MAB1904

Anti-Laminin-1 A&B chains Antibody, cross region, clone AL-2

clone AL-2, Chemicon^®, from rat

• Synonim(y): laminin alpha1, laminin beta1
• Kod UNSPSC: 12352203
• eCl@ss: 32160702
• NACRES: NA.41

Właściwości

• pochodzenie biologiczne: rat
• Poziom jakości: 100
• forma przeciwciała: purified antibody
• rodzaj przeciwciała: primary antibodies
• klon: AL-2, monoclonal
• reaktywność gatunkowa: mouse
• producent / nazwa handlowa: Chemicon^®
• metody: ELISA: suitable; immunofluorescence: suitable; immunohistochemistry: suitable (paraffin); immunoprecipitation (IP): suitable; western blot: suitable
• izotyp: IgG1κ
• numer dostępu NCBI: NM_005559.2
• numer dostępu UniProt: P25391; P55268
• Warunki transportu: wet ice
• docelowa modyfikacja potranslacyjna: unmodified
• informacje o genach: mouse ... Lama1(16772)

Opis

Opis ogólny

Laminins are secreted glycoproteins incorporated into cell-associated extracellular matrices and an integral part of the structural scaffolding in most animal tissues. The laminin protein is made up of three polypeptide chains; α, β and γ, accounting for its flexibility in connecting various molecules. When originally characterized, laminin-1 clone AL-2 reacted with Engelbreth-Holm-Swarm (EHS) mouse laminin A & B chains on the long arm near the cross region, (Skubitz, 1987). More recently, investigations, (Schèele, 2006) have shown that the clone binds laminin β1 under reducing conditions as well as native ELISAs and that the affinity is increased by the presence of the laminin α1 chain. Thus there is significant laminin β1 reactivity with clone AL-2.

Specyficzność

Other species have not been tested.

Reacts with EHS mouse laminin A & B chains on the long arm near the cross region.

Immunogen

Epitope: Near cross region

Laminin isolated from EHS mouse sarcoma.

Zastosowanie

Detect Laminin-1 A&B chains using this Anti-Laminin-1 A&B chains Antibody, cross region, clone AL-2 validated for use in ELISA, IF, IH(P), IP & WB.

Immunofluorescence:
An independent laboratory (Schèele, S., 2006) has verified anti-laminin clone AL-2 to work using immunofluorescence techniques on mouse muscle tissue, including both transgenic and wild-type tissues.

Research Category
Cell Structure

Research Sub Category
ECM Proteins

Jakość

Evaluated on a representative lot by Western blot on purified mouse laminin-1.

Opis wartości docelowych

~200 kDa

Postać fizyczna

Format: Purified

Protein G Purified

Purified in 0.1M Tris-Glycine (pH 7.4) 150mM NaCl with 0.05% NaN3.

Przechowywanie i stabilność

Maintain at 2-8°C in undiluted aliquots for up to 1 year after date of receipt.

Inne uwagi

Concentration: Please refer to the Certificate of Analysis for the lot-specific concentration.

Informacje prawne

CHEMICON is a registered trademark of Merck KGaA, Darmstadt, Germany

Oświadczenie o zrzeczeniu się odpowiedzialności

Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.

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Informacja o środkach bezpieczeństwa

• Kod klasy składowania: 12 - Non Combustible Liquids
• Klasa zagrożenia wodnego (WGK): WGK 1
• Temperatura zapłonu (°F): Not applicable
• Temperatura zapłonu (°C): Not applicable