MAB2166
Anti-Huntingtin Protein Antibody, a.a. 181-810, clone 1HU-4C8
ascites fluid, clone 1HU-4C8, Chemicon®
Synonim(y):
Huntingtin, Huntington′s Disease Protein, HD Protein
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About This Item
Kod UNSPSC:
12352203
eCl@ss:
32160702
NACRES:
NA.41
Polecane produkty
pochodzenie biologiczne
mouse
Poziom jakości
forma przeciwciała
ascites fluid
rodzaj przeciwciała
primary antibodies
klon
1HU-4C8, monoclonal
reaktywność gatunkowa
rat, rabbit
reaktywność gatunkowa (przewidywana na podstawie homologii)
mouse, human, hamster, monkey
producent / nazwa handlowa
Chemicon®
metody
ELISA: suitable
immunocytochemistry: suitable
immunohistochemistry: suitable (paraffin)
immunoprecipitation (IP): suitable
western blot: suitable
izotyp
IgG1κ
numer dostępu NCBI
numer dostępu UniProt
Warunki transportu
dry ice
docelowa modyfikacja potranslacyjna
unmodified
informacje o genach
human ... HTT(3064) , SLC6A4(6532)
Opis ogólny
Huntington disease (HD) is a hereditary, progressive, neurodegenerative ailment characterized by personality changes, motor impairment and subcortical dementia. The molecular basis of the disease involves the expansion of the trinucleotide CAG, coding for polyglutamine in the first exon of a chromosome four gene (4p16.3), which normally produces a widely expressed 3136 a.a. (~350 kDa) protein huntingtin with unclear function. The protein is found in the perinuclear region along with microtubules, and in the centrosomal region along with gamma-tubulin. Huntingtin is necessary for neuronal survival and is involved in synaptic vesicle trafficking, microtubule binding and may also have a role in apoptosis. In the HD condition, neuronal cells with the mutant form of huntingtin possess intranuclear aggregations of the N-terminal fragment, causing damaging inclusions in perinuclear locations and striatal neuron cell death. Wild-type huntington and anti-huntingtin reduce aggregation and cellular toxicity of the mutant huntingtin form in mammalian cell models of HD. Huntingtin is known to interact with GAPDH, HAP-1, SP1 and TAFII130.
Specyficzność
Huntingtin Protein. No detectable cross reactivity to other proteins by Western blot.
Immunogen
Epitope: a.a. 181-810
Huntingtin fragment from a.a. 181 to 810 as a fusion protein.
Zastosowanie
Anti-Huntingtin Protein Antibody, a.a. 181-810, clone 1HU-4C8 is an antibody against Huntingtin Protein for use in ELISA, IC, IH(P), IP & WB.
ELISA:
A 1:500-1:5,000 dilution of a previous lot was used on ELISA.
Immunohistochemistry:
A 1:500-1:5,000 dilution from a previous lot was used on frozen and microwave oven treated paraffin sections (human tissue).
Immunocytochemistry:
1:500-1:5,000 on a previous lot was used on transfected cells.
Immunoprecipitation:
A 1:500-1:5,000 dilution of a previous lot was used on immunoprecipitation.
Western blot:
1:500-1:5,000. Should detect a band migrating at approximately 350-400 kDa by Western blot (Nature Genetics 10:104-110.).
Optimal working dilutions must be determined by the end user.
A 1:500-1:5,000 dilution of a previous lot was used on ELISA.
Immunohistochemistry:
A 1:500-1:5,000 dilution from a previous lot was used on frozen and microwave oven treated paraffin sections (human tissue).
Immunocytochemistry:
1:500-1:5,000 on a previous lot was used on transfected cells.
Immunoprecipitation:
A 1:500-1:5,000 dilution of a previous lot was used on immunoprecipitation.
Western blot:
1:500-1:5,000. Should detect a band migrating at approximately 350-400 kDa by Western blot (Nature Genetics 10:104-110.).
Optimal working dilutions must be determined by the end user.
Research Category
Neuroscience
Neuroscience
Research Sub Category
Neurodegenerative Diseases
Neurodegenerative Diseases
Jakość
Routinely evaluated by Western Blot on rat brain lysates.
Western Blot Analysis:
1:1000 dilution of this lot detected huntingtin protein on 10 μg of rat brain lysates.
Western Blot Analysis:
1:1000 dilution of this lot detected huntingtin protein on 10 μg of rat brain lysates.
Opis wartości docelowych
~ 350-400 kDa
Postać fizyczna
Ascites mouse monoclonal IgG1κ liquid containing no preservative
Unpurified
Przechowywanie i stabilność
Stable for 1 year at -20ºC in undiluted aliquots from date of receipt.
Handling Recommendations: Upon receipt, and prior to removing the cap, centrifuge the vial and gently mix the solution. Aliquot into microcentrifuge tubes and store at -20°C. Avoid repeated freeze/thaw cycles, which may damage IgG and affect product performance.
Handling Recommendations: Upon receipt, and prior to removing the cap, centrifuge the vial and gently mix the solution. Aliquot into microcentrifuge tubes and store at -20°C. Avoid repeated freeze/thaw cycles, which may damage IgG and affect product performance.
Komentarz do analizy
Control
Normal human cerebral cortex lysate
Normal human cerebral cortex lysate
Inne uwagi
Concentration: Please refer to the Certificate of Analysis for the lot-specific concentration.
Informacje prawne
CHEMICON is a registered trademark of Merck KGaA, Darmstadt, Germany
Oświadczenie o zrzeczeniu się odpowiedzialności
Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.
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polecane
Kod klasy składowania
12 - Non Combustible Liquids
Klasa zagrożenia wodnego (WGK)
WGK 2
Temperatura zapłonu (°F)
Not applicable
Temperatura zapłonu (°C)
Not applicable
Certyfikaty analizy (CoA)
Poszukaj Certyfikaty analizy (CoA), wpisując numer partii/serii produktów. Numery serii i partii można znaleźć na etykiecie produktu po słowach „seria” lub „partia”.
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Proceedings of the National Academy of Sciences of the USA null
C L Benn et al.
Neuroscience, 147(2), 354-372 (2007-06-05)
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L Cai et al.
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Produkty
Immunofluorescencja wykorzystuje cząsteczki fluorescencyjne sprzężone z przeciwciałami do lokalizacji białek, potwierdzania modyfikacji i wizualizacji kompleksów białkowych.
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