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Merck

10106399001

Roche

GTP

>90% (HPLC), crystals, pkg of 250 mg

Synonim(y):

Guanosine 5′-triphosphate sodium salt hydrate, 5′-GTP-Na2

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About This Item

Wzór empiryczny (zapis Hilla):
C10H16N5O14P3 · xNa+ · yH2O
Numer CAS:
Masa cząsteczkowa:
523.18 (anhydrous free acid basis)
Beilstein:
4113439
Numer MDL:
Kod UNSPSC:
41106305
Identyfikator substancji w PubChem:

opis

Disodium salt

Poziom jakości

Próba

>90% (HPLC)

Postać

crystals

masa cząsteczkowa

Mr 523.2 (GTP)
Mr 567.1 (GTP-Na2)

opakowanie

pkg of 250 mg

producent / nazwa handlowa

Roche

warunki przechowywania

avoid repeated freeze/thaw cycles

ciąg SMILES

O.O.[Na+].[Na+].NC1=Nc2c(ncn2[C@@H]3O[C@H](COP(O)(=O)OP([O-])(=O)OP(O)([O-])=O)[C@@H](O)[C@H]3O)C(=O)N1

InChI

1S/C10H16N5O14P3.2Na.2H2O/c11-10-13-7-4(8(18)14-10)12-2-15(7)9-6(17)5(16)3(27-9)1-26-31(22,23)29-32(24,25)28-30(19,20)21;;;;/h2-3,5-6,9,16-17H,1H2,(H,22,23)(H,24,25)(H2,19,20,21)(H3,11,13,14,18);;;2*1H2/q;2*+1;;/p-2/t3-,5-,6-,9-;;;;/m1..../s1

Klucz InChI

HPLNHYUZFVUXFJ-ZVQJTLEUSA-L

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Opis ogólny

GTP functions as a carrier of phosphates and pyrophosphates involved in channeling chemical energy into specific biosynthetic pathways. It also serves as an energy-rich precursor of mononucleotide units in the enzymatic biosynthesis of DNA and RNA. GTP is involved in the initiation of peptide synthesis during the binding of formylmethionyl-tRNA (F-met-tRNA) to the ribosome. It is also involved in polypeptide chain elongation. GTP is hydrolyzed by tubulin and its hydrolysis is accompanied by microtubule assembly.
GTP is supplied as the disodium salt in crystal form.

Zastosowanie

GTP, disodium salt, is required as a coenzyme for protein biosynthesis in a cell-free system. It has been used in FtsZ polymerization assay and in the preparation of pipette solution for the electrophysiological recordings of the whole-cell membrane currents.

Jakość

Purity: 76% GTP (enzymatic), >90 area% (HPLC), 9% Na+, 8% H2O

Uwaga dotycząca przygotowania

Activator: EDTA prevents the formation of enzyme aggregates. Phosphate stimulates the NADH-dependent reaction.
Storage conditions (working solution): Aqueous solutions (pH 7.0) are stable at 2 to 8 °C or frozen for approx. one week, for reasons of hydrolysis pH should not be < 3.

If neutralized with water preparation can be stored frozen at -15 to -25 °C in small portions, one sample should thawed only once and should be used within the next one or two days (stored at 2 to 8 °C). Preparation can also be dissolved in a buffer pH 7-8 without neutralization (buffer concentration should be high enough that pH is not changed by addition of those substances); solution can be stored frozen.

Investigations on the storage temperature 2 to 8 °C compared to -15 to -25 °C showed no difference.

Przechowywanie i stabilność

Store at 2 to 8 °C. (Store dry!)

Inne uwagi

For life science research only. Not for use in diagnostic procedures.
This page may contain text that has been machine translated.

Kod klasy składowania

11 - Combustible Solids

Klasa zagrożenia wodnego (WGK)

WGK 1

Temperatura zapłonu (°F)

Not applicable

Temperatura zapłonu (°C)

Not applicable


Certyfikaty analizy (CoA)

Poszukaj Certyfikaty analizy (CoA), wpisując numer partii/serii produktów. Numery serii i partii można znaleźć na etykiecie produktu po słowach „seria” lub „partia”.

Masz już ten produkt?

Dokumenty związane z niedawno zakupionymi produktami zostały zamieszczone w Bibliotece dokumentów.

Odwiedź Bibliotekę dokumentów

Lehninger, A.L
Biochemistry, 316-316 (1977)
Marzia Govoni
Bio-protocol, 7(3), e2120-e2120 (2017-02-05)
In eukaryotic cells transcriptional processes are carried out by three different RNA polymerases: RNA polymerase I which specifically transcribes ribosomal RNA (rRNA), RNA polymerase II which transcribes protein-coding genes to yield messenger RNAs (mRNAs) and small RNAs, while RNA polymerase
Ewa Król et al.
Journal of visualized experiments : JoVE, (81)(81), e50844-e50844 (2013-12-05)
During bacterial cell division, the essential protein FtsZ assembles in the middle of the cell to form the so-called Z-ring. FtsZ polymerizes into long filaments in the presence of GTP in vitro, and polymerization is regulated by several accessory proteins.
The role of guanosine 5'-triphosphate in the initiation of peptide synthesis. 3. Binding of formylmethionyl-tRNA to ribosomes.
T Ohta et al.
Proceedings of the National Academy of Sciences of the United States of America, 58(4), 1638-1644 (1967-10-01)
The role of guanosine 5'-triphosphate in polypeptide chain elongation.
Y Kaziro
Biochimica et biophysica acta, 505(1), 95-127 (1978-09-21)

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