MABS2304

Anti-ASGPR sH2a Antibody, clone B9

• Synonim(y): Asialoglycoprotein receptor 2;ASGP-R 2;C-type lectin domain family 4 member H2;Hepatic lectin H2;HL-2
• Kod UNSPSC: 12352203
• NACRES: NA.43

Właściwości

• pochodzenie biologiczne: mouse
• Poziom jakości: 200
• forma przeciwciała: purified antibody
• rodzaj przeciwciała: primary antibodies
• klon: B9, monoclonal
• masa cząsteczkowa: calculated mol wt 35.09 kDa
• oczyszczone przez: using protein G
• reaktywność gatunkowa: human
• opakowanie: antibody small pack of 100 μg
• metody: ELISA: suitable; immunoprecipitation (IP): suitable
• izotyp: IgG3λ
• sekwencja epitopowa: C-terminal
• numer dostępu Protein ID: NP_001172
• numer dostępu UniProt: P07307
• temp. przechowywania: 2-8°C
• docelowa modyfikacja potranslacyjna: unmodified
• informacje o genach: human ... ASGR2(433)

Opis

Opis ogólny

Asialoglycoprotein receptor 2 (UniProt: P07307; also known as ASGP-R 2, ASGPR 2, C-type lectin domain family 4 member H2, Hepatic lectin H2, HL-2) is encoded by the ASGR2 (also known as CLEC4H2) gene (Gene ID: 433) in human. ASGPR is a type II membrane glycoprotein that contains a cytoplasmic domain (aa 1-58), a transmembrane domain (aa 59-79), and an extracellular domain (aa 80-311). Its C-type lectin domain is localized in amino acids 176-302. ASGPR is expressed exclusively in hepatic parenchymal cells where it serves in clearance of asialoglycoproteins from the plasma. It mediates the endocytosis of plasma glycoproteins to which the terminal sialic acid residue on their complex carbohydrate moieties has been removed. The receptor recognizes terminal galactose and N-acetylgalactosamine units. After ligand binding to the receptor, the resulting complex is internalized and transported to a sorting organelle, where receptor and ligand are disassociated, and the receptor is returned to the cell membrane surface. A soluble form of ASGPR (~ 35 kDa) has also been described and is termed as sH2a that is formed by cleavage in the endoplasmic reticulum of its precursor. Levels of ASGPR sH2a are significantly reduced under conditions of liver cirrhosis and fibrosis. Clone B9 specifically detects the secreted sH2a form of ASGPR in human plasma and in NIH3T3 cells transfected with human ASGPR sH2a. (Ref.: Shenkman, M., et al. (2013). J. Biol. Chem. 288(4); 2167-2178; Benyair, R., et al. (2011). World J. Gastroenterol. 17(48); 5305-5309).

Specyficzność

Clone B9 is a mouse monoclonal antibody that detects Asialoglycoprotein receptor 2 (ASGPR2). It targets an epitope within 12 amino acids from the C-terminal region.

Immunogen

KLH-conjugated linear peptide corresponding to 12 amino acids from the C-terminal region of human Asialoglycoprotein receptor 2.

Zastosowanie

Quality Control Testing

Evaluated by ELISA with recombinant human ASGPR protein.

ELISA Analysis (ELISA): Various dilutions of this antibody detected recombinant human ASGPR sH2a protein.

Tested Applications

Immunoprecipitation Analysis: A representative lot immunoprecipitated ASGPR sH2a in Immunoprecipitation applications (Benyair, R., et al. (2011). World J Gastroenterol. 17(48):5305-9; Shenkman, M., et al. (2013). J Biol Chem. 288(4):2167-78).

ELISA Analysis: A representative lot detected ASGPR sH2a in ELISA applications (Benyair, R., et al. (2011). World J Gastroenterol. 17(48):5305-9).

Note: Actual optimal working dilutions must be determined by end user as specimens, and experimental conditions may vary with the end user.

Postać fizyczna

Purified mouse monoclonal antibody IgG3 in buffer containing 0.1 M Tris-Glycine (pH 7.4), 150 mM NaCl with 0.05% sodium azide.

Rekonstytucja

1.0 mg/mL. Please refer to guidance on suggested starting dilutions and/or titers per application and sample type.

Przechowywanie i stabilność

Stable for 1 year at +2°C to +8°C from date of receipt.

Inne uwagi

Concentration: Please refer to the Certificate of Analysis for the lot-specific concentration.

Oświadczenie o zrzeczeniu się odpowiedzialności

Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.

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Informacja o środkach bezpieczeństwa

• Kod klasy składowania: 12 - Non Combustible Liquids
• Klasa zagrożenia wodnego (WGK): WGK 1
• Temperatura zapłonu (°F): Not applicable
• Temperatura zapłonu (°C): Not applicable