MAB351
Anti-Glutamate Decarboxylase Antibody, 65 kDa isoform, clone GAD-6
clone GAD-6, Chemicon®, from mouse
Synonim(y):
GAD65
Zaloguj sięWyświetlanie cen organizacyjnych i kontraktowych
About This Item
Kod UNSPSC:
12352203
eCl@ss:
32160702
NACRES:
NA.41
Polecane produkty
pochodzenie biologiczne
mouse
Poziom jakości
forma przeciwciała
purified immunoglobulin
rodzaj przeciwciała
primary antibodies
klon
GAD-6, monoclonal
reaktywność gatunkowa
rat, human
producent / nazwa handlowa
Chemicon®
metody
immunohistochemistry: suitable
western blot: suitable
izotyp
IgG2a
numer dostępu NCBI
numer dostępu UniProt
Warunki transportu
dry ice
docelowa modyfikacja potranslacyjna
unmodified
informacje o genach
human ... GAD2(2572)
Opis ogólny
Gutamic acid decarboxylase (GAD; E.C. 4.1.1.15) is the enzyme responsible for the conversion of glutamic acid to gamma-aminobutyric acid (GABA), the major inhibitory transmitter in higher brain regions, and putative paracrine hormone in pancreatic islets. Two molecular forms of GAD (65 kDa and 67 kDa, 64% aa identity between forms) are highly conserved and both forms are expressed in the CNS, pancreatic islet cells, testis, oviduct and ovary. The isoforms are regionally distributed cytoplasmically in the brains of rats and mice (Sheikh, S. et al. 1999). GAD65 is an ampiphilic, membrane-anchored protein (585 a.a.), encoded on human chromosome 10, and is responsible for vesicular GABA production. GAD67 is cytoplasmic (594 a.a.), encoded on chromosome 2, and seems to be responsible for significant cytoplasmic GABA production. GAD expression changes during neural development in rat spinal cord. GAD65 is expressed transiently in commissural axons around E13 but is down regulated the next day while GAD67 expression increases mostly in the somata of those neurons (Phelps, P. et al. 1999). In mature rat pancreas, GAD65 and GAD67 appear to be differentially localized, GAD65 primarily in insulin-containing beta cells and GAD67 in glucagon-containing (A) cells (Li, L. et al. 1995). GAD67 expression seems to be particularly plastic and can change in response to experimental manipulation (for example neuronal stimulation or transection) or disease progression and emergent disorders like schizophrenia (Volk et al., 2000). Colocalization of the two GAD isoforms also shows changes in GAD65/GAD67 distributions correlated with certain disease states such as IDDM and SMS.
Specyficzność
Recognizes the lower molecular weight isoform of the two GAD isoforms identified in brain (Gottlieb, et al., 1986; Chang & Gottlieb, 1988). This monclonal antibody can be used for immunohistochemical localization in brain or pancreas. Anti-GAD has also been used to label purified GAD on Western blots (Chang & Gottlieb, 1988).
Immunogen
Purified rat brain glutamic acid decarboxylase.
Zastosowanie
Anti-GAD Antibody. 65 kDa isoform, clone GAD-6 detects level of Glutamate Decarboxylase & has been published & validated for use in IH & WB.
Immunohistochemistry: ( 1 μg/mL * See protocol)
Western blot
Optimal working dilutions must be determined by end user.
APPLICATION NOTES FOR MAB351
IMMUNOHISTOCHEMISTRY
1) Perfuse rats with 100 mM phosphate buffer, pH 7.4 containing 1% paraformaldehyde, 0.34% L-lysine and 0.05% m-periodate (1% PLP).
2) Postfix brains in 1% PLP for 1-2 hours.
3) Transfer brains to 100 mM phosphate buffer containing 30% sucrose and gently agitate on a shaker platform at +4°C for 48-60 hours.
4) Using a sliding microtome, cut 30 mm sections of frozen cerebellum. As the sections are cut, collect them in a vial of cold 100 mM phosphate buffer.
5) Incubate sections in PBS containing 1.5% normal serum and 0.2% Triton X-100 for 30 minutes.
6) On a shaker platform, incubate sections with MAB351 (diluted 1 μg/mL in PBS containing 1.5% normal serum and 0.2% Triton X-100) for 12-36 hours at +4°C.
7) On a shaker platform, rinse sections eight times, 10-15 minutes per rinse, in PBS.
8) Detect with standard secondary antibody detection system (PAP, ABC, etc.).
9) Mount sections, dehydrate, and apply coverslips.
Western blot
Optimal working dilutions must be determined by end user.
