MABF299

Anti-CCR2 Antibody, clone 8C3.1

clone 8C3.1, from mouse

• Synonim(y): C-C chemokine receptor type 2, C-C CKR-2, CC-CKR-2, CCR-2, CCR2, CD192, MCP-1-R, Monocyte chemoattractant protein 1 receptor
• Kod UNSPSC: 12352203
• eCl@ss: 32160702
• NACRES: NA.41
• klon: 8C3.1, monoclonal
• application: FACS; WB
• reaktywność gatunkowa: human
• metody: flow cytometry: suitable; western blot: suitable

Właściwości

• pochodzenie biologiczne: mouse
• Poziom jakości: 100
• forma przeciwciała: purified immunoglobulin
• rodzaj przeciwciała: primary antibodies
• klon: 8C3.1, monoclonal
• reaktywność gatunkowa: human
• metody: flow cytometry: suitable; western blot: suitable
• izotyp: IgG2aκ
• numer dostępu NCBI: NP_001116513
• numer dostępu UniProt: P41597
• Warunki transportu: ambient
• docelowa modyfikacja potranslacyjna: unmodified
• informacje o genach: human ... CCR2 (729230)

Opis

Opis ogólny

C-C chemokine receptor type 2 (UniProt P41597; also known as C-C CKR-2, CC-CKR-2, CCR-2, CCR2, CD192, MCP-1-R, Monocyte chemoattractant protein 1 receptor) is encoded by the CCR2 (also known as CMKBR2) gene (Gene ID 729230) in human. CC chemokine receptors (CCRs) are 7-transmembrane G protein-coupled receptors (GPCRs) that bind CC chemokines (CCLs) and mediate their biological activities. CCRs and CCLs play a central role in physiological and pathological recruitment of immune cells. CCR2 can interact with CCL2, CCL8 and CCL16, it is found on the surface of monocytes, activated memory T cells, B cells, and basophils in humans, and also in peritoneal macrophages in mice. CCR2 plays an important role in the recruitment of monocytes/macrophages and T cells. CCR2-knockout mice show a partial defect in macrophage function, decreased Th1 immune response, reduced delayed-type hypersensitivity reaction, and are more susceptible to pathogenic bacteria infections. Human CCR2 is a 7-transmembrane (a.a. 43-70, 81-100, 115-136, 154-178, 207-226, 244-268, 286-309) GPCR with an N-terminal extracellular domain (a.a. 1-42) and a C-terminoal cytoplasmic domain (a.a. 310-374).

Specyficzność

Clone 8C3.1 targets the N-terminal extracelluar domain of human CCR2 spliced isoforms 1&2 reported by Uniprot (P41597).

Immunogen

GST-tagged recombinant human N-terminal extracellular domain.

Zastosowanie

Flow Cytometry Analysis: 2 µL from a representative lot detected surface CCR2 expression among the gated lymphocytes population from one million human PMBCs.

Research Category
Inflammation & Immunology

This mouse monoclonal Anti-CCR2 Antibody, clone 8C3.1, Cat. No. MABF299 is validated for use in Flow Cytometry and Western Blotting for the detection of CCR2.

Jakość

Evaluated by Western Blotting in U937 cell lysate.

Western Blotting Analysis (WB): A 100,000 dilution of this antibody detected CCR2 in 10 µg of human lymphoma U937 monocyte lysate.

Opis wartości docelowych

~40-50 kDa observed. 41.92 kDa (isoform 1) and 41.06 kDa (isoform 2) calculated. Larger-than-calculated sizes and smeary banding pattern are consistent with the detection of differentially glycosylated CCR2 species. Uncharacterized bands may be observed in some lysate(s).

Postać fizyczna

Format: Purified

Protein G purified.

Purified mouse monoclonal antibody IgG2a in buffer containing 0.1 M Tris-Glycine (pH 7.4) 150 mM NaCl with 0.05% sodium azide

Przechowywanie i stabilność

Stable for 1 year at 2-8°C from date of receipt.

Inne uwagi

Concentration: Please refer to lot specific datasheet.

Oświadczenie o zrzeczeniu się odpowiedzialności

Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.

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Informacja o środkach bezpieczeństwa

• Kod klasy składowania: 12 - Non Combustible Liquids
• Klasa zagrożenia wodnego (WGK): WGK 1
• Temperatura zapłonu (°F): Not applicable
• Temperatura zapłonu (°C): Not applicable