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MABC171

Sigma-Aldrich

Anti-Isocitrate Dehydrogenase 1 (IDH1)-R132H Antibody, clone HMab-1

clone HMab-1, from mouse

Synonim(y):

Isocitrate dehydrogenase [NADP] cytoplasmic, IDH, Cytosolic NADP-isocitrate dehydrogenase, IDP, NADP(+)-specific ICDH, Oxalosuccinate decarboxylase

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About This Item

Kod UNSPSC:
12352203
eCl@ss:
32160702
NACRES:
NA.41

pochodzenie biologiczne

mouse

Poziom jakości

100

forma przeciwciała

purified immunoglobulin

rodzaj przeciwciała

primary antibodies

klon

HMab-1, monoclonal

reaktywność gatunkowa

human

metody

immunocytochemistry: suitable
immunohistochemistry: suitable
western blot: suitable

izotyp

IgG1κ

numer dostępu NCBI

NP_005887

numer dostępu UniProt

O75874

Warunki transportu

wet ice

docelowa modyfikacja potranslacyjna

unmodified

informacje o genach

human ... IDH1(3417)

Opis ogólny

Isocitrate dehydrogenase 1 (IDH1) is a 46 kDa NADP-dependent enzyme, which catalyzes the decarboxylation of isocitrate into α-ketoglutarate. IDH1 may also play a role in the prevention of oxidative damage, and the shuttling of proteins to peroxisomes. It is widely reported that mutations in IDH1 result in various types of glioma.

Specyficzność

This antibody is specific for the R132H mutation of Isocitrate Dehydrogenase 1 (IDH1).

Immunogen

KLH-conjugated linear peptide corresponding to human Isocitrate Dehydrogenase 1 (IDH1).

Zastosowanie

Detect Isocitrate Dehydrogenase 1 (IDH1)-R132H using this Anti-Isocitrate Dehydrogenase 1 (IDH1)-R132H Antibody, clone HMab-1 validated for use in Western Blotting, IHC, ICC.
Research Category
Apoptosis & Cancer
Research Sub Category
Apoptosis - Additional
Western Blot Analysis: A representative lot of this antibody detected Isocitrate Dehydrogenase 1 (IDH1)-R132S in a panel of wild-type and mutated recombinant IDH1 proteins.

Immunohistochemistry Analysis: A representative lot from an independent laboratory detected Isocitrate Dehydrogenase 1 (IDH1)-R132S in anaplastic and diffuse astrocytoma tissues and in primary glioblastoma tissues (Kato, Y., et al. (2009). Biochem Biophys Res Commun. 390(3):547-51.).

Immunohistochemistry Analysis: A representative lot from an independent laboratory detected Isocitrate Dehydrogenase 1 (IDH1)-R132S in transfected glioblastoma and transfected LN229 cells (Kaneko, M. K., et al. (2011). Biochem Biophys Res Commun. 406(4):608-613.).

Jakość

Evaluated by Western Blot in MBP-tagged recombinant Isocitrate Dehydrogenase 1 (IDH1).

Western Blot Analysis: A 1:500 dilution of this antibody detected MBP tagged recombinant Isocitrate Dehydrogenase 1 (IDH1) in WB.

Opis wartości docelowych

~90 kDa observed. The calculated molecular weight of Isocitrate Dehydrogenase 1 (IDH1) is 47 kDa However, a Western Blot was performed using an MBP-tagged recombinant Isocitrate Dehydrogenase 1 (IDH1). Because MBP has a molecular weight of ~42 kDa, a band was observed at ~90 kDa

Postać fizyczna

Format: Purified
Protein G Purified
Purified mouse monoclonal IgG1κ in buffer containing 0.1 M Tris-Glycine (pH 7.4), 150 mM NaCl with 0.05% sodium azide.

Przechowywanie i stabilność

Stable for 1 year at 2-8°C from date of receipt.

Komentarz do analizy

Control
MBP tagged recombinant Isocitrate Dehydrogenase 1 (IDH1)

Oświadczenie o zrzeczeniu się odpowiedzialności

Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.
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Kod klasy składowania

12 - Non Combustible Liquids

Klasa zagrożenia wodnego (WGK)

WGK 1

Temperatura zapłonu (°F)

Not applicable

Temperatura zapłonu (°C)

Not applicable

Certyfikaty analizy (CoA)

Poszukaj Certyfikaty analizy (CoA), wpisując numer partii/serii produktów. Numery serii i partii można znaleźć na etykiecie produktu po słowach „seria” lub „partia”.

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Dokumenty związane z niedawno zakupionymi produktami zostały zamieszczone w Bibliotece dokumentów.

Odwiedź Bibliotekę dokumentów

A monoclonal antibody IMab-1 specifically recognizes IDH1R132H, the most common glioma-derived mutation.
Kato, Yukinari, et al.
Biochemical and biophysical research communications, 390, 547-551 (2009)
Intraoperative mass spectrometry mapping of an onco-metabolite to guide brain tumor surgery.
Santagata, S; Eberlin, LS; Norton, I; Calligaris, D; Feldman, DR; Ide, JL; Liu, X; Wiley et al.
Proceedings of the National Academy of Sciences of the USA null
Roberta Soares Faccion et al.
Cellular oncology (Dordrecht), 41(2), 141-157 (2018-01-28)
Diffuse astrocytic tumors are the most frequently occurring primary central nervous system (CNS) tumors. Their histological sub-classification into diffuse astrocytoma (DA), anaplastic astrocytoma (AA) and glioblastoma (GB) is challenging and the available prognostic factors are limited to age and tumor
Establishment of a novel monoclonal antibody SMab-1 specific for IDH1-R132S mutation.
Kaneko, Mika Kato, et al.
Biochemical and biophysical research communications, 406, 608-613 (2011)
Irati Hervás-Corpión et al.
Cancers, 13(21) (2021-11-14)
Glioblastoma (GB) is the most aggressive form of glioma and is characterized by poor prognosis and high recurrence despite intensive clinical interventions. To retrieve the key factors underlying the high malignancy of GB with potential diagnosis utility, we combined the

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