelatine hydrolysate

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About This Item

Numer CAS:
Numer WE:
Numer MDL:
Kod UNSPSC:
41106212
NACRES:
NA.85

pochodzenie biologiczne

Porcine

Poziom jakości

sterylność

non-sterile

Postać

powder

jakość

medium gel strength

opakowanie

bottle of 100 g
bottle of 500 g

pozostałość po prażeniu

≤2%

strata

≤15% loss on drying

kolor

light yellow

pH

4.0-6.0 (25 °C, 67 mg/mL in H2O)

siła żelu

165-195 g Bloom (67 mg/ml water)

rozpuszczalność

H2O: 67 mg/mL at 50 °C, slightly hazy, slightly yellow

ślady anionów

chloride (Cl-): ≤3000 mg/kg

ślady kationów

Ca: ≤2000 mg/kg
Cd: ≤5 mg/kg
Co: ≤5 mg/kg
Cr: ≤10 mg/kg
Cu: ≤50 mg/kg
Fe: ≤50 mg/kg
K: ≤500 mg/kg
Mg: ≤500 mg/kg
Mn: ≤5 mg/kg
Na: ≤5000 mg/kg
Ni: ≤5 mg/kg
Pb: ≤5 mg/kg
Zn: ≤10 mg/kg

Zastosowanie

microbiology

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Opis ogólny

Gelatin from porcine skin is generated from the acidic digestion of collagen and is referred to as type A. It is a hydrocolloid and is rich in glycine, proline, and hydroxyproline, which impart structural stability. It is synthesized from the alkaline digestion of collagen from porcine. Gelatin takes up a random coil structure after digestion from the triple helical collagen.

Gelatin is a heterogeneous mixture of water-soluble proteins of high average molecular masses present in collagen. Proteins are extracted by boiling the relevant skin, tendons, ligaments, bones, etc., in water. Type A gelatin is derived from acid-cured tissue, and Type B from lime-cured tissue.

The Bloom number, determined by the Bloom gelometer, is an indication of the strength of a gel formed from a solution of the known concentration. The Bloom number is proportional to the average molecular mass. Bloom numbers of porcine skin Gelatin vary from 90 to 300 g. This product has a gel strength of 180.

Zastosowanie

Gelatin has been used in many applications, such as in coating cell culture plates to improve attachment of cells, in PCR to stabilize Taq DNA, as a blocking reagent in Western blotting, ELISA, and immunochemistry, and as a component of media for species differentiation in bacteriology.

Komponenty

Gelatin is a heterogeneous mixture of water-soluble proteins of high average molecular masses, present in collagen. Proteins are extracted by boiling the relevant skin, tendons, ligaments, bones, etc. in water. Type A gelatin is derived from acid-cured tissue. Type B is derived from lime-cured tissue.

Przestroga

Dry gelatin, when stored in airtight containers at room temperature, will remain unchanged for many years. When heated at 100°C in the presence of air, it swells becomes soft and disintegrates to a carbonaceous mass with evolution of pyridine bases and ammonia.

Uwaga dotycząca przygotowania

This product is derived from porcine skin. Gelatin is soluble in hot than in cold water. It is practically insoluble in most organic solvents such as alcohol, chloroform, carbon disulfide, carbon tetrachloride, ether, benzene, acetone, and oils. Manufactured by Gelita AG
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Kod klasy składowania

11 - Combustible Solids

Klasa zagrożenia wodnego (WGK)

nwg

Środki ochrony indywidualnej

Eyeshields, Gloves, type N95 (US)


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