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11011375001
Roche
Nutridoma™-SP
suitable for hybridoma, solution, pkg of 100 mL (100x)
Synonim(y):
hybridoma, nutridoma
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About This Item
Kod UNSPSC:
12352207
Polecane produkty
sterylność
sterile; 0.2 μm filtered
Poziom jakości
Postać
solution
opakowanie
pkg of 100 mL (100x)
producent / nazwa handlowa
Roche
stężenie
100 ×
metody
cell culture | hybridoma: suitable
zanieczyszczenia
mycoplasma, tested
≤100 EU/mL Endotoxin (LAL test)
rozpuszczalność
water: miscible
temp. przechowywania
20-25°C
Powiązane kategorie
Opis ogólny
Nutridoma is a family of chemically defined supplements that can completely replace serum in cell culture media. Nutridoma-SP is used for the cultivation of established myeloma and hybridoma cells. It is used for the serum-free cultivation of murine myelomas and hybridomas that have an intact cholesterol biosynthesis pathway, such as those derived from the SP2/0, and some of the P3x63Ag8.653 cell lines and lymphoblastoid cell lines, as well as for primary lymphoid cell cultures. It is also used for the serum-free culture of a variety of other cell types, including neural explants.
Zastosowanie
Nutridoma-SP has been used in the culture media for canine trophoblasts, human embryonic stem cells and PLB-985 cells.
Definicja jednostki
Endotoxin (LAL): 10 EU/ml; mycoplasma-tested
Postać fizyczna
Solution, 100x concentrated, filtered through 0.2 μm pore size membrane
Biochemically defined serum-free medium supplement composed of albumin, insulin, transferrin, and other defined organic and inorganic compounds. Nutridoma contains no other growth factors, mitogens, hormones, or sterols.
The solution contains the pH indicator phenol red and appears clear.
Biochemically defined serum-free medium supplement composed of albumin, insulin, transferrin, and other defined organic and inorganic compounds. Nutridoma contains no other growth factors, mitogens, hormones, or sterols.
The solution contains the pH indicator phenol red and appears clear.
Uwaga dotycząca przygotowania
Working concentration: Nutridoma-SP (100x) is diluted 1:100 (v/v) with sterile basal medium. Use high glucose DMEM/RPMI 1640 (1:1) (R/D medium) for Nutridoma-SP. The final medium should also contain L-glutamine and sodium bicarbonate. The protein concentration is less than 50 μg/ml for 1% working concentration.
Storage conditions (working solution): 2 to 8 °C
Nutridoma working solutions, e.g., media containing 1% Nutridoma (1%) are stable for 4 weeks when stored at 2 to 8 °C in the dark. Do not freeze.
Note: Do not store in plastic as adsorption of ingredients to the plastic surface may occur.
Storage conditions (working solution): 2 to 8 °C
Nutridoma working solutions, e.g., media containing 1% Nutridoma (1%) are stable for 4 weeks when stored at 2 to 8 °C in the dark. Do not freeze.
Note: Do not store in plastic as adsorption of ingredients to the plastic surface may occur.
Inne uwagi
For life science research only. Not for use in diagnostic procedures.
Informacje prawne
Nutridoma is a trademark of Roche
This page may contain text that has been machine translated.
Kod klasy składowania
12 - Non Combustible Liquids
Klasa zagrożenia wodnego (WGK)
nwg
Temperatura zapłonu (°F)
No data available
Temperatura zapłonu (°C)
No data available
Certyfikaty analizy (CoA)
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