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Key Documents

MABS2304

Sigma-Aldrich

Anti-ASGPR sH2a Antibody, clone B9

Synonim(y):

Asialoglycoprotein receptor 2;ASGP-R 2;C-type lectin domain family 4 member H2;Hepatic lectin H2;HL-2

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About This Item

Kod UNSPSC:
12352203
NACRES:
NA.43

pochodzenie biologiczne

mouse

Poziom jakości

forma przeciwciała

purified antibody

rodzaj przeciwciała

primary antibodies

klon

B9, monoclonal

masa cząsteczkowa

calculated mol wt 35.09 kDa

oczyszczone przez

using protein G

reaktywność gatunkowa

human

opakowanie

antibody small pack of 100 μg

metody

ELISA: suitable
immunoprecipitation (IP): suitable

izotyp

IgG3λ

sekwencja epitopowa

C-terminal

numer dostępu Protein ID

numer dostępu UniProt

temp. przechowywania

2-8°C

docelowa modyfikacja potranslacyjna

unmodified

informacje o genach

human ... ASGR2(433)

Opis ogólny

Asialoglycoprotein receptor 2 (UniProt: P07307; also known as ASGP-R 2, ASGPR 2, C-type lectin domain family 4 member H2, Hepatic lectin H2, HL-2) is encoded by the ASGR2 (also known as CLEC4H2) gene (Gene ID: 433) in human. ASGPR is a type II membrane glycoprotein that contains a cytoplasmic domain (aa 1-58), a transmembrane domain (aa 59-79), and an extracellular domain (aa 80-311). Its C-type lectin domain is localized in amino acids 176-302. ASGPR is expressed exclusively in hepatic parenchymal cells where it serves in clearance of asialoglycoproteins from the plasma. It mediates the endocytosis of plasma glycoproteins to which the terminal sialic acid residue on their complex carbohydrate moieties has been removed. The receptor recognizes terminal galactose and N-acetylgalactosamine units. After ligand binding to the receptor, the resulting complex is internalized and transported to a sorting organelle, where receptor and ligand are disassociated, and the receptor is returned to the cell membrane surface. A soluble form of ASGPR (~ 35 kDa) has also been described and is termed as sH2a that is formed by cleavage in the endoplasmic reticulum of its precursor. Levels of ASGPR sH2a are significantly reduced under conditions of liver cirrhosis and fibrosis. Clone B9 specifically detects the secreted sH2a form of ASGPR in human plasma and in NIH3T3 cells transfected with human ASGPR sH2a. (Ref.: Shenkman, M., et al. (2013). J. Biol. Chem. 288(4); 2167-2178; Benyair, R., et al. (2011). World J. Gastroenterol. 17(48); 5305-5309).

Specyficzność

Clone B9 is a mouse monoclonal antibody that detects Asialoglycoprotein receptor 2 (ASGPR2). It targets an epitope within 12 amino acids from the C-terminal region.

Immunogen

KLH-conjugated linear peptide corresponding to 12 amino acids from the C-terminal region of human Asialoglycoprotein receptor 2.

Zastosowanie

Quality Control Testing

Evaluated by ELISA with recombinant human ASGPR protein.

ELISA Analysis (ELISA): Various dilutions of this antibody detected recombinant human ASGPR sH2a protein.

Tested Applications

Immunoprecipitation Analysis: A representative lot immunoprecipitated ASGPR sH2a in Immunoprecipitation applications (Benyair, R., et al. (2011). World J Gastroenterol. 17(48):5305-9; Shenkman, M., et al. (2013). J Biol Chem. 288(4):2167-78).

ELISA Analysis: A representative lot detected ASGPR sH2a in ELISA applications (Benyair, R., et al. (2011). World J Gastroenterol. 17(48):5305-9).

Note: Actual optimal working dilutions must be determined by end user as specimens, and experimental conditions may vary with the end user.

Postać fizyczna

Purified mouse monoclonal antibody IgG3 in buffer containing 0.1 M Tris-Glycine (pH 7.4), 150 mM NaCl with 0.05% sodium azide.

Rekonstytucja

1.0 mg/mL. Please refer to guidance on suggested starting dilutions and/or titers per application and sample type.

Przechowywanie i stabilność

Stable for 1 year at +2°C to +8°C from date of receipt.

Inne uwagi

Concentration: Please refer to the Certificate of Analysis for the lot-specific concentration.

Oświadczenie o zrzeczeniu się odpowiedzialności

Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.
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Kod klasy składowania

12 - Non Combustible Liquids

Klasa zagrożenia wodnego (WGK)

WGK 1

Temperatura zapłonu (°F)

Not applicable

Temperatura zapłonu (°C)

Not applicable


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