APPLICATION NOTES FOR MAB351
IMMUNOHISTOCHEMISTRY
1) Perfuse rats with 100 mM phosphate buffer, pH 7.4 containing 1% paraformaldehyde, 0.34% L-lysine and 0.05% m-periodate (1% PLP).
2) Postfix brains in 1% PLP for 1-2 hours.
3) Transfer brains to 100 mM phosphate buffer containing 30% sucrose and gently agitate on a shaker platform at +4°C for 48-60 hours.
4) Using a sliding microtome, cut 30 mm sections of frozen cerebellum. As the sections are cut, collect them in a vial of cold 100 mM phosphate buffer.
5) Incubate sections in PBS containing 1.5% normal serum and 0.2% Triton X-100 for 30 minutes.
6) On a shaker platform, incubate sections with MAB351 (diluted 1 μg/mL in PBS containing 1.5% normal serum and 0.2% Triton X-100) for 12-36 hours at +4°C.
7) On a shaker platform, rinse sections eight times, 10-15 minutes per rinse, in PBS.
8) Detect with standard secondary antibody detection system (PAP, ABC, etc.).
9) Mount sections, dehydrate, and apply coverslips.
Research Category
Neuroscience
Neuroscience
Research Sub Category
Neurotransmitters & Receptors
Neurotransmitters & Receptors
Opis wartości docelowych
65 kDa
Postać fizyczna
Format: Purified
Protein A purified
Purified immunoglobulin. Lyophilized from 10 mM potassium phosphate, 70 mM sodium chloride, pH 7.4, 0.02% sodium azide. Reconstitute with 100 μL of sterile water (1 mg/mL).
Przechowywanie i stabilność
Maintain lyophilized material at -20°C for up to 12 months. After reconstitution maintain frozen at -20°C in undiluted aliquots for up to 6 months. Avoid repeated freeze/thaw cycles.
Komentarz do analizy
Control
Rat brain tissue
human brain lysate
Rat brain tissue
human brain lysate
Inne uwagi
Concentration: Please refer to the Certificate of Analysis for the lot-specific concentration.
Informacje prawne
CHEMICON is a registered trademark of Merck KGaA, Darmstadt, Germany
Oświadczenie o zrzeczeniu się odpowiedzialności
Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.
This page may contain text that has been machine translated.
Nie możesz znaleźć właściwego produktu?
Wypróbuj nasz Narzędzie selektora produktów.
polecane
Hasło ostrzegawcze
Warning
Zwroty wskazujące rodzaj zagrożenia
Zwroty wskazujące środki ostrożności
Klasyfikacja zagrożeń
Acute Tox. 4 Dermal - Acute Tox. 4 Inhalation - Acute Tox. 4 Oral - Aquatic Chronic 3
Kod klasy składowania
13 - Non Combustible Solids
Klasa zagrożenia wodnego (WGK)
WGK 3
Temperatura zapłonu (°F)
Not applicable
Temperatura zapłonu (°C)
Not applicable
Certyfikaty analizy (CoA)
Poszukaj Certyfikaty analizy (CoA), wpisując numer partii/serii produktów. Numery serii i partii można znaleźć na etykiecie produktu po słowach „seria” lub „partia”.
Masz już ten produkt?
Dokumenty związane z niedawno zakupionymi produktami zostały zamieszczone w Bibliotece dokumentów.
Differential activation of neuronal cell types in the basolateral amygdala by corticotropin releasing factor.
Rostkowski, AB; Leitermann, RJ; Urban, JH
Neuropeptides null
Synaptic reorganisation of the medial amygdala during puberty.
Cooke BM
Journal of Neuroendocrinology null
Thalamocortical dysfunction and thalamic injury after asphyxial cardiac arrest in developing rats.
Shoykhet, M; Simons, DJ; Alexander, H; Hosler, C; Kochanek, PM; Clark, RS
The Journal of Neuroscience null
Quantitative effects produced by modifications of neuronal activity on the size of GABAA receptor clusters in hippocampal slice cultures.
Serge Marty, Rosine Wehrle, Jean-Marc Fritschy, Constantino Sotelo
The European Journal of Neuroscience null
Quantitative analysis of ER alpha and GAD colocalization in the hippocampus of the adult female rat.
S A Hart, J D Patton, C S Woolley, S A Hart, J D Patton, C S Woolley
The Journal of Comparative Neurology null
Nasz zespół naukowców ma doświadczenie we wszystkich obszarach badań, w tym w naukach przyrodniczych, materiałoznawstwie, syntezie chemicznej, chromatografii, analityce i wielu innych dziedzinach.
Skontaktuj się z zespołem ds. pomocy technicznej