MAB5270
Anti-Choline Acetyltransferase Antibody, clone 1.B3.9B3
clone 1.B3.9B3, Chemicon®, from mouse
Synonim(y):
ChAT
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About This Item
Kod UNSPSC:
12352203
eCl@ss:
32160702
NACRES:
NA.41
Polecane produkty
pochodzenie biologiczne
mouse
Poziom jakości
forma przeciwciała
purified immunoglobulin
rodzaj przeciwciała
primary antibodies
klon
1.B3.9B3, monoclonal
reaktywność gatunkowa
human, rat, pig
producent / nazwa handlowa
Chemicon®
metody
ELISA: suitable
immunohistochemistry (formalin-fixed, paraffin-embedded sections): suitable
western blot: suitable
izotyp
IgG1
numer dostępu NCBI
numer dostępu UniProt
Warunki transportu
wet ice
docelowa modyfikacja potranslacyjna
unmodified
informacje o genach
human ... CHAT(1103)
pig ... Chat(396896)
rat ... Chat(290567)
Specyficzność
Reacts with choline acetyltransferase (ChAT)-positive cells from humans, rats and pigs (Ostermann-Fatif et al., 1992a,b).
Immunogen
Highly purified ChAT obtained from porcine brain (Ostermann-Fatif et al., 1990).
Zastosowanie
Research Category
Neuroscience
Neuroscience
Research Sub Category
Neurotransmitters & Receptors
Neurotransmitters & Receptors
This Anti-Choline Acetyltransferase Antibody, clone 1.B3.9B3 is validated for use in ELISA, IH, IH(P), WB for the detection of Choline Acetyltransferase.
Western blot
ELISA
Immunohistochemistry: 10-20 μg/mL in paraffin embedded sections {See Ratcliffe et al. 1998}
Optimal working dilutions and protocols must be determined by end user.
ELISA
Immunohistochemistry: 10-20 μg/mL in paraffin embedded sections {See Ratcliffe et al. 1998}
Optimal working dilutions and protocols must be determined by end user.
Opis wartości docelowych
69 kDa
Postać fizyczna
Format: Purified
Protein A purified
Purified immunoglobulin. Lyophilized from 0.02 M Phosphate buffer, 0.25 M NaCl with 0.1% sodium azide. Reconstitute to 100 μg/mL with sterile distilled water.
Przechowywanie i stabilność
Maintain lyophilized antibody at 2–8°C in undiluted aliquots for up to 6 months. Maintain reconstituted antibody at -20°C in undiluted aliquots for up to 6 months.
Komentarz do analizy
Control
Brain tissue
Brain tissue
Inne uwagi
Concentration: Please refer to the Certificate of Analysis for the lot-specific concentration.
Informacje prawne
CHEMICON is a registered trademark of Merck KGaA, Darmstadt, Germany
Oświadczenie o zrzeczeniu się odpowiedzialności
Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.
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Masz już ten produkt?
Dokumenty związane z niedawno zakupionymi produktami zostały zamieszczone w Bibliotece dokumentów.
Angela Marina Montalbano et al.
Mediators of inflammation, 2016, 9063842-9063842 (2016-06-15)
IL-17A is overexpressed in the lung during acute neutrophilic inflammation. Acetylcholine (ACh) increases IL-8 and Muc5AC production in airway epithelial cells. We aimed to characterize the involvement of nonneuronal components of cholinergic system on IL-8 and Muc5AC production in bronchial
Production of specific antisera and monoclonal antibodies to choline acetyltransferase: characterization and use for identification of cholinergic neurons.
Eckenstein, F and Thoenen, H
The Embo Journal, 1, 363-368 (1982)
R E Watson et al.
Peptides, 7(1), 155-159 (1986-01-01)
Use of an ethylene glycol based cryoprotectant solution has been found to be effective for the long-term storage of brain tissue either in block form or as freely floating sections prior to immunocytochemical processing. Storage of tissue in the solution
C Ostermann-Latif et al.
Journal of immunological methods, 157(1-2), 73-79 (1993-01-04)
A specific, sensitive, and reliable sandwich-ELISA (enzyme-linked immunosorbent assay) has been established for the determination of choline acetyltransferase (CHAT) from porcine brain. The detection limit of the assay was 30 micrograms/l and the assay was linear up to 300 micrograms/l.
Large-scale purification of choline acetyltransferase and production of highly specific antisera.
Ostermann, C, et al.
European Journal of Biochemistry, 192, 215-218 (1990)
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