MABN2257

Anti-Nudel (NDEL1) Antibody, clone 15D5.1

clone 15D5.1, from mouse

• Synonim(y): Nuclear distribution protein nudE-like 1, Protein Nudel, Mitosin-associated protein 1
• Kod UNSPSC: 12352203
• eCl@ss: 32160702
• NACRES: NA.41

Właściwości

• pochodzenie biologiczne: mouse
• Poziom jakości: 100
• forma przeciwciała: purified antibody
• rodzaj przeciwciała: primary antibodies
• klon: 15D5.1, monoclonal
• reaktywność gatunkowa: mouse, human
• metody: immunohistochemistry: suitable (paraffin); western blot: suitable
• izotyp: IgG1κ
• numer dostępu NCBI: NP_110435
• numer dostępu UniProt: Q9GZM8
• Warunki transportu: ambient
• docelowa modyfikacja potranslacyjna: unmodified
• informacje o genach: human ... NDEL1(81565)

Opis

Opis ogólny

Nuclear distribution protein nudE-like 1 (UniProt: Q9GZM8; also known as Protein Nudel, Mitosin-associated protein 1) is encoded by the NDEL1 (also known as EOPA, MITAP1, NUDEL) gene (Gene ID: 81565) in human. Nudel protein belongs to the nude family and is expressed in brain, heart, kidney, liver, lung, pancreas, placenta and skeletal muscle. Its expression is shown to peak during mitosis. Nudel is undergoes phosphorylation during mitosis. Three isoforms of Nudel have been reported that are produced by alternative splicing. Nudel is required for organization of the cellular microtubule array and microtubule anchoring at the centrosome. It may regulate microtubule organization at least in part by targeting the microtubule severing protein KATNA1 to the centrosome and is shown to positively regulate the activity of the minus-end directed microtubule motor protein dynein. It enhances dynein-mediated microtubule sliding by targeting dynein to the microtubule plus ends and is shown to be essential for several dynein- and microtubule-dependent processes, such as the maintenance of Golgi integrity, the centripetal motion of secretory vesicles and the coupling of the nucleus and centrosome. Nudel is required during brain development for the migration of newly formed neurons from the ventricular/subventricular zone toward the cortical plate and also plays a role in the regulation of neurite outgrowth. It localizes to the cell body of the motor neurons and colocalizes with assembled neurofilaments within axonal processes.

Specyficzność

Clone 15D5.1 detects Nuclear distribution protein nudE-like 1 (Nudel) in human and murine cells. It targets an epitope within 101 amino acids from the N-terminal region.

Immunogen

GST/His-taged recombinant fragment corresponding to 101 amino acids from the N-terminal region of human Nuclear distribution protein nudE-like 1 (Nudel).

Zastosowanie

Anti-Nudel (NDEL1), clone 15D5.1, Cat. No. MABN2257, is a highly specific mouse monoclonal antibody that targets Nuclear distribution protein nudE-like 1 (Nudel) and has been tested for use in Immunohistochemistry (Paraffin) and Western Blotting.

Immunohistochemistry Analysis: A 1:50 dilution from a representative lot detected Nuclear distribution protein nudE-like 1 (Nudel) in human colon, human cerebral cortex, and human tonsil tissues.

Research Category
Neuroscience

Jakość

Evaluated by Western Blotting in mouse brain tissue lysate.

Western Blotting Analysis: 1 µg/mL of this antibody detected Nuclear distribution protein nudE-like 1 (Nudel) in 10 µg of mouse brain tissue lysate.

Opis wartości docelowych

~40 kDa observed; 38.38 kda calculated. Uncharacterized bands may be observed in some lysate(s).

Postać fizyczna

Format: Purified

Protein G purified

Purified mouse monoclonal antibody IgG1 in buffer containing 0.1 M Tris-Glycine (pH 7.4), 150 mM NaCl with 0.05% sodium azide.

Przechowywanie i stabilność

Stable for 1 year at 2-8°C from date of receipt.

Inne uwagi

Concentration: Please refer to lot specific datasheet.

Oświadczenie o zrzeczeniu się odpowiedzialności

Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.

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Informacja o środkach bezpieczeństwa

• Kod klasy składowania: 12 - Non Combustible Liquids
• Klasa zagrożenia wodnego (WGK): WGK 1
• Temperatura zapłonu (°F): does not flash
• Temperatura zapłonu (°C): does not flash