MABE78

Anti-MiTF Antibody, clone 3D1.2

clone 3D1.2, from mouse

• Synonim(y): Microphthalmia-associated transcription factor, Class E basic helix-loop-helix protein 32, bHLHe32
• Kod UNSPSC: 12352203
• eCl@ss: 32160702
• NACRES: NA.41

Właściwości

• pochodzenie biologiczne: mouse
• Poziom jakości: 100
• forma przeciwciała: purified immunoglobulin
• rodzaj przeciwciała: primary antibodies
• klon: 3D1.2, monoclonal
• reaktywność gatunkowa: human, mouse
• metody: immunohistochemistry: suitable; western blot: suitable
• izotyp: IgMκ
• numer dostępu NCBI: NP_000239
• numer dostępu UniProt: O75030
• Warunki transportu: wet ice
• docelowa modyfikacja potranslacyjna: unmodified
• informacje o genach: human ... MITF(4286)

Opis

Opis ogólny

Microphthalmia-associated transcription factor (MiTF) is a basic helix loop helix leucine zipper (b HLH ZIP) transcription factor implicated in pigmentation, and development of mast cells and bone. Mutations in MiTF cause auditory pigmentary syndromes in humans, such as Waardenburg syndrome type II and type IIa, and Tietz syndrome. There are two known isoforms of MiTF, differing by 66 amino acids at the N-terminus. Shorter forms are expressed in melanocytes and run as two bands at 52 kDa and 56 kDa, whereas the longer Mi form runs as a cluster of bands at 60-70 kDa in osteoclasts and in B16 melonoma cells (but not other melanoma cell lines), as well as mast cells and heart. MiTF plays a critical role in the differentiation of various cell types as neural crest-derived melanocytes, mast cells, osteoclasts and optic cup-derived retinal pigment epithelium.

Immunogen

GST-tagged recombinant protein corresponding to human MiTF.

Zastosowanie

Anti-MiTF Antibody, clone 3D1.2 is an antibody against MiTF for use in Western Blotting & IHC.

Immunohistochemistry Analysis: A 1:100 dilution of this antibody detected MiTF in human melanoma tissue.

Research Category
Signaling

Research Sub Category
Transcription Factors

Jakość

Evaluated by Western Blot in mouse brain tissue lysate.

Western Blot Analysis: A 1:500 dilution of this antibody detected MiTF in 10 µg of mouse brain tissue lysate.

Opis wartości docelowych

~52 kDa observed. Multiple isoforms between 46-56 kDa are known to exist.

Powiązanie

Replaces: MAB3747

Postać fizyczna

Format: Purified

Purified mouse monoclonal IgMκ in buffer containing 0.01M PBS, 0.1% sodium azide, pH 7.1.

Przechowywanie i stabilność

Stable for 1 year at 2-8°C from date of receipt.

Komentarz do analizy

Control
Mouse brain tissue lysate

Oświadczenie o zrzeczeniu się odpowiedzialności

Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.

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Informacja o środkach bezpieczeństwa

• Kod klasy składowania: 12 - Non Combustible Liquids
• Klasa zagrożenia wodnego (WGK): WGK 1
• Temperatura zapłonu (°F): Not applicable
• Temperatura zapłonu (°C): Not applicable