MABN869

Anti-DARPP-32 Antibody, clone 9B6.1

clone 9B6.1, from mouse

• Synonim(y): Protein phosphatase 1 regulatory subunit 1B, DARPP-32, Dopamine- and cAMP-regulated neuronal phosphoprotein
• Kod UNSPSC: 12352203
• eCl@ss: 32160702
• NACRES: NA.41

Właściwości

• pochodzenie biologiczne: mouse
• Poziom jakości: 100
• forma przeciwciała: purified immunoglobulin
• rodzaj przeciwciała: primary antibodies
• klon: 9B6.1, monoclonal
• reaktywność gatunkowa: human, rat
• metody: immunohistochemistry: suitable; western blot: suitable
• izotyp: IgG1κ
• numer dostępu NCBI: NP_001229393
• numer dostępu UniProt: Q9UD71
• Warunki transportu: wet ice
• docelowa modyfikacja potranslacyjna: unmodified
• informacje o genach: human ... PPP1R1B(84152)

Opis

Opis ogólny

Protein phosphatase 1 regulatory subunit 1B (EC 3.1.3.16; UniProt Q9UD71; also known as DARPP-32, Dopamine- and cAMP-regulated neuronal phosphoprotein 32) is encoded by the PPP1R1B (also known as DARPP32) gene (Gene ID 84152) in human. DARPP-32 is a PKA substrate that, upon phosphorylation on Thr34 by PKA, functions as a protein-phosphatase 1 (PP1) inhibitor. DARPP-32 N-terminal a.a. 8-11 (KIQF) is essential for PPI binding. DARPP-32 is an important mediator of dopamine receptor-dependent NMDA receptor activation. Dopamine receptor activation leads to cAMP induction, which activates cellular PKA activity and subsequent DARPP-32 Thr34 phosphorylation. PP1 inhibition by the Thr34 phosphorylated DARPP-32 in turn results in an enhnaced NMDA receptor phosphorylation. Calcium/calmodulin-dependent protein phosphatase 2B (PP2B or calcineurin) can reverse DARPP-32 Thr34 phosphorylation and negatively regulate its PP1 inhibitory activity. DARPP-32 is also known to be phosphorylated on Ser137 by casein kinase I (CKI), on Ser102 by CKII, and on Thr75 by Cdk5. Ser137 phosphorylation prevents PP2B from dephosphorylating pSer34, and Ser102 phosphorylation promotes Thr34 phosphorylation by PKA. Thr75 phosphorylated DARPP-32 is reported to exhibit PKA inhibitory activity in vitro.

Specyficzność

Expected to react with both spliced isoforms of human DARPP-32 (UniProt Q9UD71-1 & Q9UD71-2).

Immunogen

Epitope: C-terminal half.

GST-tagged recombinant protein corresponding to the C-terminal half of human DARPP-32.

Zastosowanie

Research Category
Neuroscience

Research Sub Category
Signaling Neuroscience

This Anti-DARPP-32 Antibody, clone 9B6.1 is validated for use in Western Blotting, Immunohistochemistry for the detection of DARPP-32.

Western Blotting Analysis: 1.0 µg/mL from a representative lot detected DARPP-32 in 10 µg of rat brain tissue lysate.
Immunohistochemistry Analysis: A 1:1000 dilution from a representative lot detected DARPP-32 in human cerebral cortex and human testis tissue.

Jakość

Evaluated by Western Blotting in human brain tissue lysate.

Western Blotting Analysis: 1.0 µg/mL of this antibody detected DARPP-32 in 10 µg of human brain tissue lysate.

Opis wartości docelowych

~32/30 kDa observed. Target band(s) appears larger than the caluclated molecular weights of 22.96 kDa (isoform 1) and 18.74 kDa (Isoform 2).

Postać fizyczna

Format: Purified

Protein G Purified

Purified mouse monoclonal IgG1κ antibody in buffer containing 0.1 M Tris-Glycine (pH 7.4), 150 mM NaCl with 0.05% sodium azide.

Przechowywanie i stabilność

Stable for 1 year at 2-8°C from date of receipt.

Inne uwagi

Concentration: Please refer to lot specific datasheet.

Oświadczenie o zrzeczeniu się odpowiedzialności

Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.

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Informacja o środkach bezpieczeństwa

• Kod klasy składowania: 12 - Non Combustible Liquids
• Klasa zagrożenia wodnego (WGK): WGK 1
• Temperatura zapłonu (°F): Not applicable
• Temperatura zapłonu (°C): Not applicable