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Key Documents

MABT83

Sigma-Aldrich

Anti-Aggrecan Antibody, clone 6B4

clone 6B4, from mouse

Synonim(y):

Aggrecan core protein, Cartilage-specific proteoglycan core protein, CSPCP, Chondroitin sulfate proteoglycan core protein 1, Chondroitin sulfate proteoglycan 1, Aggrecan core protein 2

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About This Item

Kod UNSPSC:
12352203
eCl@ss:
32160702
NACRES:
NA.41

pochodzenie biologiczne

mouse

Poziom jakości

forma przeciwciała

purified immunoglobulin

rodzaj przeciwciała

primary antibodies

klon

6B4, monoclonal

reaktywność gatunkowa

bovine

reaktywność gatunkowa (przewidywana na podstawie homologii)

human (based on 100% sequence homology)

metody

immunohistochemistry: suitable
western blot: suitable

izotyp

IgG1κ

numer dostępu NCBI

numer dostępu UniProt

Warunki transportu

wet ice

docelowa modyfikacja potranslacyjna

unmodified

informacje o genach

human ... ACAN(176)

Opis ogólny

Aggrecan (CSPG1, CSPCP, or CSPGCP) is a chondroitin-sulfate proteoglycan belonging to the family of letican proteins. Aggrecan is a large globar structure containing multiple domains including carbohydrate-recognition domains; complement binding protein domains; immunoglobulin domains; proteoglycan tandem repeats; and in some cases, EGF-like domains. It can be degraded by aggrecanase. The N-terminus of aggrecan binds ECM hyaluronan in the presence of the linking protein, and forms complex aggregates, whereas the C-terminus binds a number of other ECM proteins including tenascins, glycolipids, and fibulins. Aggrecan is a major component of cartilage tissue and it enables this tissue to withstand compression by maintaining the viso-elastic properties of cartilage. Aggrecan may also facilitate neural maturation and plasticity by contributing to the formation of perineuronal nets in the developing CNS.

Immunogen

Ovalbumin-conjugated linear peptide corresponding to human Aggrecan.

Zastosowanie

Anti-Aggrecan Antibody, clone 6B4 is an antibody against Aggrecan for use in Western Blotting & IHC.
Immunofluorescent Analysis: A representative lot was used by an independent laboratory in human femoral chondrocytes (Williams, R., et al. (2010). PLoS One. 5(10):e13246.).

Immunohistochemistry Analysis: A representative lot was used by an independent laboratory in human chartilage tissue (Williams, R., et al. (2010). PLoS One. 5(10):e13246.).
Research Category
Cell Structure
Research Sub Category
ECM Proteins

Jakość

Evaluated by Western Blot in A1D1 purified protein from bovine tissue lysate.

Western Blot Analysis: 1 µg/mL of this antibody detected Aggrecan in 10 µg of purified protein from bovine tissue lysate.

Opis wartości docelowych

~60 kDa observed. Uniprot describes three isoforms at ~250 kDa, ~246 kDa, and ~239 kDa Aggrecan is degraded by aggrecanase into a ~60 kDa protein and other higher molecular weight products (Lemons, M. L. et al. (2001). J Neurosci. 21(13):4772-4781. Forsang, A. J. et al. (1995). Biochem J. 310(Pt 1):337-343.).

Postać fizyczna

Format: Purified
Protein G Purified
Purified mouse monoclonal IgG1κ in buffer containing 0.1 M Tris-Glycine (pH 7.4), 150 mM NaCl with 0.05% sodium azide.

Przechowywanie i stabilność

Stable for 1 year at 2-8°C from date of receipt.

Komentarz do analizy

Control
A1D1 purified protein from bovine tissue lysate

Inne uwagi

Concentration: Please refer to the Certificate of Analysis for the lot-specific concentration.

Oświadczenie o zrzeczeniu się odpowiedzialności

Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.
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Kod klasy składowania

12 - Non Combustible Liquids

Klasa zagrożenia wodnego (WGK)

WGK 1

Temperatura zapłonu (°F)

Not applicable

Temperatura zapłonu (°C)

Not applicable


Certyfikaty analizy (CoA)

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Masz już ten produkt?

Dokumenty związane z niedawno zakupionymi produktami zostały zamieszczone w Bibliotece dokumentów.

Odwiedź Bibliotekę dokumentów

Identification and clonal characterisation of a progenitor cell sub-population in normal human articular cartilage.
Williams, Rebecca, et al.
Testing, 5, e13246-e13246 (2010)
Wen-Cheng Lo et al.
Life (Basel, Switzerland), 11(10) (2021-10-24)
Complications of diabetes mellitus (DM) range from acute to chronic conditions, leading to multiorgan disorders such as nephropathy, retinopathy, and neuropathy. However, little is known about the influence of DM on intervertebral disc degeneration (IVDD). Moreover, traditional surgical outcomes in
Navneet Kumar Dubey et al.
Aging and disease, 10(3), 483-496 (2019-06-06)
Diabetes mellitus (DM) is well-known to exert complications such as retinopathy, cardiomyopathy and neuropathy. However, in recent years, an elevated osteoarthritis (OA) complaints among diabetics have been observed, portending the risk of diabetic OA. Since formation of advanced glycation end
Xiaoyi Zhao et al.
Molecular therapy. Nucleic acids, 22, 832-845 (2020-11-25)
Knee osteoarthritis (KOA) is a highly prevalent disabling joint disease in aged people. Progressive cartilage degradation is the hallmark of KOA, but its deeper mechanism remains unclear. Substantial evidence indicates the importance of the synovium for joint homeostasis. The present
Hira Lal Goel et al.
Cell reports, 7(3), 747-761 (2014-04-29)
Although the α6β1 integrin has been implicated in the function of breast and other cancer stem cells (CSCs), little is known about its regulation and relationship to mechanisms involved in the genesis of CSCs. We report that a CD44(high)/CD24(low) population, enriched

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