SCC042
TC28a2 Human Chondrocyte Cell Line
Human
Synonim(y):
T/C-28a2
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About This Item
Kod UNSPSC:
41106514
eCl@ss:
32011203
NACRES:
NA.81
pochodzenie biologiczne:
human
Polecane produkty
Nazwa produktu
TC28a2 Human Chondrocyte Cell Line, TC28a2 Human Chondrocyte Cell Line is widely used as a model cell line for studying normal and pathological cartilage repair mechanisms related to chondrocyte biology and physiology.
pochodzenie biologiczne
human
Poziom jakości
metody
cell culture | mammalian: suitable
Warunki transportu
ambient
Opis ogólny
The chondrocyte is the only specialized cell type present in articular cartilage. They produce secretions to support and repair the cartilage matrix. Human chondrocytes in mature articular cartilage are post-mitotic and terminally differentiated cells. Studies on human chondrocytes have been hampered by the difficulty in obtaining sufficient numbers of primary human chondrocytes from a single joint and by variabilities among donors due to factors such as age and medical conditions.
T/C-28a2 cell line was established by transfecting primary cultures (day 5) of costal cartilage from a 15-year-old female with a retroviral vector expressing simian virus SV40 large T antigen.
References:
1. Goldring, M.B, Birkhead, J.R., Suen, L.F., Yamin, R., Mizuno, S., Glowacki, J., Arbiser, J.L, Apperley, J.F. (1994) Interleukin-1 beta-modulated gene expression in immortalized human chondrocytes. J. Clin. Invest 94(6): 2307-2316.
2. Claassen, H., Schicht, M., Brandt, J., Reuse, K., Schadlich, R., Goldring, M.B., Guddat, S.S., Thate, A., Paulsen, F. C-28/I2 and T/C-28a2 chondrocytes as well as human primary articular chondrocytes express hormone and insulin receptors- Useful cells in study of cartilage metabolism Ann Anat. 193(1): 23-29.
3. Goldring, M.B. (2004) Culture of immortalized chondrocytes and their use as models of chondrocyte function. Methods Mol. Med. 100: 37-52.
T/C-28a2 cell line was established by transfecting primary cultures (day 5) of costal cartilage from a 15-year-old female with a retroviral vector expressing simian virus SV40 large T antigen.
References:
1. Goldring, M.B, Birkhead, J.R., Suen, L.F., Yamin, R., Mizuno, S., Glowacki, J., Arbiser, J.L, Apperley, J.F. (1994) Interleukin-1 beta-modulated gene expression in immortalized human chondrocytes. J. Clin. Invest 94(6): 2307-2316.
2. Claassen, H., Schicht, M., Brandt, J., Reuse, K., Schadlich, R., Goldring, M.B., Guddat, S.S., Thate, A., Paulsen, F. C-28/I2 and T/C-28a2 chondrocytes as well as human primary articular chondrocytes express hormone and insulin receptors- Useful cells in study of cartilage metabolism Ann Anat. 193(1): 23-29.
3. Goldring, M.B. (2004) Culture of immortalized chondrocytes and their use as models of chondrocyte function. Methods Mol. Med. 100: 37-52.
Opis linii komórkowej
Chondrocyte Cells
Zastosowanie
This product is intended for sale and sold solely to academic institutions for internal academic research use per the terms of the “Academic Use Agreement” as detailed in the product documentation. For information regarding any other use, please contact licensing@emdmillipore.com.
Jakość
• Each vial contains ≥ 1X106 viable cells.
• Cells are tested by PCR and are negative for HPV-16, HPV-18, Hepatitis A, B, C and HIV-1 & 2 viruses.
• Cells are negative for mycoplasma contamination.
• Each lot of cells are genotyped by STR analysis to verify the unique identity of the cell line.
• Cells are tested by PCR and are negative for HPV-16, HPV-18, Hepatitis A, B, C and HIV-1 & 2 viruses.
• Cells are negative for mycoplasma contamination.
• Each lot of cells are genotyped by STR analysis to verify the unique identity of the cell line.
Przechowywanie i stabilność
Store in liquid nitrogen. The cells can be cultured for at least 10 passages after initial thawing without significantly affecting the cell marker expression and functionality.
Inne uwagi
Concentration: Please refer to lot specific datasheet.
Oświadczenie o zrzeczeniu się odpowiedzialności
RESEARCH USE ONLY. This product is regulated in France when intended to be used for scientific purposes, including for import and export activities (Article L 1211-1 paragraph 2 of the Public Health Code). The purchaser (i.e. enduser) is required to obtain an import authorization from the France Ministry of Research referred in the Article L1245-5-1 II. of Public Health Code. By ordering this product, you are confirming that you have obtained the proper import authorization.
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Kod klasy składowania
10 - Combustible liquids
Klasa zagrożenia wodnego (WGK)
WGK 3
Temperatura zapłonu (°F)
Not applicable
Temperatura zapłonu (°C)
Not applicable
Certyfikaty analizy (CoA)
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M B Goldring et al.
The Journal of clinical investigation, 94(6), 2307-2316 (1994-12-01)
Immortalized human chondrocytes were established by transfection of primary cultures of juvenile costal chondrocytes with vectors encoding simian virus 40 large T antigen and selection in suspension culture over agarose. Stable cell lines were generated that exhibited chondrocyte morphology, continuous
Mary B Goldring
Methods in molecular medicine, 100, 37-52 (2004-07-29)
Immortalization of chondrocytes increases life span and proliferative capacity but does not necessarily stabilize the differentiated phenotype. Expansion of chondrocyte cell lines in continuous monolayer culture may result in the loss of phenotype, particularly if high cell density is not
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