MABS1163

Anti-CSF-1R/CD115 Antibody clone FER216

clone FER216, from mouse

• Synonim(y): Macrophage colony-stimulating factor 1 receptor, CD115, CSF-1 receptor, CSF-1-R, CSF-1R, M-CSF-R, Proto-oncogene c-Fms
• Kod UNSPSC: 12352203
• eCl@ss: 32160702
• NACRES: NA.41

Właściwości

• pochodzenie biologiczne: mouse
• Poziom jakości: 100
• forma przeciwciała: purified immunoglobulin
• rodzaj przeciwciała: primary antibodies
• klon: FER216, monoclonal
• reaktywność gatunkowa: human
• metody: western blot: suitable
• izotyp: IgG1κ
• numer dostępu NCBI: NP_005202
• numer dostępu UniProt: P07333
• Warunki transportu: wet ice
• docelowa modyfikacja potranslacyjna: unmodified
• informacje o genach: human ... CSF1R(1436)

Opis

Opis ogólny

Macrophage colony-stimulating factor 1 receptor (EC 2.7.10.1; UniProt P07333; also known as CD115, CSF-1 receptor, CSF-1-R, CSF-1R, M-CSF-R, Proto-oncogene c-Fms) is encoded by the CSF1R (also known as CSFR, FMS, HDLS) gene (Gene ID 1436) in human. CSF1R is a CSF1/PDGF family receptor tyrosine kinase that mediates macrophage colony stimulating factor 1 (M-CSF1) and IL-34 signaling by acting as the receptor for these two cytokines. CSF1R plays a key role in the differentiation, recruitment and activation of tissue macrophages. Tumor-associated macrophages (TAMs) have been associated with survival in classic Hodgkin lymphoma (cHL) and other lymphoma types. Hyperphosphorylated CSF1R is found in cHL tissue and highlights the importance of CSF1/CSF1R signaling in the recruitment of TAMs. CSF1R is initially produced with a signal peptide sequence (a.a. 1-19), the removal of which yields the mature type I single transmembrane protein with an extracellular region (a.a. 20-517), a transmembrane segment (a.a. a.a. 518-538), and a cytoplasmic region (a.a. 539-972) that contains the kinase domain (a.a. 582-910).

Specyficzność

Clone FER216 reacts with the extracellular domain of human CSFR1, but not the extracellular domain of human FGFR1. Clone FER216 detected the ~100 kDa human CSFR1 in nomral tonsil and Hodgkin lymphoma (cHL) tissue extracts, as well as an additional ~150 kDa phosphorylated CSFR1 band in cHL tissue extracts (Martín-Moreno, A.M., et al. (2015). PLoS One. 2015 Jun 12;10(6):e0125203).

Immunogen

Epitope: Extracellular domain.

Human IgG1 Fc-tagged recombinant human CSF-1R/CD115 extracellular domain.

Zastosowanie

Anti-CSF-1R/CD115 Antibody clone FER216 is an antibody against CSF-1R for use in Western Blotting.

Research Category
Signaling

Research Sub Category
Cytokines & Cytokine Receptors

Western Blotting Analysis: 1.0 µg/mL from a representative lot detected CSF-1R/CD115 in 10 µg of human tonsil tissue lysate.

Jakość

Evaluated by Western Blotting in human colon tissue lysate.

Western Blotting Analysis: 0.5 µg/mL of this antibody detected CSF-1R/CD115 in 10 µg of human colon tissue lysate.

Opis wartości docelowych

~107 kDa observed. Uncharacterized band(s) may appear in some lysates.

Postać fizyczna

Format: Purified

Protein G Purified

Purified mouse monoclonal IgG1κ antibody in buffer containing 0.1 M Tris-Glycine (pH 7.4), 150 mM NaCl with 0.05% sodium azide.

Przechowywanie i stabilność

Stable for 1 year at 2-8°C from date of receipt.

Inne uwagi

Concentration: Please refer to lot specific datasheet.

Oświadczenie o zrzeczeniu się odpowiedzialności

Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.

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Informacja o środkach bezpieczeństwa

• Kod klasy składowania: 12 - Non Combustible Liquids
• Klasa zagrożenia wodnego (WGK): WGK 1
• Temperatura zapłonu (°F): Not applicable
• Temperatura zapłonu (°C): Not applicable