MABF949

Anti-Rabies Virus Antibody, clone 1112-1

clone 1112-1, from mouse

• Kod UNSPSC: 12352203
• eCl@ss: 32160702
• NACRES: NA.41

Właściwości

• pochodzenie biologiczne: mouse
• Poziom jakości: 100
• forma przeciwciała: purified immunoglobulin
• rodzaj przeciwciała: primary antibodies
• klon: 1112-1, monoclonal
• reaktywność gatunkowa: rabies virus
• reaktywność gatunkowa (przewidywana na podstawie homologii): all
• opakowanie: antibody small pack of 25 μg
• metody: flow cytometry: suitable; neutralization: suitable
• izotyp: IgG1κ; IgG1
• numer dostępu UniProt: P03524
• Warunki transportu: ambient
• docelowa modyfikacja potranslacyjna: unmodified

Opis

Opis ogólny

Glycoprotein of Evelyn Rokitniki Abelseth (ERA) SAD derived Rabies virus strain (UniProt: P03524) is encoded by the G gene. It belongs to the lyssavirus glycoprotein of the Rhabdoviridae family. This glycoprotein is a single pass type I virion membrane protein that helps in attaching the virus to host cellular receptor, inducing endocytosis of the virion. In the endosome, the acidic pH induces conformational changes in the glycoprotein trimer, which trigger fusion between virus and cell membrane. It has been shown that the muscular form of the nicotinic acetylcholine receptor (nAChR), the neuronal cell adhesion molecule (N-CAM), and the p75 neurotrophin receptor (p75NTR) bind glycoprotein and facilitate rabies virus entry into cells. Arg352 of Glycoprotein is highly involved in rabies virus pathogenicity and mutation at this site dramatically attenuates the virus.

Glycoprotein of Evelyn Rokitniki Abelseth (ERA) SAD derived Rabies virus strain (UniProt: P03524) is encoded by the G gene. It belongs to the lyssavirus glycoprotein of the Rhabdoviridae family. This glycoprotein is a single pass type I virion membrane protein that helps in attaching the virus to host cellular receptor, inducing endocytosis of the virion. In the endosome, the acidic pH induces conformational changes in the glycoprotein trimer, which trigger fusion between virus and cell membrane. It has been shown that the muscular form of the nicotinic acetylcholine receptor (nAChR), the neuronal cell adhesion molecule (N-CAM), and the p75 neurotrophin receptor (p75NTR) bind glycoprotein and facilitate rabies virus entry into cells. Arg352 of Glycoprotein is highly involved in rabies virus pathogenicity and mutation at this site dramatically attenuates the virus.

Specyficzność

Clone 1112-1 detects glycoprotein of ERA strain of rabies virus in L929 cells that express rabies protein.

Clone 1112-1 detects glycoprotein of ERA strain of rabies virus in L929 cells that express rabies protein.

Immunogen

Glycoprotein of ERA (Evelyn Rokitniki Abelseth) strain Rabies virus.

Glycoprotein of ERA (Evelyn Rokitniki Abelseth) strain Rabies virus.

Zastosowanie

Anti-Rabies Virus, clone 1112-1, Cat. No. MABF949, is a mouse monoclonal antibody that detects glycoprotein of strain ERA rabbies virus. It has been tested for use in Flow Cytometry and for Neutralizing.

Neutralizing Analysis: A representative lot neutralized Rabies Virus (Bunschoten, H., et. al. (1989). J Gen Virol. 70 ( Pt 2):291-8; Dietzchold, B., et. al. (1992). Proc Natl Acad Sci USA. 89(15):7252-6; Muller, T., et. al. (2009). PLoS Neql Trop Dis. 3(11):e542; Schumacher, C.L., et. al. (1989). J Clin Invest. 84(3):971-5).

Neutralizing Analysis: A representative lot neutralized Rabies Virus (Bunschoten, H., et. al. (1989). J Gen Virol. 70 ( Pt 2):291-8; Dietzchold, B., et. al. (1992). Proc Natl Acad Sci USA. 89(15):7252-6; Muller, T., et. al. (2009). PLoS Neql Trop Dis. 3(11):e542; Schumacher, C.L., et. al. (1989). J Clin Invest. 84(3):971-5).

Research Category
Inflammation & Immunology

Jakość

Evaluated by Flow Cytometry in L929 expressing rabies protein.

Flow Cytometry Analysis: 1 µg of this antibody detected Rabies Virus in one million L929 cells expressing rabies protein.

Opis wartości docelowych

58.66 kDa calculated.

Postać fizyczna

Format: Purified

Protein G purified

Purified mouse monoclonal antibody IgG1 in PBS without azide.

Przechowywanie i stabilność

Stable for 1 year at -20°C from date of receipt. Handling Recommendations: Upon receipt and prior to removing the cap, centrifuge the vial and gently mix the solution. Aliquot into microcentrifuge tubes and store at -20°C. Avoid repeated freeze/thaw cycles, which may damage IgG and affect product performance.

Inne uwagi

Concentration: Please refer to lot specific datasheet.

Oświadczenie o zrzeczeniu się odpowiedzialności

Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.

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Informacja o środkach bezpieczeństwa

• Kod klasy składowania: 12 - Non Combustible Liquids
• Klasa zagrożenia wodnego (WGK): WGK 2
• Temperatura zapłonu (°F): Not applicable
• Temperatura zapłonu (°C): Not applicable