OP143
Anti-MDM2 (Ab-3) Mouse mAb (4B11)
liquid, clone 4B11, Calbiochem®
Synonim(y):
Anti-Ubiquitin Protein Ligase, Anti-p53 Binding Protein, Anti-Murine Double Minute Chromosome-2
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About This Item
Kod UNSPSC:
12352203
NACRES:
NA.41
Polecane produkty
pochodzenie biologiczne
mouse
Poziom jakości
forma przeciwciała
purified antibody
rodzaj przeciwciała
primary antibodies
klon
4B11, monoclonal
Postać
liquid
nie zawiera
preservative
reaktywność gatunkowa
mouse, human
producent / nazwa handlowa
Calbiochem®
warunki przechowywania
OK to freeze
avoid repeated freeze/thaw cycles
izotyp
IgG2a
Warunki transportu
wet ice
temp. przechowywania
−20°C
docelowa modyfikacja potranslacyjna
unmodified
informacje o genach
human ... MDM2(4193)
mouse ... Mdm2(17246)
Opis ogólny
Purified mouse monoclonal antibody. Recognizes the ~90 kDa (apparent MW) MDM2 protein.
Recognizes the ~90 kDa (apparent MW) MDM2 protein in A549 cells. May also recognize the ~60 kDa and ~90 kDa isoforms of MDM2.
This Anti-MDM2 (Ab-3) Mouse mAb (4B11) is validated for use in Immunoblotting, Immunofluorescence, Immunoprecipitation, Paraffin Sections for the detection of MDM2 (Ab-3).
Immunogen
Epitope: within amino acids 383-491
Human
full-length, recombinant human MDM2
Zastosowanie
Immunoblotting (2 g/ml, chemiluminescence)
Immunofluorescence (1 g/ml)
Immunoprecipitation (1 g/reaction)
Paraffin Sections (2.5 g/ml, heat pre-treatment required)
Opakowanie
Please refer to vial label for lot-specific concentration.
Ostrzeżenie
Toxicity: Standard Handling (A)
Postać fizyczna
In 50 mM sodium phosphate buffer, 50% glycerol.
Rekonstytucja
Following initial thaw, aliquot and freeze (-20°C).
Komentarz do analizy
Positive Control
A549 cells
A549 cells
Inne uwagi
Marchetti, A., et al. 1995. J. Pathol.175, 31.
Barak, Y., et al. 1993. EMBO. J.12, 461.
Ladanyi, M., et al. 1993. Cancer Res.53, 16.
Leach, F. S., et al. 1993. Cancer Res.53, 2231.
Oliner, J. D., et al. 1993. Nature362, 857.
Momand, J., et al. 1992. Cell69, 1237.
Oliner, J. D., et al. 1992. Nature358, 80.
Fakharzadeh, S. S., et al. 1991. EMBO. J.10, 1565.
Barak, Y., et al. 1993. EMBO. J.12, 461.
Ladanyi, M., et al. 1993. Cancer Res.53, 16.
Leach, F. S., et al. 1993. Cancer Res.53, 2231.
Oliner, J. D., et al. 1993. Nature362, 857.
Momand, J., et al. 1992. Cell69, 1237.
Oliner, J. D., et al. 1992. Nature358, 80.
Fakharzadeh, S. S., et al. 1991. EMBO. J.10, 1565.
May also recognize the ~60 kDa and ~90 kDa isoforms of MDM2. Antibody should be titrated for optimal results in individual systems.
Informacje prawne
CALBIOCHEM is a registered trademark of Merck KGaA, Darmstadt, Germany
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Kod klasy składowania
10 - Combustible liquids
Klasa zagrożenia wodnego (WGK)
WGK 3
Certyfikaty analizy (CoA)
Poszukaj Certyfikaty analizy (CoA), wpisując numer partii/serii produktów. Numery serii i partii można znaleźć na etykiecie produktu po słowach „seria” lub „partia”.
Masz już ten produkt?
Dokumenty związane z niedawno zakupionymi produktami zostały zamieszczone w Bibliotece dokumentów.
F S Leach et al.
Cancer research, 53(10 Suppl), 2231-2234 (1993-05-15)
The p53 and MDM2 genes were analyzed in 24 human soft tissue sarcomas (11 malignant fibrous histiocytomas and 13 liposarcomas). Alterations of p53, consisting of point mutations, deletions, or overexpression, were detected in one-third (8 of 24) of the sarcomas.
M Ladanyi et al.
Cancer research, 53(1), 16-18 (1993-01-01)
The human homologue of the murine double minute 2 gene (MDM2), a p53-binding protein which may act as a regulator of p53 protein function, has recently been cloned. Initial studies of this gene in a variety of human tumors have
J Momand et al.
Cell, 69(7), 1237-1245 (1992-06-26)
A cellular phosphoprotein with an apparent molecular mass of 90 kd (p90) that forms a complex with both mutant and wild-type p53 protein has been characterized, purified, and identified. The protein was identified as a product of the murine double
A Marchetti et al.
The Journal of pathology, 175(1), 31-38 (1995-01-01)
This study investigates the mdm2 gene status and expression in 66 surgically resected human breast carcinomas, with correlations with clinico-pathological and biological data (histological type, grading, steroid receptor status, p53 expression, proliferative activity). Four (7.7 per cent) out of 52
J D Oliner et al.
Nature, 362(6423), 857-860 (1993-04-29)
The tumour-suppressor gene p53 is inactivated in most human malignancies either by missense mutations or by binding to oncogenic proteins. In human soft tissue sarcomas, inactivation apparently results from MDM2 gene amplification. MDM2 is an oncogene product that may function
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