MABE320

Anti-c-ErbB2/c-Neu (Ab-5) Antibody, clone TA-1

clone TA-1, from mouse

• Synonim(y): Receptor tyrosine-protein kinase erbB-2, Metastatic lymph node gene 19 protein, MLN 19, Proto-oncogene Neu, Proto-oncogene c-ErbB-2, Tyrosine kinase-type cell surface receptor HER2, p185erbB2, CD340
• Kod UNSPSC: 12352203
• eCl@ss: 32160702
• NACRES: NA.41

Właściwości

• pochodzenie biologiczne: mouse
• Poziom jakości: 100
• forma przeciwciała: purified immunoglobulin
• rodzaj przeciwciała: primary antibodies
• klon: TA-1, monoclonal
• reaktywność gatunkowa: human
• metody: flow cytometry: suitable; immunofluorescence: suitable; immunoprecipitation (IP): suitable; western blot: suitable
• izotyp: IgG1κ
• numer dostępu NCBI: NP_001005862
• numer dostępu UniProt: P04626
• Warunki transportu: wet ice
• docelowa modyfikacja potranslacyjna: unmodified
• informacje o genach: human ... ERBB2(2064)

Opis

Opis ogólny

The ErbB2 (HER2, c-Neu, ErbB2, c-erbB2, or HER2/neu) protein is member of the human epidermal growth factor receptor (HER) family. It is a receptor tyrosine kinase containing an intracellular kinase domain and an extracellular ligand domain, although no endogenous ligand has yet been found. ErbB2 dimerizes with ErbB1, 3, and 4, and can activate different pathways including the PI3K, PLCγ, and MAPK pathways, depending on the ErbB receptor involved. Coupling with ErbB3 activates the PI3K pathway, whereas coupling with any of these three receptors can induce the MAPK pathway. ErbB2 signaling promotes cell proliferation and inhibits apoptosis. ErbB2 is overexpressed in various cancers and may play a role in the development and metastasis of gliomas, and ovarian, breast, lung, and gastric cancer.

Specyficzność

This antibody recognizes the extracellular domain of ErbB2/c-Neu.

Immunogen

Cells overexpressing human c-ErbB2/c-Neu

Epitope: Extracellular domain

Zastosowanie

Flow Cytometry Analysis: A representative lot from an independent laboratory detected c-ErbB2/c-Neu in HER-2 expressing SK-BR-3 cells (Montgomery, R. B., et al. (2005). Cancer Res. 65(2):650-656.).

Western Blot Analysis: A representative lot from an independent laboratory detected c-ErbB2/c-Neu in HER-2 expressing SK-BR-3 cells (Montgomery, R. B., et al. (2005). Cancer Res. 65(2):650-656.).

Immunoprecipitation Analysis: A representative lot from an independent laboratory precipitated c-ErbB2/c-Neu in IP (McKenzie, S. J., et al. (1989). 4(5):543-548.).

Immunofluorescence Analysis: A representative lot from an independent laboratory detected c-ErbB2/c-Neu in IF (McKenzie, S. J., et al. (1989). 4(5):543-548.).

Research Category
Epigenetics & Nuclear Function

Cell Signaling

Research Sub Category
Cell Cycle, DNA Replication & Repair

This Anti-c-ErbB2/c-Neu (Ab-5) Antibody, clone TA-1 is validated for use in Western Blotting, Flow Cytometry, Immunoprecipitation, Immunofluorescence for the detection of c-ErbB2/c-Neu (Ab-5).

Jakość

Evaluated by Flow Cytometry in SK-BR-3 cells.

Flow Cytometry Analysis: 0.5 µg of this antibody detected c-ErbB2/c-Neu in 0.5X10E6 SK-BR-3 cells.

Opis wartości docelowych

138 kDa calculated

Powiązanie

Replaces: 04-1127

Postać fizyczna

Format: Purified

Protein G Purified

Purified mouse monoclonal IgG1κ cultured supernatent in buffer containing 0.1 M Tris-Glycine (pH 7.4), 150 mM NaCl with 0.05% sodium azide.

Przechowywanie i stabilność

Stable for 1 year at 2-8°C from date of receipt.

Komentarz do analizy

Control
SK-BR-3 cells

Inne uwagi

Concentration: Please refer to the Certificate of Analysis for the lot-specific concentration.

Oświadczenie o zrzeczeniu się odpowiedzialności

Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.

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Informacja o środkach bezpieczeństwa

• Kod klasy składowania: 12 - Non Combustible Liquids
• Klasa zagrożenia wodnego (WGK): WGK 1
• Temperatura zapłonu (°F): Not applicable
• Temperatura zapłonu (°C): Not applicable