10745782001
Roche
DNA, lambda
from bacteriophage lambda cl857 Sam 7
Synonim(y):
lambda dna
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About This Item
Kod UNSPSC:
41106310
Polecane produkty
Poziom jakości
Postać
solution
masa cząsteczkowa
32,300 kDa
opakowanie
pkg of 1 mL
producent / nazwa handlowa
Roche
warunki przechowywania
avoid repeated freeze/thaw cycles
stężenie
250 μg/mL
zanieczyszczenia
RNA, none detected
nuclease, none detected
kolor
colorless
rozpuszczalność
water: miscible
Warunki transportu
dry ice
temp. przechowywania
−20°C (−15°C to −25°C)
Powiązane kategorie
Opis ogólny
The DNA of bacteriophage lambda contains 48,502 nucleotide pairs forming a linear duplex. The 5′ ends of this DNA molecule have self-complementary single-stranded protrusions of 12 nucleotides that can anneal to form circles. Upon entry into the host cell, the DNA circularizes in the presence of DNA ligase. Many such circles are generated during the early lytic infection. At a later stage in replication, these individual circles/rings are converted to linear concatemers by a process called rolling-circle replication. The linear concatemers are cleaved during packaging to form monomeric chromosomal units by the enzyme DNA terminase.
Zastosowanie
DNA, lambda has been used to spike peripheral blood leukocytes (PBL) before genomic DNA isolation. It has also been used in the preparation of biotinylated double-stranded DNA (dsDNA) substrate.
Jakość
The DNA shows the typical cleavage pattern for wild type λDNA in gel electrophoresis after cleavage with restriction endonucleases EcoR I and Hind III.
Sekwencja
Chain Length 48,502 bp
Postać fizyczna
Solution, 250 μg/ml, in 10 mM Tris-HCl, 1 mM EDTA, pH 8.0, ready-to-use
Przechowywanie i stabilność
Store at -15 to -25°C until use; once thawed, store at 2-8 °C.
Inne uwagi
For life science research only. Not for use in diagnostic procedures.
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Kod klasy składowania
12 - Non Combustible Liquids
Klasa zagrożenia wodnego (WGK)
nwg
Temperatura zapłonu (°F)
No data available
Temperatura zapłonu (°C)
No data available
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