MABN486

Anti-Co-Rest/RCOR1 Antibody, clone K72/8

clone K72/8, from mouse

• Synonim(y): REST corepressor 1, Protein CoREST
• Kod UNSPSC: 12352203
• eCl@ss: 32160702
• NACRES: NA.41

Właściwości

• pochodzenie biologiczne: mouse
• Poziom jakości: 100
• forma przeciwciała: purified immunoglobulin
• rodzaj przeciwciała: primary antibodies
• klon: K72/8, monoclonal
• reaktywność gatunkowa: rat, human, mouse
• metody: immunocytochemistry: suitable; immunohistochemistry: suitable; western blot: suitable
• izotyp: IgG1
• numer dostępu NCBI: NP_055971
• numer dostępu UniProt: Q9UKL0
• Warunki transportu: wet ice
• docelowa modyfikacja potranslacyjna: unmodified
• informacje o genach: human ... RCOR1(23186)

Opis

Opis ogólny

REST corepressor 1(RCOR1, CoREST) is an essential component of the BHC corepressor complex that represses transcription of neuron-specific genes in non-neuronal cells by deacetylating and demethylating specific sites on histones, thereby acting as a chromatin modifier. RCOR1 also promotes the demethylase activity of KDM1A on core histones and nucleosomal substrates and plays a central role in demethylation of Lys-4 of histone H3. Furthermore, RCOR1 is a component of a RCOR/GFI/KDM1A/HDAC complex that suppresses a number of genes implicated in multilineage blood cell development and controls hematopoietic differentiation. RCOR1 is ubiquitously expressed and is primarily found in the nucleus, but is partially translocated into the cytoplasm upon HSV-1 infection.

Specyficzność

This antibody may cross react with human RCOR3 based upon 70% sequence homology.

Immunogen

Recombinant protein corresponding to human Co-Rest/RCOR1.

Zastosowanie

Anti-Co-Rest/RCOR1 Antibody, clone K72/8 is a highly specific mouse monoclonal antibody, that targets CoRest & has been tested in western blotting, IHC & ICC.

Immunohistochemistry Analysis: A 1:250 dilution from a representative lot detected Co-Rest/RCOR1 in human stomach tissue.
Immunocytochemistry Analysis: A representative lot detected Co-Rest/RCOR1 in COS-1 cells expressing Myc-tagged Co-Rest. (Prof. J. Trimmer, University of California, Davis.).

Research Category
Neuroscience

Research Sub Category
Developmental Signaling

Jakość

Evaluated by Western Blotting in Jurkat cell lysate.

Western Blotting Analysis: 1.0 µg/mL of this antibody detected Co-Rest/RCOR1 in 10 µg of Jurkat cell lysate.

Opis wartości docelowych

~ 65 kDa observed. The calculated molecular weight is 53 kDa, however Co-Rest/RCOR1 has been shown as a ~65-70 kDa band in western blots (You, A., et al. (2001). National Academy of Sciences. 98(4):1454-1458)(Min, G. L., et al. (2005). Nature. 437:432-435).

Postać fizyczna

Format: Purified

Protein G Purified

Purified mouse monoclonal IgG1 in buffer containing 0.1 M Tris-Glycine (pH 7.4), 150 mM NaCl with 0.05% sodium azide.

Przechowywanie i stabilność

Stable for 1 year at 2-8°C from date of receipt.

Komentarz do analizy

Control
Jurkat cell lysate

Inne uwagi

Concentration: Please refer to the Certificate of Analysis for the lot-specific concentration.

Oświadczenie o zrzeczeniu się odpowiedzialności

Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.

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Informacja o środkach bezpieczeństwa

• Kod klasy składowania: 12 - Non Combustible Liquids
• Klasa zagrożenia wodnego (WGK): WGK 1
• Temperatura zapłonu (°F): Not applicable
• Temperatura zapłonu (°C): Not applicable