MABC1122

Anti-PD-1 Antibody, clone EH33

clone EH33.1F9.G4.5, from mouse

• Synonim(y): Programmed cell death protein 1, hPD-1, CD279
• Kod UNSPSC: 12352203
• eCl@ss: 32160702

Właściwości

• pochodzenie biologiczne: mouse
• Poziom jakości: 100
• forma przeciwciała: purified immunoglobulin
• rodzaj przeciwciała: primary antibodies
• klon: EH33.1F9.G4.5, monoclonal
• reaktywność gatunkowa: human
• opakowanie: antibody small pack of 25 μg
• metody: immunohistochemistry: suitable (paraffin)
• izotyp: IgG2aκ
• numer dostępu NCBI: NP_005009.2
• numer dostępu UniProt: Q15116
• docelowa modyfikacja potranslacyjna: unmodified
• informacje o genach: human ... PDCD1(5133)

Opis

Opis ogólny

Programmed cell death protein 1 (UniProt: Q15116; also known as Protein PD-1, hPD-1, CD279) is encoded by the PDCD1 (also known as PD1) gene (Gene ID: 5133) in human. PD-1 is a monomeric inhibitory cell surface receptor involved in the regulation of T-cell function during immunity and tolerance. PD-1 is synthesized with a signal peptide (aa 1-20), which is subsequently cleaved off. The mature form contains an extracellular domain (aa 24-170), a transmembrane domain (aa 171-191), and a cytoplasmic domain (aa 192-288). PD-1 also contains a single N-terminal immunoglobulin variable region (IgV) like domain. PD-1 and its ligands, PD-L1 and PD-L2 play a key role in the maintenance of peripheral tolerance, a process by which the quiescence of autoreactive mature T cells is maintained. However, tumors and pathogens that cause chronic infections can exploit this pathway to escape T-cell mediated tumor-specific and pathogen-specific immunity. The effector functions of T-cells expressing PD-1 can be downregulated by PD-L1 or PD-L2 expressed by the tumor cells. PD-1 lacks SH2- or SH3-binding motifs on its cytoplasmic tail, but contains the N-terminal sequence VDYGEL that forms an immunoreceptor tyrosine-based inhibition motif (V/I/LxYxxL), which recruits SH2 domain-containing phosphatases. The cytoplasmic tail also contains the C-terminal sequence TEYATI, which forms an immunoreceptor tyrosine-based switch motif (TxYxxL). PD-1 ligation is reported to inhibit the activation of T-cell receptor proximal kinases, which results in attenuation of Lck-mediated phosphorylation of ZAP-70 and initiation of downstream events. PD-1 is also reported to impair the activation of the MEK-ERK MAP kinase pathway by inhibiting activation of PLC- 1 and Ras. (Ref.: Boussiotis, VA (2016). N. Engl. J. Med. 375; 1767-1778).

Specyficzność

Clone EH33 specifically detects PD-1 in human cells. It targets an epitope with the extracellular domain.

Immunogen

Epitope: extracellular domain

Recombinant protein fragment corresponding to the extracellular domain of human PD-1 (as an Ig fusion protein).

Zastosowanie

Anti-PD-1, clone EH33, Cat. No. MABC1122, is a mouse monoclonal antibody that detects Programmed cell death protein 1 (PD-1) in human cells and has been tested for use in Immunohistochemistry (Paraffin).

Immunohistochemistry Analysis: A representative lot detected PD-1 in Immunohistochemistry applications (Sridharan, V., et. al. (2016). Cancer Immunol Res. 4(8):679-87; Calles, A., et. al. (2015). J Thorac Oncol. 10(12):1726-35; Bachireddy, P., et. al. (2014). Blood. 123(9):1412-21).

Research Category
Inflammation & Immunology

Jakość

Evaluated by Immunohistochemistry in human tonsil and human bone marrow tissues.

Immunohistochemistry Analysis: A 1:250 dilution of this antibody detected PD-1 in human tonsil and human bone marrow tissues.

Opis wartości docelowych

31.65 kDa calculated.

Postać fizyczna

Format: Purified

Protein G purified

Purified mouse monoclonal antibody IgG2a in buffer containing 0.1 M Tris-Glycine (pH 7.4), 150 mM NaCl with 0.05% sodium azide.

Przechowywanie i stabilność

Stable for 1 year at 2-8°C from date of receipt.

Inne uwagi

Concentration: Please refer to lot specific datasheet.

Oświadczenie o zrzeczeniu się odpowiedzialności

Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.

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Informacja o środkach bezpieczeństwa

• Kod klasy składowania: 12 - Non Combustible Liquids
• Klasa zagrożenia wodnego (WGK): WGK 1
• Temperatura zapłonu (°F): Not applicable
• Temperatura zapłonu (°C): Not applicable