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Key Documents

MABE446

Sigma-Aldrich

Anti-Histone H1° Antibody, clone 34

clone 34, from mouse

Synonim(y):

Histone H1.0, Histone H1(0), Histone H1°

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About This Item

Kod UNSPSC:
12352203
eCl@ss:
32160702
NACRES:
NA.41

pochodzenie biologiczne

mouse

Poziom jakości

forma przeciwciała

purified immunoglobulin

rodzaj przeciwciała

primary antibodies

klon

34, monoclonal

reaktywność gatunkowa

mouse, bovine, Xenopus, human, rat

reaktywność gatunkowa (przewidywana na podstawie homologii)

ox (immunogen homology)

metody

flow cytometry: suitable
immunocytochemistry: suitable
immunohistochemistry: suitable
western blot: suitable

izotyp

IgG1κ

numer dostępu NCBI

numer dostępu UniProt

Warunki transportu

wet ice

docelowa modyfikacja potranslacyjna

unmodified

informacje o genach

human ... H1F0(3005)

Opis ogólny

Histones are highly conserved proteins that serve as the structural scaffold for the organization of nuclear DNA into chromatin. The four core histones, H2A, H2B, H3, and H4, assemble into an octamer (2 molecules of each). Subsequently, 146 base pairs of DNA are wrapped around the octamer, forming a nucleosome. The linker histone, H1, interacts with linker DNA between nucleosomes and functions in the compaction of chromatin into 30nm chromatin fibers and higher order structures.

Specyficzność

This antibody recognizes Histone H1°.

Immunogen

Recombinant protein corresponding to Ox liver Histone H1°

Zastosowanie

Anti-Histone H1° Antibody, clone 34 is a highly specific mouse monoclonal antibody, that targets Histone H1 & has been tested in western blotting, ICC, IHC & Flow Cytometry.
Research Category
Epigenetics & Nuclear Function
Research Sub Category
Histones
Western Blotting Analysis: A representative lot from an independent laboratory detected Histone H1° in Xenopus embryo tissue lysate (Seigneurin, D., et al. (1995). Int J Dev Biol. 39(4):597-603.; Fu, G., et al. (2003). Biol Reprod. 68(5):1569-1576.).

Immunocytochemistry Analyis: A representative lot from an independent laboratory detected Histone H1° in Xenopus unfertilized eggs and early embryos (Fu, G., et al. (2003). Biol Reprod. 68(5):1569-1576.; Adenot, P. G., et al. (2000). J Cell Sci. 113(Pt 16):2897-2907.).

Immunohistochemistry Analysis: A representative lot from an independent laboratory detected Histone H1° in Xenopus embryo tissues (Grunwald, D., et al. (1995). Exp Cell Res. 218(2):586-595.).

Flow Cytometry Analyisis: A representative lot from an independent laboratory detected Histone H1° in FC (Grunwald, D., et al. (1999). Methods Mol Biol. 119:443-454.).

Jakość

Evaluated by Western Blotting in Jurkat cell lysate.

Western Blotting Analysis: 1 µg/mL of this antibody detected Histone H1° in 10 µg of Jurkat cell lysate.

Opis wartości docelowych

~30 kDa observed. Uncharacterized band(s) may be observed in some cell lysates.

Postać fizyczna

Format: Purified
Protein G Purified
Purified mouse monoclonal IgG1κ in buffer containing 0.1 M Tris-Glycine (pH 7.4), 150 mM NaCl with 0.05% sodium azide.

Przechowywanie i stabilność

Stable for 1 year at 2-8°C from date of receipt.

Komentarz do analizy

Control
Jurkat cell lysate

Inne uwagi

Concentration: Please refer to the Certificate of Analysis for the lot-specific concentration.

Oświadczenie o zrzeczeniu się odpowiedzialności

Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.
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Kod klasy składowania

12 - Non Combustible Liquids

Klasa zagrożenia wodnego (WGK)

WGK 1

Temperatura zapłonu (°F)

Not applicable

Temperatura zapłonu (°C)

Not applicable


Certyfikaty analizy (CoA)

Poszukaj Certyfikaty analizy (CoA), wpisując numer partii/serii produktów. Numery serii i partii można znaleźć na etykiecie produktu po słowach „seria” lub „partia”.

Masz już ten produkt?

Dokumenty związane z niedawno zakupionymi produktami zostały zamieszczone w Bibliotece dokumentów.

Odwiedź Bibliotekę dokumentów

Germaine Fu et al.
Biology of reproduction, 68(5), 1569-1576 (2003-02-28)
Oocytes and embryos of many species, including mammals, contain a unique linker (H1) histone, termed H1oo in mammals. It is uncertain, however, whether other H1 histones also contribute to the linker histone complement of these cells. Using immunofluorescence and radiolabeling
D Grunwald et al.
Experimental cell research, 218(2), 586-595 (1995-06-01)
It is known that a transition in the linker-histone variants takes place within chromatin during early development of Xenopus laevis; a cleavage-type H1 is replaced by the somatic type. Based on cytofluorimetric analysis of the distribution of the embryo cells
P G Adenot et al.
Journal of cell science, 113 ( Pt 16), 2897-2907 (2000-07-27)
A striking feature of early embryogenesis in a number of organisms is the use of embryonic linker histones or high mobility group proteins in place of somatic histone H1. The transition in chromatin composition towards somatic H1 appears to be
In situ analysis of chromatin proteins during development and cell differentiation using flow cytometry.
D Grunwald et al.
Methods in molecular biology (Clifton, N.J.), 119, 443-454 (2000-05-11)
Developmentally regulated chromatin acetylation and histone H1(0) accumulation.
Seigneurin, D, et al.
International Journal of Developmental Biology, 39, 597-603 (1995)

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