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Key Documents

MAB5350

Sigma-Aldrich

Anti-Choline Acetyltransferase Antibody, clone 28C4

clone 28C4, Chemicon®, from mouse

Synonim(y):

ChAT

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About This Item

Kod UNSPSC:
12352203
eCl@ss:
32160702
NACRES:
NA.41

pochodzenie biologiczne

mouse

Poziom jakości

forma przeciwciała

purified immunoglobulin

rodzaj przeciwciała

primary antibodies

klon

28C4, monoclonal

reaktywność gatunkowa

human, rat, guinea pig, monkey

producent / nazwa handlowa

Chemicon®

metody

ELISA: suitable
immunohistochemistry: suitable
western blot: suitable

izotyp

IgG

numer dostępu UniProt

Warunki transportu

wet ice

docelowa modyfikacja potranslacyjna

unmodified

informacje o genach

human ... CHAT(1103)
rat ... Chat(290567)
rhesus monkey ... Chat(709977)

Specyficzność

Reacts with Choline Acetyltransferase (ChAT).

Immunogen

Purified human placenta ChAT.

Zastosowanie

Anti-Choline Acetyltransferase Antibody, clone 28C4 detects level of Choline Acetyltransferase & has been published & validated for use in ELISA, IH & WB.
Research Category
Neuroscience
Research Sub Category
Neurotransmitters & Receptors

Neuronal & Glial Markers
Western blotting: 1:1,000-1:10,000 using a high sensitivity ECL kit. Recognizes a protein of ~67 kDa. May also recognize a protein of 35 kDa which is thought to be a degradation product. Suggested dilution buffer is TBS containing 5% skim milk powder and 0.05% Tween 20.

Immunohistochemistry on free-floating frozen sections of Rhesus monkey brain fixed with 4% paraformaldehyde and coronal vibratome sections of rat brain fixed with 4% paraformaldehyde and 0.2% glutaraldehyde.

ELISA (two side)

Optimal working dilutions must be determined by end user.

Postać fizyczna

Format: Purified
Purified immunoglobulin. Liquid.

Przechowywanie i stabilność

Maintain at 2-8°C in undiluted aliquots for up to 6 months after date of receipt.

Inne uwagi

Concentration: Please refer to the Certificate of Analysis for the lot-specific concentration.

Informacje prawne

CHEMICON is a registered trademark of Merck KGaA, Darmstadt, Germany

Oświadczenie o zrzeczeniu się odpowiedzialności

Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.
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Kod klasy składowania

10 - Combustible liquids

Klasa zagrożenia wodnego (WGK)

WGK 2

Temperatura zapłonu (°F)

Not applicable

Temperatura zapłonu (°C)

Not applicable


Certyfikaty analizy (CoA)

Poszukaj Certyfikaty analizy (CoA), wpisując numer partii/serii produktów. Numery serii i partii można znaleźć na etykiecie produktu po słowach „seria” lub „partia”.

Masz już ten produkt?

Dokumenty związane z niedawno zakupionymi produktami zostały zamieszczone w Bibliotece dokumentów.

Odwiedź Bibliotekę dokumentów

Zhaoqin Huang et al.
Molecular medicine reports, 11(5), 3285-3290 (2015-01-17)
The present study was conducted to investigate the 11C‑Choline metabolic mechanism and examine the association between 11C‑Choline metabolism and uptake in different pathological types of lung cancer. A total of 18 tumor specimens and corresponding normal lung tissues were collected
Katarina Zvarova et al.
The Journal of comparative neurology, 489(4), 501-517 (2005-07-19)
We examined the distribution and fate of cocaine- and amphetamine-regulated transcript peptide (CARTp)(55-102)-immunoreactive (IR) structures in the neonatal and adult rat urinary bladder. Double-labeling studies examining CARTp with tyrosine hydroxylase (TH), neuronal nitric oxide synthase (nNOS), or choline acetyltransferase (ChAT)
ELISA methods to measure cholinergic markers and nerve growth factor receptors in cortex, hippocampus, prefrontal cortex, and basal forebrain from rat brain.
Gearhart, Debra A, et al.
Journal of Neuroscience Methods, 150, 159-173 (2006)
Hanqing Tang et al.
International heart journal, 62(3), 647-657 (2021-05-18)
Bioinformatics analysis showed that miR-448-5p expression in the myocardial tissue of rats with myocardial infarction significantly increased, suggesting that it may participate in myocardial cell apoptosis in myocardial infarction. This study aimed to explore the protective effects of miR-448-5p on
Ji-Woon Kim et al.
PloS one, 9(8), e104927-e104927 (2014-08-19)
Autism spectrum disorder (ASD) is a group of pervasive developmental disorders with core symptoms such as sociability deficit, language impairment, and repetitive/restricted behaviors. Although worldwide prevalence of ASD has been increased continuously, therapeutic agents to ameliorate the core symptoms especially

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