About This Item
Kod UNSPSC:
12352200
eCl@ss:
32160405
NACRES:
NA.77
Polecane produkty
Opis ogólny
Antipain is a serine and cysteine reversible protease inhibitor. It inhibits papain and trypsin. Plasmin is inhibited to a small extent as well. More specific than leupeptin for papain and trypsin.
Sequence:
[(S)-1-Carboxy-2-Phenylethyl]-Carbamoyl-L-Arg-L-Val-Argininal-HCL-2H2O.
M.W: 604.71
Working solutions: 1-100μM.
Specific Activity: Varies with lot, IC50 is approximately 0.20μg/mL (papain/casein, pH 7.4 37°C).
Stable for several hours. Stock solutions of 10mM in water or buffer are stable for 1 week at 4ºC, 1 month at -20ºC. Also soluable in MeOH or DMSO.
5mg/827 microliters of water = 10mM solution. Dilute 1:100 for a 100μM solution.
Source: Microbial origin. Not synthetically made.
Sequence:
[(S)-1-Carboxy-2-Phenylethyl]-Carbamoyl-L-Arg-L-Val-Argininal-HCL-2H2O.
M.W: 604.71
Working solutions: 1-100μM.
Specific Activity: Varies with lot, IC50 is approximately 0.20μg/mL (papain/casein, pH 7.4 37°C).
Stable for several hours. Stock solutions of 10mM in water or buffer are stable for 1 week at 4ºC, 1 month at -20ºC. Also soluable in MeOH or DMSO.
5mg/827 microliters of water = 10mM solution. Dilute 1:100 for a 100μM solution.
Source: Microbial origin. Not synthetically made.
Zastosowanie
Proteinase inhibitor; Inhibits papain and trypsin. Plasmin is inhibited to a small extent.
Działania biochem./fizjol.
Inhibitor Type: Protease Inhibitors
Jakość
pH 1% aqueous solution: 5.2
Postać fizyczna
Lyophilized no preservatives.
Properties: Soluble in H2O, methanol and DMSO (Stock solution: 10 mM). Stable at -20° C.
Mechanism of action: Formation of a hemicetal adduct between the aldehyde group of the inhibitor and the active serine of the proteinase.
Inhibition spectrum: Inhibits papain, trypsin and plasmin to a lesser extent. More specific for papain and trypsin than leupeptin. The inhibitory potency of antipain is 100-fold higher than that of elastatinal.
Pale yellow powder; material is isolated from cultured media of microorganisms. As with many of these compounds, they are too inherently unstable to allow for high standards of purity. The homogeneity of these compounds cannot be guaranteed except for their specific inhibitory activities.
Properties: Soluble in H2O, methanol and DMSO (Stock solution: 10 mM). Stable at -20° C.
Mechanism of action: Formation of a hemicetal adduct between the aldehyde group of the inhibitor and the active serine of the proteinase.
Inhibition spectrum: Inhibits papain, trypsin and plasmin to a lesser extent. More specific for papain and trypsin than leupeptin. The inhibitory potency of antipain is 100-fold higher than that of elastatinal.
Pale yellow powder; material is isolated from cultured media of microorganisms. As with many of these compounds, they are too inherently unstable to allow for high standards of purity. The homogeneity of these compounds cannot be guaranteed except for their specific inhibitory activities.
Przechowywanie i stabilność
Maintain dry at -20ºC for up to 18 months. Store reconstituted product in aliquots at -20ºC for up to 9 months. Do not thaw and refreeze.
Informacje prawne
CHEMICON is a registered trademark of Merck KGaA, Darmstadt, Germany
Oświadczenie o zrzeczeniu się odpowiedzialności
Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.
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Kod klasy składowania
11 - Combustible Solids
Klasa zagrożenia wodnego (WGK)
WGK 3
Temperatura zapłonu (°F)
Not applicable
Temperatura zapłonu (°C)
Not applicable
Certyfikaty analizy (CoA)
Poszukaj Certyfikaty analizy (CoA), wpisując numer partii/serii produktów. Numery serii i partii można znaleźć na etykiecie produktu po słowach „seria” lub „partia”.
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Dokumenty związane z niedawno zakupionymi produktami zostały zamieszczone w Bibliotece dokumentów.
Structure of antipain, a new Sakaguchi-positive product of streptomyces.
S Umezawa et al.
The Journal of antibiotics, 25(4), 267-270 (1972-04-01)
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