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Key Documents

MAB2058Z

Sigma-Aldrich

Anti-Integrin β4 Antibody, clone ASC-3, azide free

clone ASC-3, Chemicon®, from mouse

Synonim(y):

CD104, MAB2058

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About This Item

Kod UNSPSC:
12352203
eCl@ss:
32160702
NACRES:
NA.41

pochodzenie biologiczne

mouse

Poziom jakości

100

forma przeciwciała

purified immunoglobulin

rodzaj przeciwciała

primary antibodies

klon

ASC-3, monoclonal

reaktywność gatunkowa

human

producent / nazwa handlowa

Chemicon®

metody

flow cytometry: suitable
immunohistochemistry: suitable
immunoprecipitation (IP): suitable

izotyp

IgG1κ

numer dostępu NCBI

NM_000213.3
NM_001005619.1
NM_001005731.1

numer dostępu UniProt

P16144

Warunki transportu

dry ice

docelowa modyfikacja potranslacyjna

unmodified

informacje o genach

human ... ITGB4(3691)

Powiązane kategorie

Specyficzność

Reacts with Human beta4 integrin (CD104).

Immunogen

Human squamous cell carcinoma cells (SCC9)

Zastosowanie

Anti-Integrin β4 Antibody, clone ASC-3, azide free detects level of Integrin β4 & has been published & validated for use in FC, IH & IP.
Inhibition of cell attachment: inhibits SCC9 cell adhesion to human laminin and laminin GD-2 peptide, but not to EHS laminin, type IV collagen or fibronectin. Does not inhibit SKOV-3 cell adhesion to laminin, fibronectin or type IV collagen. Immunohistochemistry: on acetone-fixed frozen human epithelial tissues including tongue, foreskin and ovary. Not for use on formalin-fixed tissue. Flow cytometry: stains human squamous cell carcinoma (SCC9), ovarian carcinoma (SKOV-3) and umbilical vein endothelium (HUVEC). Stains RKO or K562 cells transfected for CD104 expression. Does not stain monocytes, neutrophils, lymphocytes, T cells, B cells or platelets. Immunoprecipitation: precipitates PAGE bands of 66, 76, 120, 135 and 200kDa (reduced) or 66, 110, 125, 130 and 190kDa (non-reduced) from surface-biotinylated SCC9 cells. Working dilutions must be determined by end user.
Research Category
Cell Structure
Research Sub Category
Integrins

Postać fizyczna

Format: Purified
Protein A Purified mouse immunoglobulin in 20 mM sodium phosphate, 250 mM NaCl, pH. 7.6, containing no preservatives.
Protein A purified

Przechowywanie i stabilność

Maintain for 1 year at -20°C from date of shipment. Aliquot to avoid repeated freezing and thawing. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.

Komentarz do analizy

Control
A431 cell lysate

Inne uwagi

Concentration: Please refer to the Certificate of Analysis for the lot-specific concentration.

Informacje prawne

CHEMICON is a registered trademark of Merck KGaA, Darmstadt, Germany

Oświadczenie o zrzeczeniu się odpowiedzialności

Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.
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Kod klasy składowania

12 - Non Combustible Liquids

Klasa zagrożenia wodnego (WGK)

WGK 2

Temperatura zapłonu (°F)

Not applicable

Temperatura zapłonu (°C)

Not applicable

Certyfikaty analizy (CoA)

Poszukaj Certyfikaty analizy (CoA), wpisując numer partii/serii produktów. Numery serii i partii można znaleźć na etykiecie produktu po słowach „seria” lub „partia”.

Masz już ten produkt?

Dokumenty związane z niedawno zakupionymi produktami zostały zamieszczone w Bibliotece dokumentów.

Odwiedź Bibliotekę dokumentów

M Schmelz et al.
The Prostate, 48(2), 79-92 (2001-07-04)
The generation of prostatic cell lines provides in vitro models for experimental studies of the pathogenesis of prostate carcinoma. Therefore, we established and characterized a new human prostate epithelial cell line, PEAZ-1 (prostate epithelial Arizona-1). The PEAZ-1 cells were grown
Michael Y Lin et al.
Annals of neurology, 72(1), 112-123 (2012-07-26)
Chronic nerve compression (CNC) injuries occur when peripheral nerves are subjected to sustained mechanical forces, with increasing evidence implicating Schwann cells as key mediators. Integrins, a family of transmembrane adhesion molecules that are capable of intracellular signaling, have been implicated
Tobias Wiedemann et al.
Gut, 61(7), 986-996 (2012-01-31)
One of the most important hormones in the human stomach is the peptide gastrin. It is mainly required for the regulation of gastric pH but is also involved in growth and differentiation of gastric epithelial cells. In Helicobacter pylori infected
A new DPB1 allele (DPB1*5901) identified by reverse dot blot hybridization and confirmed by DNA sequencing.
Perrier, P and Dormoy, A
Tissue Antigens, 47, 356-359 (1996)

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