MABN1844

Anti-Aldolase C Antibody, C-Term, clone 1A1

clone 1A1, from mouse

• Synonim(y): Fructose-bisphosphate aldolase C, Brain-type aldolase, Aldolase C, C-Term
• Kod UNSPSC: 12352203
• eCl@ss: 32160702
• NACRES: NA.41

Właściwości

• pochodzenie biologiczne: mouse
• Poziom jakości: 100
• forma przeciwciała: purified immunoglobulin
• rodzaj przeciwciała: primary antibodies
• klon: 1A1, monoclonal
• reaktywność gatunkowa: human, rat
• metody: immunocytochemistry: suitable; western blot: suitable
• izotyp: IgG1κ
• numer dostępu NCBI: NP_005156
• numer dostępu UniProt: P09972
• Warunki transportu: dry ice
• docelowa modyfikacja potranslacyjna: unmodified
• informacje o genach: human ... ALDOC(230)

Opis

Opis ogólny

Fructose-bisphosphate aldolase C (EC 4.1.2.13; UniProt P09972; also known as Aldolase 3, Brain-type aldolasefructoaldolase C, Fructose-1,6-biphosphate triosephosphate lyase) is encoded by the ALDOC (also known as ALDC) gene (Gene ID 230) in human. Aldolases are glycolytic enzymes that catalyze the reversible aldol cleavage of fructose 1,6-bisphosphate and fructose-1-phosphate to dihydroxyacetone phosphate and glyceraldehyde 3-phosphate. Three aldolase genes (ALDOA, ALDOB, ALDOC) are found in mammals that encode aldolase A, B, and C. Aldolase A is generally considered to be a muscle enzyme, aldolase B a liver enzyme, while aldolase C shows tissue-specific expression in adult brain with low activity in fetal tissues. Aldolase C expression is found in gray matter astrocytes and cells of the pia mater. Aldolase A and C mRNA expressions are mutually exclusive in murine central nervous system by in situ hybridization analysis, with aldolase A mRNA being detected in neurons and aldolase C in astrocytes, Purkinje cells, and some cells of the pia mater. Aldolase C can therefore be a useful marker of astrocytes, although Purkinje cells of the cerebellum also contain high levels of aldolase C.

Immunogen

Epitope: Near C-terminus; 23 amino acids of aldolase C protein

Recombinant protein corresponding to human Aldolase C near the C-terminus.

Zastosowanie

This Anti-Aldolase C Antibody, C-Term, clone 1A1 is validated for use in Western Blotting, Immunocytochemistry for the detection of Aldolase C.

Western Blotting Analysis: 0.5 µg/mL from a representative lot detected Aldolase C in 10 µg of human brain tissue lysate.
Immunocytochemistry: A representative lot selectively stained Aldolase C-positive astrocytes in mixed neuron/glial cultures.

Jakość

Evaluated by Western Blotting in rat brain tissue lysate.

Western Blotting Analysis: 0.5 µg/mL of this antibody detected Aldolase C in 10 µg of rat brain tissue lysate.

Opis wartości docelowych

~39 kDa observed

Postać fizyczna

Format: Purified

Purified mouse monoclonal IgG1κ antibody in buffer containing PBS with 0.05% sodium azide with 50% glycerol.

Inne uwagi

Concentration: Please refer to lot specific datasheet.

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Informacja o środkach bezpieczeństwa

• Kod klasy składowania: 10 - Combustible liquids
• Klasa zagrożenia wodnego (WGK): WGK 2
• Temperatura zapłonu (°F): Not applicable
• Temperatura zapłonu (°C): Not applicable