AP180P
Donkey Anti-Goat IgG Antibody, HRP conjugate, Species Adsorbed
Chemicon®, from donkey
Synonim(y):
Anty-Rabbit IgG HRP, HRP IgG przeciw królikowi osła
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About This Item
Kod UNSPSC:
12352203
eCl@ss:
32160702
NACRES:
NA.46
Polecane produkty
pochodzenie biologiczne
donkey
Poziom jakości
białko sprzężone
peroxidase conjugate
forma przeciwciała
purified immunoglobulin
rodzaj przeciwciała
secondary antibodies
klon
polyclonal
reaktywność gatunkowa
goat
producent / nazwa handlowa
Chemicon®
metody
ELISA: suitable
immunohistochemistry: suitable
western blot: suitable
izotyp
IgG
Warunki transportu
wet ice
docelowa modyfikacja potranslacyjna
unmodified
Opis ogólny
Immunoglobulin G (IgG), is one of the most abundant proteins in human serum with normal levels between 8-17 mg/mL in adult blood. IgG is important for our defense against microorganisms and the molecules are produced by B lymphocytes as a part of our adaptive immune response. The IgG molecule has two separate functions; to bind to the pathogen that elicited the response and to recruit other cells and molecules to destroy the antigen. The variability of the IgG pool is generated by somatic recombination and the number of specificities in an individual at a given time point is estimated to be 1011 variants.
Zastosowanie
Donkey anti-Goat IgG Antibody, HRP conjugate, Species Adsorbed is an antibody against Donkey Goat IgG for use in ELISA, IH & WB.
Immunohistochemistry:
1:500-1:5,000 dilution can be used.
Optimal working dilutions must be determined by end user.
1:500-1:5,000 dilution can be used.
Optimal working dilutions must be determined by end user.
Jakość
Routinely tested on Enzyme-linked Immunosorbent Assay.
ELISA and Western Blots:
1:5,000-1:100,000 dilution can be used.
ELISA and Western Blots:
1:5,000-1:100,000 dilution can be used.
Postać fizyczna
Purified donkey secondary antibody IgG in lyophilized buffer 0.01 M Sodium Phosphate, 0.25 M NaCl, pH 7.6 with 15 mg/mL BSA.
Reconstitution:
Reconstitute with 500 μL of sterile distilled water.
Reconstitution:
Reconstitute with 500 μL of sterile distilled water.
Przechowywanie i stabilność
Maintain lyophilized product at 2-8°C for up to 12 months.
After reconstitution the product is stable for several weeks at 2-8°C as an undiluted liquid. For extended storage after reconstitution, add an equal volume of glycerol to make a final concentration of 50% glycerol followed by storage at -20°C in undiluted aliquots for up to 12 months. Please note the concentration of protein (and buffer salts) will decrease to one-half of the original after the addition of glycerol. Avoid repeated freeze/thaw cycles.
WARNING: Use of sodium azide as a preservative will substantially inhibit the enzyme activity of HRP.
After reconstitution the product is stable for several weeks at 2-8°C as an undiluted liquid. For extended storage after reconstitution, add an equal volume of glycerol to make a final concentration of 50% glycerol followed by storage at -20°C in undiluted aliquots for up to 12 months. Please note the concentration of protein (and buffer salts) will decrease to one-half of the original after the addition of glycerol. Avoid repeated freeze/thaw cycles.
WARNING: Use of sodium azide as a preservative will substantially inhibit the enzyme activity of HRP.
Informacje prawne
CHEMICON is a registered trademark of Merck KGaA, Darmstadt, Germany
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Kod klasy składowania
11 - Combustible Solids
Klasa zagrożenia wodnego (WGK)
WGK 3
Certyfikaty analizy (CoA)
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