NE1004

Anti-Apolipoprotein E Mouse mAb (E6D7)

liquid, clone E6D7, Calbiochem^®

• Synonim(y): Anti-ApoE
• Kod UNSPSC: 12352203
• NACRES: NA.41

Właściwości

• pochodzenie biologiczne: mouse
• Poziom jakości: 100
• forma przeciwciała: culture supernatant
• rodzaj przeciwciała: primary antibodies
• klon: E6D7, monoclonal
• Postać: liquid
• nie zawiera: preservative
• reaktywność gatunkowa: human
• spodziewany brak reakcji z: mouse, rabbit
• producent / nazwa handlowa: Calbiochem^®
• warunki przechowywania: OK to freeze; avoid repeated freeze/thaw cycles
• izotyp: IgG1
• Warunki transportu: wet ice
• temp. przechowywania: −20°C
• docelowa modyfikacja potranslacyjna: unmodified
• informacje o genach: human ... APOE(348)

Opis

Opis ogólny

Anti-Apolipoprotein E, mouse monoclonal, clone E6D7, recognizes the ~35 kDa Apo E2, E3, and E4 in human serum and brain. It is validated for ELISA, WB, IP, and paraffin and frozen sections.

Mouse monoclonal antibody supplied as diluted culture supernatant. Recognizes the ~35 kDa E2, E3, and E4 isoforms of apolipoprotein E.

Recognizes the ~35 kDa E2, E3, and E4 isoforms of apolipoprotein E in human serum and brain.

Immunogen

Human

a synthetic peptide corresponding to amino acids surrounding the polymorphic amino acid position 158 of ApoE

Zastosowanie

ELISA (see comments)
Immunoblotting (1:100-1:1000)
Immunoprecipitation (see comments)
Frozen Sections (1:100-1:10,000)
Paraffin Sections (1:100-1:10,000, formic acid or heat pre-treatment required)

Opakowanie

Please refer to vial label for lot-specific concentration.

Ostrzeżenie

Toxicity: Standard Handling (A)

Postać fizyczna

Culture supernatant diluted in PBS.

Rekonstytucja

Following initial thaw, aliquot and freeze (-20°C).

Komentarz do analizy

Positive Control
Brain tissue, serum

Inne uwagi

Golabek, A.A., et al. 1996. J. Biol. Chem.271, 10602.

Recognizes the ~35 kDa E2, E3, and E4 isoforms of apolipoprotein E in human serum, but multiple bands are seen when used with brain tissue samples. Staining of formalin-fixed paraffin-embedded tissues may be enhanced by the pre-treatment of tissue with 70% formic acid for 10-30 min at room temperature or by heat pre-treatment using citrate buffer, pH 6.0. This antibody has also been reported to work for immunoprecipitation and ELISA. Antibody should be titrated for optimal results in individual systems.

Informacje prawne

CALBIOCHEM is a registered trademark of Merck KGaA, Darmstadt, Germany

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Informacja o środkach bezpieczeństwa

• Kod klasy składowania: 11 - Combustible Solids
• Klasa zagrożenia wodnego (WGK): WGK 1
• Temperatura zapłonu (°F): Not applicable
• Temperatura zapłonu (°C): Not applicable